Skip to main content
main-content

01.12.2016 | Short Communication | Ausgabe 1/2016 Open Access

Autoimmunity Highlights 1/2016

Anti-rods/rings autoantibody seropositivity does not affect response to telaprevir treatment for chronic hepatitis C infection

Zeitschrift:
Autoimmunity Highlights > Ausgabe 1/2016
Autoren:
S. John Calise, Nicola Bizzaro, Thuy Nguyen, Danila Bassetti, Brunetta Porcelli, Paolo Almi, Giuseppina Barberio, Giampaola Pesce, Minoru Satoh, Edward K. L. Chan
Abbreviations
ANA
Antinuclear antibody
anti-RR
Anti-rods/rings autoantibody
DAAs
Direct-acting antivirals
HCV
Hepatitis C
IFN
Interferon-α
IFN/RBV
Interferon-α and ribavirin therapy
IMPDH
Inosine 5′-monophosphate dehydrogenase
RBV
Ribavirin
RRs
Rods and rings
SVR
Sustained virological response
TPV
Telaprevir

Introduction

Chronic hepatitis C (HCV) infection is associated with the production of autoantibodies, including organ-specific autoantibodies directed against targets in the thyroid, adrenal cortex, pancreatic islet cells, and gastric parietal cells, and non-organ-specific autoantibodies such as antinuclear, anti-smooth muscle, anti-mitochondrial, anti-liver/kidney microsomal, and anti-neutrophil cytoplasmic antibodies [ 18]. Recent studies have also demonstrated a link between HCV and the production of autoantibodies targeting intracellular filamentous structures termed ‘rods and rings’ (RRs) [ 916]. In most studies, anti-rods/rings (anti-RR) seropositivity appears to be almost exclusive to HCV patients treated with interferon-α and ribavirin combination therapy (IFN/RBV) and is rarely seen in treatment-naïve HCV patients or other disease groups. However, anti-RR has been observed in one hepatitis B patient [ 11], one systemic lupus erythematosus patient [ 17], and healthy individuals with no previous IFN/RBV treatment [ 17]. In cultured cells, RRs are composed of inosine 5′-monophosphate dehydrogenase (IMPDH), and/or cytidine 5′-triphosphate synthase under certain conditions [ 9, 18, 19]. RRs tend to assemble when de novo purine biosynthesis is inhibited and guanine nucleotide levels become depleted [ 2025]. Many patients with anti-RR react with IMPDH, which is inhibited by direct binding to RBV and appears to be the major autoantigen in RRs [ 9, 26, 27]. Although no mechanistic evidence suggesting that anti-RR autoantibody contributes to resistance to IFN/RBV therapy has yet been reported, previous studies showed that anti-RR antibodies were more prevalent in patients who did not respond to therapy or relapsed, when compared to sustained responders [ 10, 15]. Additionally, non-responsive or relapsing patients had higher anti-RR titers, suggesting that anti-RR positivity may be indicative of poor treatment outcomes [ 28]. In recent years, direct-acting antivirals (DAAs), such as telaprevir (TPV), have been developed for chronic HCV infection in an effort to reduce therapy duration and increase drug tolerability, while also improving patient outcomes. Currently, TPV is included with IFN/RBV as a triple therapy. Here, we examine the relationship between anti-RR and treatment outcomes in a cohort of Italian patients treated with IFN/RBV and telaprevir.

Methods

Patient and treatment information

From 2013 to 2014, 52 HCV-infected patients were treated with IFN/RBV and TPV at five Italian clinics located at the (1) Ospedale San Antonio, Tolmezzo, (2) Ospedale Santa Chiara, Trento, (3) Università degli Studi di Siena, (4) Ospedale Regionale, Treviso, and (5) Università degli Studi di Genova. Dosages depended on patient weight (75 kg discriminating weight) and were typically administered as follows: 80–180 µg weekly pegylated interferon-α, 600–1400 mg daily ribavirin, and 2250 mg daily TPV. Patients were classified as: not responsive to therapy (HCV RNA still detectable at week 24 of therapy), relapsed (HCV RNA detectable after the end of treatment in patients with previous virological response), or responsive to therapy (HCV RNA not detectable in the 24 weeks after the completion of therapy). The study conforms to the Institutional Review Board requirements in all institutions.
Informed consent Informed consent was obtained from all individual participants included in the study. All patients provided written informed consent to receive IFN/RBV/TPV and permission for use of their medical records for this study.

