Background
Methods
Chemicals and reagents
Plant material
Preparation of extraction and fractionation
Animals
Evaluation of anti-hyperuricemic effect
Drug administration
Animal hyperuricemia model
Determination of blood uric acid levels
Enzyme preparation from rat liver
Assay for XO inhibition in rat liver
Evaluation of anti-inflammatory effect
Drug administration
Carrageenan-induced rat paw edema
Carrageenan-induced mechanical hyperalgesia
Carrageenan-induced thermal hyperalgesia
Urate-induced synovitis
Statistical data analysis
Determination of total phenolic content
Characterization of phenolic compounds
Results
Effect of CEE on serum uric acid levels in rats
Group treatment | Dose (mg/kg) | Number | Serum uric acid levels (μmol/L) | Inhibition (%) |
---|---|---|---|---|
Normal | 116.17 ± 8.71 | |||
Control | 8 | 218.66 ± 16.82##
| ||
CEE | 300 | 8 | 174.49 ± 12.31 | 20.2 |
600 | 8 | 159.16 ± 16.36* | 27.2 | |
1200 | 8 | 158.18 ± 14.07* | 27.7 | |
Allopurinol | 10 | 8 | 101.69 ± 15.98** | 53.5 |
Effect of CEE and BuOH fraction on serum uric acid levels in rats
Group treatment | Number | Dose (mg/kg) | Serum uric acid levels (μmol/L) | Inhibition (%) |
---|---|---|---|---|
Normal | 8 | 114.54 ± 11.23 | ||
Control | 8 | 222.38 ± 24.58##
| ||
CEE | 8 | 600 | 163.58 ± 12.94* | 26.4 |
BuOH fr. | 8 | 120 | 152.47 ± 11.68** | 31.4 |
Allopurinol | 8 | 10 | 115.28 ± 11.13** | 48.2 |
Effect of BuOH fraction on XO activity in rats
Effect of BuOH fraction on paw edema in rats
Effect of BuOH fraction on mechanical hyperalgesia in rats
Effect of BuOH fraction on thermal hyperalgesia in rats
Effect of BuOH fraction on synovitis
Group | Number | Dose (mg/kg p.o.) | Score |
---|---|---|---|
Control | 8 | - | 3.5 (2–4) |
BuOH fr. | 8 | 120 | 2 (1–3)* |
Indomethacin | 8 | 10 | 1 (1–1)** |