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Archives of Virology

Erschienen in:

01.04.2011 | Brief Report

Absence of infection in pigs inoculated with high-titre recombinant PERV-A/C

verfasst von: D. Kaulitz, D. Mihica, R. Plesker, A. Geissler, R. R. Tönjes, J. Denner

Erschienen in: Archives of Virology | Ausgabe 4/2011

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Abstract

Porcine endogenous retroviruses (PERVs) represent a risk for xenotransplantation using pig cells or organs since they are integrated in the genome of all pigs and infect human cells in vitro. Recombinants between PERV-A and PERV-C have been described in pigs in vivo and found de novo integrated in the genome of somatic cells, but not in the germ line. To study whether PERV-A/C can infect and have a pathogenic effect in normal pigs, German landrace pigs were inoculated with high-titre PERV-A/C. No provirus integration was found in blood cells or in various tissues, and no antibody production was observed, indicating the absence of infection.

Wilson CA (2008) Porcine endogenous retroviruses and xenotransplantation. Cell Mol Life Sci 65:3399–3412PubMedCrossRef

Wilson C, Wong S, VanBrocklin M, Federspiel M (2000) Extended analysis of the in vitro tropism of porcine endogenous retrovirus. J Virol 74:49–56PubMedCrossRef

Wood JC, Quinn G, Suling KM, Oldmixon BA, Van Tine BA, Cina R, Arn S, Huang CA, Scobie L, Onions DE, Sachs DH, Schuurman HJ, Fishman JA, Patience C (2000) Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine. J Virol 78:2494–2501CrossRef

Bartosch B, Stefanidis D, Myers R, Weiss R, Patience C, Takeuchi Y (2004) Evidence and consequence of porcine endogenous retrovirus recombination. J Virol 78:13880–13890PubMedCrossRef

Martin SI, Wilkinson R, Fishman JA (2006) Genomic presence of recombinant porcine endogenous retrovirus in transmitting miniature swine. J Virol 3:91–96CrossRef

Denner J (2008) Recombinant porcine endogenous retroviruses (PERV-A/C): a new risk for xenotransplantation? Arch Virol 153:1421–1426PubMedCrossRef

Harrison I, Takeuchi Y, Bartosch B, Stoye JP (2004) Determinants of high titer in recombinant porcine endogenous retroviruses. J Virol 78:13871–13879PubMedCrossRef

Denner J, Specke V, Thiesen U, Karlas A, Kurth R (2003) Genetic alterations of the long terminal repeat of an ecotropic porcine endogenous retrovirus (PERV) during passage in human cells. Virology 314:125–133PubMedCrossRef

Scheef G, Fischer N, Krach U, Tönjes RR (2001) The number of a U3 repeat box acting as an enhancer in long terminal repeats of polytropic replication-competent porcine endogenous retroviruses dynamically fluctuates during serial virus passages in human cells. J Virol 75(15):6933–6940PubMedCrossRef

10.

Scheef G, Fischer N, Flory E, Schmitt I, Tönjes RR (2002) Transcriptional regulation of porcine endogenous retroviruses released from porcine and infected human cells by heterotrimeric protein complex NF-Y and impact of immunosuppressive drugs. J Virol 76(24):12553–12563PubMedCrossRef

11.

Suzuka I, Sekiguchi K, Kodama M (1985) Some characteristics of a porcine retrovirus from a cell line derived from swine malignant lymphomas. FEBS Lett 183:124–128PubMedCrossRef

12.

Dieckhoff B, Puhlmann J, Büscher K, Hafner-Marx A, Herbach N, Bannert N, Büttner M, Wanke R, Kurth R, Denner J (2007) Expression of porcine endogenous retroviruses (PERVs) in melanomas of Munich miniature swine (MMS). Troll Vet Microbiol 123:53–68CrossRef

13.

Breese SS Jr (1970) Virus-like particles occurring in cultures of stable pig kidney cell lines. Arch Gesamte Virusforsch 30:401–404PubMedCrossRef

14.

Armstrong JA, Porterfield JS, Madrid ATD (1971) C-type virus particles in pig kidney cell lines. J Gen Virol 10:195–198PubMedCrossRef

15.

Karlas A, Irgang M, Votteler J, Specke V, Özel M, Kurth R, Denner J (2010) Characterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/C. Ann Transplant 15(2):45–54PubMed

16.

Le Tissier P, Stoye JP, Takeuchi C, Patience YC, Weiss RA (1997) Two sets of human-tropic pig retrovirus. Nature 389:681–682PubMedCrossRef

17.

