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Urolithiasis

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01.12.2005 | Original Paper

Analysis of urinary calculi obtained from a patient with idiopathic hypouricemia using micro area x-ray diffractometry and LC-MS

verfasst von: Kiyoko Kaneko, Tomoyo Yamanobe, Maki Onoda, Ken-ichi Mawatari, Kazuya Nakagomi, Shin Fujimori

Erschienen in: Urolithiasis | Ausgabe 6/2005

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Abstract

Urolithiasis is a common complication in patients with hypouricemia. Using a microarea x-ray diffractometer and nanoflow liquid chromatography-mass spectrometry (LC-MS) following SDS-polyacrylamide gel electrophoresis (PAGE), recurrent urinary calculi complicating a hypouricemic patient were analyzed. Analysis with the microarea x-ray diffractometer showed that one of the calculi was composed of calcium oxalate monohydrate and hydroxyapatite. The other was found to be formed from calcium oxalate dihydrate. After determination with LC-MS, both were found to contain uromodulin, albumin, osteopontin, protein Z, and defensins. Lysozyme and calgranulin A were also identified in these calculi. Defensins, which were antimicrobial peptides, and lysozyme, a mucopeptide glycohydrolase, were identified as new organic components of urinary stones. The role of these proteins in the process of urolithiasis is of particular interest.

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Titel: Analysis of urinary calculi obtained from a patient with idiopathic hypouricemia using micro area x-ray diffractometry and LC-MS
verfasst von: Kiyoko Kaneko
Tomoyo Yamanobe
Maki Onoda
Ken-ichi Mawatari
Kazuya Nakagomi
Shin Fujimori
Publikationsdatum: 01.12.2005
Verlag: Springer-Verlag
Erschienen in: Urolithiasis / Ausgabe 6/2005
Print ISSN: 2194-7228
Elektronische ISSN: 2194-7236
DOI: https://doi.org/10.1007/s00240-005-0480-8

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