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Journal of Cancer Research and Clinical Oncology

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01.04.2013 | Original Paper

ALY as a potential contributor to metastasis in human oral squamous cell carcinoma

verfasst von: Yasuhiro Saito, Atsushi Kasamatsu, Ayumi Yamamoto, Toshihiro Shimizu, Hidetaka Yokoe, Yosuke Sakamoto, Katsunori Ogawara, Masashi Shiiba, Hideki Tanzawa, Katsuhiro Uzawa

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 4/2013

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Abstract

Purpose

ALY, an essential mRNA export factor, is dysregulated in a wide variety of human malignancies. However, little is known about the relevance of ALY to oral squamous cell carcinoma (OSCC). The purpose of this study was to investigate ALY expression and its functional mechanisms in OSCCs.

Methods

ALY mRNA and protein expression in seven OSCC-derived cell lines (Sa3, HO-1-u-1, KON, Ca9-22, HSC-2, HSC-3, and HSC-4) and primary OSCCs were analyzed by quantitative reverse transcriptase-polymerase chain reaction, immunoblotting, and immunohistochemistry. We evaluated cellular invasiveness, migration, and the expression levels of metastasis modulators, ribosomal RNA processing 1 homolog B (RRP1B) and CD82, in ALY knockdown cells.

Results

ALY was frequently up-regulated in OSCC-derived cell lines and primary OSCCs compared with normal counterparts at both the mRNA and protein expression levels. ALY-positive expression was correlated significantly (P < 0.05) with a higher risk of regional lymph node metastasis. Furthermore, ALY knockdown cells caused a significant (P < 0.05) decrease in cellular invasiveness and migration with up-regulation of RRP1B and CD82 compared with the control cells.

Conclusion

Our results showed that ALY is linked to regional lymph node metastasis by regulating cellular invasiveness and migration. Therefore, ALY might be a potential biomarker for early detection of lymph node metastasis in OSCCs.

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Titel: ALY as a potential contributor to metastasis in human oral squamous cell carcinoma
verfasst von: Yasuhiro Saito
Atsushi Kasamatsu
Ayumi Yamamoto
Toshihiro Shimizu
Hidetaka Yokoe
Yosuke Sakamoto
Katsunori Ogawara
Masashi Shiiba
Hideki Tanzawa
Katsuhiro Uzawa
Publikationsdatum: 01.04.2013
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 4/2013
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-012-1361-5

Springer Medizin

ALY as a potential contributor to metastasis in human oral squamous cell carcinoma