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Erschienen in: Journal of Natural Medicines 2/2012

01.04.2012 | Original Paper

A rapid one-step immunochromatographic assay for the detection of asiaticoside

verfasst von: Boonchoo Sritularak, Thaweesak Juengwatanatrakul, Waraporn Putalun, Hiroyuki Tanaka, Satoshi Morimoto

Erschienen in: Journal of Natural Medicines | Ausgabe 2/2012

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Abstract

Asiaticoside has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 μg/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside in plant extracts.
Literatur
1.
Zurück zum Zitat Farnsworth NR, Bunyapraphatsara N (1992) Thai medicinal plants: recommended for primary health care system. Mahidol University, Thailand Farnsworth NR, Bunyapraphatsara N (1992) Thai medicinal plants: recommended for primary health care system. Mahidol University, Thailand
2.
Zurück zum Zitat James JT, Dubery IA (2009) Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) Urban. Molecules 14:3922–3941PubMedCrossRef James JT, Dubery IA (2009) Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) Urban. Molecules 14:3922–3941PubMedCrossRef
3.
Zurück zum Zitat Zhang XG, Han T, Zhang QY, Zhang H, Huang BK, Xu LL, Qin LP (2009) Chemical fingerprinting and hierarchical clustering analysis of Centella asiatica from different locations in China. Chromatographia 69:51–57CrossRef Zhang XG, Han T, Zhang QY, Zhang H, Huang BK, Xu LL, Qin LP (2009) Chemical fingerprinting and hierarchical clustering analysis of Centella asiatica from different locations in China. Chromatographia 69:51–57CrossRef
4.
Zurück zum Zitat Shukla A, Rasik AM, Jain GK, Shankar R, Kulshrestha DK, Dhawan BN (1999) In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J Ethnopharmacol 65:1–11PubMedCrossRef Shukla A, Rasik AM, Jain GK, Shankar R, Kulshrestha DK, Dhawan BN (1999) In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J Ethnopharmacol 65:1–11PubMedCrossRef
5.
Zurück zum Zitat Zhang L, Li HZ, Gong X, Luo FL, Wang B, Hu N, Wang CD, Zhang Z, Wan JY (2010) Protective effects of asiaticoside on acute liver injury induced by lipopolysaccharide/D-galactosamine in mice. Phytomedicine 17:811–819PubMedCrossRef Zhang L, Li HZ, Gong X, Luo FL, Wang B, Hu N, Wang CD, Zhang Z, Wan JY (2010) Protective effects of asiaticoside on acute liver injury induced by lipopolysaccharide/D-galactosamine in mice. Phytomedicine 17:811–819PubMedCrossRef
6.
Zurück zum Zitat Ryu JS, Park SG, Kwak TJ, Chang MY, Park ME, Choi KH, Sung KH, Shin HJ, Lee CK, Kang YS, Yoon MS, Rang MJ, Kim SJ (2005) Improving lip wrinkles: lipstick-related image analysis. Skin Res Technol 11:157–164PubMedCrossRef Ryu JS, Park SG, Kwak TJ, Chang MY, Park ME, Choi KH, Sung KH, Shin HJ, Lee CK, Kang YS, Yoon MS, Rang MJ, Kim SJ (2005) Improving lip wrinkles: lipstick-related image analysis. Skin Res Technol 11:157–164PubMedCrossRef
7.
Zurück zum Zitat Mook-Jung I, Shin JE, Yun SH, Huh K, Koh JY, Park HK, Jew SS, Jung MW (1999) Protective effects of asiaticoside derivatives against beta-amyloid neurotoxicity. J Neurosci Res 58:417–425PubMedCrossRef Mook-Jung I, Shin JE, Yun SH, Huh K, Koh JY, Park HK, Jew SS, Jung MW (1999) Protective effects of asiaticoside derivatives against beta-amyloid neurotoxicity. J Neurosci Res 58:417–425PubMedCrossRef
8.
Zurück zum Zitat Bonfill M, Mangas S, Cusidó RM, Osuna L, Piñol MT, Palazón J (2006) Identification of triterpenoid compounds of Centella asiatica by thin-layer chromatography and mass spectrometry. J Biomed Chromatogr 20:151–153CrossRef Bonfill M, Mangas S, Cusidó RM, Osuna L, Piñol MT, Palazón J (2006) Identification of triterpenoid compounds of Centella asiatica by thin-layer chromatography and mass spectrometry. J Biomed Chromatogr 20:151–153CrossRef