Tacke S, Bodusch K, Berg A, Denner J (2001) Sensitive and specific immunological detection methods for porcine endogenous retroviruses applicable to experimental and clinical xenotransplantation. Xenotransplantation 8:125–135PubMedCrossRef

18.

Irgang M, Sauer IM, Karlas A, Zeilinger K, Gerlach JC, Kurth R, Neuhaus P, Denner J (2003) Porcine endogenous retroviruses (PERVs): no infection in patients treated with a bioreactor based on porcine liver cells. J Clin Virol 28:141–154PubMedCrossRef

19.

Garkavenko O, Dieckhoff B, Wynyard S, Denner J, Elliott RB, Tan PL, Croxson MC (2008) Absence of transmission of potentially xenotic viruses in a prospective pig to primate islet xenotransplantation study. J Med Virol 80:2046–2052PubMedCrossRef

20.

Specke V, Plesker R, Coulibaly C, Boller K, Denner J (2002) Productive infection of a mink cell line with porcine endogenous retroviruses (PERVs) and lack of transmission to minks in vivo. Arch Virol 147:305–319PubMedCrossRef

21.

Ritzhaupt A, van der Laan LJW, Salomon DR, Wilson CA (2002) Porcine endogenous retrovirus infects but does not replicate in nonhuman primate cells and cell lines. J Virol 76:11312–11320PubMedCrossRef

22.

Blusch JH, Patience C, Takeuchi Y, Templin C, Roos C, Von Der Helm K, Steinhoff G, Martin U (2000) Infection of nonhuman primate cells by pig endogenous retrovirus. J Virol 74:7687–7690PubMedCrossRef

23.

Specke V, Plesker R, Wood J, Coulibaly C, Suling K, Patience C, Kurth R, Schuurman HJ, Denner J (2009) No in vivo infection of triple immunosuppressed non-human primates after inoculation with high titers of porcine endogenous retroviruses (PERVs). Xenotransplantation 16:34–44PubMedCrossRef

24.

Lachmann PJ, Davies A (1997) Complement and immunity to viruses. Immunol Rev 159:69PubMedCrossRef

25.

Ochsenbein AF, Zinkernagel RM (2000) Natural antibodies and the complement link innate and acquired immunity. Immunol Today 21:624PubMedCrossRef

26.

Towers GJ (2007) The control of viral infection by tripartite motif proteins and cyclophilin A. Retrovirology 4:40PubMedCrossRef

27.

Goilar-Gaur R, Strebel K (2008) HIV-1 Vif, APOBEC, and intrinsic immunity. Retrovirology 5:51CrossRef

28.

Dörrschuck E, Münk C, Tönjes RR (2008) APOBEC3 proteins and porcine endogenous retroviruses. Transplant Proc 40(4):959–961PubMedCrossRef

29.

Torres AN, O’Halloran KP, Larson LJ, Schultz RD, Hoover EA (2010) Feline leukemia virus immunity induced by whole inactivated virus vaccination. Vet Immunol Immunopathol 134(1–2):122–131PubMedCrossRef

30.

Major A, Cattori V, Boenzli E, Riond B, Ossent P, Meli ML, Hofmann-Lehmann R, Lutz H (2010) Exposure of cats to low doses of FeLV: seroconversion as the sole parameter of infection. Vet Res 41(2):17PubMedCrossRef

31.

Evans LH, Lavignon M, Peterson K, Hasenkrug K, Robertson S, Malik F, Virtaneva K (2006) In vivo interactions of ecotropic and polytropic murine leukemia viruses in mixed retrovirus infections. J Virol. 80(10):4748–4757PubMedCrossRef

32.

Büscher K, Trefzer U, Hofman M, Sterry W, Kurth R, Denner J (2005) Expression of human endogenous retrovirus K in melanomas and melanoma cell lines. Cancer Res 65(10):4172–4180PubMedCrossRef

33.

Denner J, Schuurman H, Patience C (2009) The International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes—chapter 5: strategies to prevent transmission of porcine endogenous retroviruses. Xenotransplantation 16:239–248PubMedCrossRef

Titel: Absence of infection in pigs inoculated with high-titre recombinant PERV-A/C
verfasst von: D. Kaulitz
D. Mihica
R. Plesker
A. Geissler
R. R. Tönjes
J. Denner
Publikationsdatum: 01.04.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-010-0896-5

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