Amplification and overexpression of PSCA at 8q24 in invasive micropapillary carcinoma of breast
- 28.07.2017
- Preclinical study
Erschienen in:
- Verfasst von
- Fanfan Meng
- Bingbing Liu
- Gan Xie
- Yawen Song
- Xia Zheng
- Xiaolong Qian
- Shuai Li
- Hongqin Jia
- Xinmin Zhang
- Lanjing Zhang
- Yi-ling Yang
- Li Fu
- Erschienen in
- Breast Cancer Research and Treatment | Ausgabe 2/2017
Abstract
Purpose
Invasive micropapillary carcinoma (IMPC) of the breast has distinct histological features and molecular genetic profiles. Gains/amplifications of 8q24 are found associated with IMPC. Although the prostate stem cell antigen (PSCA) gene is located at chromosome 8q24, and found over-expressed in prior studies, its prognostic values and biological significance in IMPC have not been well studied.
Methods
Fluorescence in situ hybridization (FISH) was used to assess the frequencies of PSCA copy number gains in IMPC, invasive ductal carcinoma of no special type (IDC-NST), and invasive lobular carcinoma (ILC) samples. The protein expression levels of PSCA were examined in 56 IMPC, 72 IDC-NST, and 56 ILC samples using immunohistochemical analysis.
Results
PSCA gene amplification was detected in 45.2% (14/31) of the IMPC, 28.1% (9/32) of the IDC-NST, and none (0/25) of the ILC. PSCA protein expression was observed in 58.9% (33/56), 40.3% (29/72), and 3.6% (2/56) of IMPC, IDC-NST, and ILC samples, respectively. The concordant rate of the immunohistochemistry and FISH data was 85.2%. PSCA gene amplification highly correlated with its protein overexpression (rs = 0.687, P < 0.001), suggesting that gene amplification is an important mechanism involved in PSCA overexpression. Our univariate analysis showed that the patients with PSCA-positive IMPC had a decreased disease-free survival (DFS) compared to PSCA-negative IMPC patients (P = 0.003). Our multivariate analysis confirmed the worse DFS in PSCA-positive IMPC patients (P = 0.022).
Conclusions
Our results indicate that PSCA may be an attractive target in the 8q24 amplicon and that it may serve as a molecular marker of metastasis and recurrence in IMPC. The differential expression of PSCA may be associated with cell adhesion. Detection of PSCA protein and gene amplification may help manage and predict the prognosis of IMPC patients.
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- Titel
- Amplification and overexpression of PSCA at 8q24 in invasive micropapillary carcinoma of breast
- Verfasst von
-
Fanfan Meng
Bingbing Liu
Gan Xie
Yawen Song
Xia Zheng
Xiaolong Qian
Shuai Li
Hongqin Jia
Xinmin Zhang
Lanjing Zhang
Yi-ling Yang
Li Fu
- Publikationsdatum
- 28.07.2017
- Verlag
- Springer US
- Erschienen in
-
Breast Cancer Research and Treatment / Ausgabe 2/2017
Print ISSN: 0167-6806
Elektronische ISSN: 1573-7217 - DOI
- https://doi.org/10.1007/s10549-017-4407-1
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