Antinuclear antibody indirect immunofluorescence assay (ANA-IIF)

Anti-rods/rings in patient sera were detected by indirect immunofluorescence, using NOVA Lite HEp-2 ANA substrate (INOVA Diagnostics, San Diego, CA: 508100) as previously described [ 10]. Staining patterns of test sera were compared to staining of human prototype anti-RR serum It2006 used in previous studies [ 9, 10]. It2006 and all anti-RR-positive sera described in this study correctly recognize the rods and rings ANA pattern, designated as pattern AC-23 by the International Consensus on ANA Patterns (ICAP) [ 29]. All sera were tested at a dilution of 1:80 in PBS as previously described [ 30]. For anti-RR-positive patients who also had serial samples available, anti-RR end point titers were determined using twofold serial dilution of sera in PBS, with a starting dilution of 1:80 and ending dilution of 1:1280. Anti-RR positivity and titers were independently validated by two trained individuals (S.J.C. and T.N.). End point titer was defined by more than 50% of cells containing detectable RR staining. Donkey anti-human IgG conjugated to DyLight 488 (Jackson ImmunoResearch, West Grove, PA) diluted 1:100 in PBS was used to detect autoantibody staining. Fluorescent images were captured with a Zeiss Axiovert 200 M microscope fitted with a Zeiss AxioCam MRm camera using a 40× (0.75 NA) objective (Carl Zeiss Microscopy, Jena, Germany).

Statistical analysis

Biological and clinical variables analyzed for statistical significance include: gender, age, HCV genotype, previous treatment with IFN/RBV (prior to beginning of TPV regimen), and treatment outcome (see Table  1). Mann–Whitney U test was used to compare different groups containing continuous data, and Fisher’s exact test or the Fisher–Freeman–Halton exact test was used for categorical data. Differences were considered statistically significant if p < 0.05. Mann–Whitney U test and Fisher’s exact test were performed using GraphPad Prism 5.0 (GraphPad Software, La Jolla, CA). Fisher–Freeman–Halton test was performed using StatXact 10 (Cytel, Cambridge, MA).
Table 1
Summary of anti-RR autoantibody reactivity in HCV patients treated with interferon-α/ribavirin and telaprevir
Parameters
Total patients ( n = 52)
Anti-RR-positive patients ( n = 10)
Anti-RR-negative patients ( n = 42)
p value
Male
36 (69%)
7 (70%)
29 (69%)
NS
Female
16 (31%)
3 (30%)
13 (31%)
NS
Age (years) ± SD
54 ± 9
53 ± 14
54 ± 8
NS
Genotype 1a #
12 (23%)
3 (30%)
9 (21%)
NS
Genotype 1b #
39 (75%)
7 (70%)
32 (76%)
NS
Previous IFN/RBV
32 (62%)
10 (100%)
22 (52%)
<0.01
Treatment outcome
 SVR, no side effects
35 (67%)
6 (60%)
29 (69%)
NS
 SVR, but side effects
5 (10%)
1 (10%)
4 (10%)
NS
 SVR, combined
40 (77%)
7 (70%)
33 (79%)
NS
 Relapse
5 (10%)
3 (30%)
2 (5%)
<0.05
 No response
7 (13%)
0 (0%)
7 (17%)
NS
Fisher–Freeman–Halton exact test
   
Anti-RR positive vs. anti-RR negative, with SVR separated
 
NS
Anti-RR positive vs. anti-RR negative, with SVR combined
 
<0.05
Values presented as n (%) unless otherwise indicated
Anti-RR anti-rods/rings autoantibody, IFN/RBV interferon-α/ribavirin therapy, NS not statistically significant ( p > 0.05), SD standard deviation, SVR sustained virological response
#One patient without anti-RR was genotype 3b