9.
Zurück zum Zitat Jain PK, Agrawal RK (2008) High performance liquid chromatographic analysis of asiaticoside in Centella asiatica (L.) Urban. Chiang Mai J Sci 35:521–552 Jain PK, Agrawal RK (2008) High performance liquid chromatographic analysis of asiaticoside in Centella asiatica (L.) Urban. Chiang Mai J Sci 35:521–552
10.
Zurück zum Zitat Verma RK, Bhartariya KG, Gupta MM, Kumar S (1999) Reverse-phase high performance liquid chromatography of asiaticoside in Centella asiatica. Phytochem Anal 10:191–193CrossRef Verma RK, Bhartariya KG, Gupta MM, Kumar S (1999) Reverse-phase high performance liquid chromatography of asiaticoside in Centella asiatica. Phytochem Anal 10:191–193CrossRef
11.
Zurück zum Zitat Juengwatanatrakul T, Sritularak B, Amornnopparattanakul P, Tassanawa P, Putalun W, Tanaka H, Morimoto S (2011) Preparation of a specific monoclonal antibody to asiaticoside for the development of an enzyme-linked immunosorbent assay. Analyst 136:1013–1017PubMedCrossRef Juengwatanatrakul T, Sritularak B, Amornnopparattanakul P, Tassanawa P, Putalun W, Tanaka H, Morimoto S (2011) Preparation of a specific monoclonal antibody to asiaticoside for the development of an enzyme-linked immunosorbent assay. Analyst 136:1013–1017PubMedCrossRef
12.
Zurück zum Zitat Shyu RH, Shyu HF, Liu HW, Tang SS (2002) Colloidal gold-based immunochromatographic assay for detection of ricin. Toxicon 40:255–258PubMedCrossRef Shyu RH, Shyu HF, Liu HW, Tang SS (2002) Colloidal gold-based immunochromatographic assay for detection of ricin. Toxicon 40:255–258PubMedCrossRef
13.
Zurück zum Zitat Putalun W, Tanaka H, Shoyama Y (2005) Quick detection of glycyrrhizin by an immunochromatographic assay. Phytochem Anal 16:370–374PubMedCrossRef Putalun W, Tanaka H, Shoyama Y (2005) Quick detection of glycyrrhizin by an immunochromatographic assay. Phytochem Anal 16:370–374PubMedCrossRef
14.
Zurück zum Zitat Imsungnoen N, Phrompittayarat W, Ingkaninan K, Tanaka H, Putalun W (2009) Immunochromatographic assay for the detection of pseudojujubogenin glycosides. Phytochem Anal 20(1):64–67PubMedCrossRef Imsungnoen N, Phrompittayarat W, Ingkaninan K, Tanaka H, Putalun W (2009) Immunochromatographic assay for the detection of pseudojujubogenin glycosides. Phytochem Anal 20(1):64–67PubMedCrossRef
15.
Zurück zum Zitat Randriamampionona D, Diallo B, Rakotonirian F, Rabemanantsoa C, Cheuk K, Corbisier AM, Mahillon J, Ratsimamanga S, EI Jaziri M (2007) Comparative analysis of active constituents in Centella asiatica samples from Madagascar: application for ex situ conservation and clonal propagation. Fitoterapia 78:482–489PubMedCrossRef Randriamampionona D, Diallo B, Rakotonirian F, Rabemanantsoa C, Cheuk K, Corbisier AM, Mahillon J, Ratsimamanga S, EI Jaziri M (2007) Comparative analysis of active constituents in Centella asiatica samples from Madagascar: application for ex situ conservation and clonal propagation. Fitoterapia 78:482–489PubMedCrossRef
Metadaten
Titel
A rapid one-step immunochromatographic assay for the detection of asiaticoside
verfasst von
Boonchoo Sritularak
Thaweesak Juengwatanatrakul
Waraporn Putalun
Hiroyuki Tanaka
Satoshi Morimoto
Publikationsdatum
01.04.2012
Verlag
Springer Japan
Erschienen in
Journal of Natural Medicines / Ausgabe 2/2012
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-011-0582-2

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