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Angiogenesis

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17.01.2019 | Brief Communication

A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis

verfasst von: Yunwei Shi, Xuchu Duan, Guangmin Xu, Xiaoning Wang, Guanyun Wei, Shikui Dong, Gangcai Xie, Dong Liu

Erschienen in: Angiogenesis | Ausgabe 2/2019

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Abstract

MicroRNAs (miRNAs) are single-stranded small non-coding RNAs, generally 18–25 nucleotides in length, that act as repressors of gene expression. miRNAs are encoded by independent genes or processed from a variety of different RNA species. So far, there is no evidence showing that the ribosomal DNA-hosted microRNA is implicated in vertebrate development. Currently, we found a highly expressed small RNA hosted in ribosomal DNA was predicted as a novel miRNA, named miR-ntu1, in zebrafish endothelial cells by deep sequencing analysis. The miRNA was validated by custom-designed Taqman PCR, Northern Blot, and in silico analysis. Furthermore, we demonstrated that miR-ntu1 played a crucial role in zebrafish angiogenesis via modulation of Notch signaling. Our findings provide a notable case that a miRNA hosted in ribosomal DNA is involved in vertebrate development.

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Titel: A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis
verfasst von: Yunwei Shi
Xuchu Duan
Guangmin Xu
Xiaoning Wang
Guanyun Wei
Shikui Dong
Gangcai Xie
Dong Liu
Publikationsdatum: 17.01.2019
Verlag: Springer Netherlands
Erschienen in: Angiogenesis / Ausgabe 2/2019
Print ISSN: 0969-6970
Elektronische ISSN: 1573-7209
DOI: https://doi.org/10.1007/s10456-019-09663-3

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