Results and discussion

The goal of this study was to probe for an association between the presence of anti-RR autoantibody and treatment outcome in a cohort of 52 Italian HCV patients treated with the new DAA telaprevir. This is the first study to examine anti-RR in patients treated with any of the recently approved DAAs. It must be pointed out that, in general, the availability of patients for studies of anti-RR antibody is naturally limited by the low prevalence of this autoantibody response. Although it has been reported that 20–40% of HCV patients treated with IFN/RBV produce anti-RR autoantibodies, this response is very rarely observed in treatment-naïve HCV patients or other disease groups [ 10, 11, 1416, 31].
A total of 52 HCV-infected patients were treated with IFN/RBV and TPV. Thirty-six patients (69%) were male and 16 (31%) were female, with a mean age of 54 ± 9 years. Twelve patients (23%) had genotype 1a, 39 patients (75%) had genotype 1b, and one patient (2%) had genotype 3b. Thirty-two patients (62%) had been previously treated with IFN/RBV prior to being put on TPV. Patient demographics are included in Table  1. All 52 patients were assayed for the presence of anti-RR according to standard antinuclear antibody indirect immunofluorescence (ANA-IIF) protocols using HEp-2 cells as a substrate. ANA-IIF analysis revealed that 10 out of 52 patients (19%) were positive for anti-RR at a dilution of 1:80. Figure  1 displays the images of the ‘rods and rings’ staining pattern from all ten anti-RR-positive patients (patient codes C1TN, FV1S, TBN1S, VA1S, TG1TO, SL1G, VG1G, MS1G, RC1G, and CB1G). Anti-RR-negative patients CD2T and E1TN are also shown for comparison. Twenty-one out of 52 patients had serial collections of sera available (104 total sera for 52 patients), representing multiple visits to the clinic over time periods ranging from 2 weeks to 13 months. Anti-RR status did not change over time in any of these 21 patients (i.e., anti-RR-positive patients remained positive and negative patients remained negative) and anti-RR titers did not significantly change in positive patients. Accordingly, for purposes of statistical analysis, patients were simply considered either positive or negative, and serial collections were not taken into consideration.
Patients were divided into two main groups, anti-RR-positive ( n = 10) and anti-RR-negative ( n = 42), for subsequent statistical analysis. These groups were compared based on several demographic, clinical, and serological parameters to determine any differences between anti-RR-positive and anti-RR-negative patients (Table  1). There was no significant difference observed between the two groups when comparing gender, age, or HCV genotype. Both patient groups were also categorized into four different treatment outcome groups: (1) sustained virological response (SVR) with no side effects, (2) initial SVR but therapy was discontinued due to side effects, (3) relapse within 6 months of treatment, and (4) no response to treatment. SVR patients with or without side effects were all determined to be SVR at the same time point (after 1 month, according to international guidelines). When the Fisher–Freeman–Halton exact test was performed to compare anti-RR-positive vs. anti-RR-negative patient groups based on all four treatment outcome parameters, no significant difference was observed. However, additional Fisher–Freeman–Halton analysis with all SVR patients combined (regardless of side effects) resulted in a statistically significant p value <0.05 (Table  1, bottom row). Thus, our data indicate that anti-RR-positive and anti-RR-negative patients in this cohort appear to differ with regard to distribution of treatment outcomes. The most notable difference is that anti-RR-positive patients were more likely to relapse than anti-RR-negative patients ( p < 0.05). Despite the increase in relapse, 0 of the 10 anti-RR-positive patients were non-responsive to therapy, compared to 7 of the 42 (17%) anti-RR-negative patients ( p = 0.32). Additionally, there was no significant difference in SVR rates between both groups. SVR rates were compared with patients who experienced side effects and those who did not separated as different outcomes (“SVR, but side effects” and “SVR, no side effects”) or with all SVR patients combined (“SVR, combined”), but both analyses showed no significant difference between anti-RR-positive and anti-RR-negative patients.
We also discovered that anti-RR can be detected years after treatment with IFN/RBV. Two of the ten anti-RR-positive patients had serum collected both prior to and after IFN/RBV/TPV therapy was initiated. All samples from both patients were positive for anti-RR, initially suggesting that these patients may have been positive with no previous IFN/RBV treatment. However, after careful examination of medical records, it was determined that both patients had received IFN/RBV more than a decade prior to treatment with TPV. Patient TBN1S was diagnosed with liver cirrhosis in 2000 and treated with IFN/RBV from 2000 to 2002, but treatment was eventually discontinued due to side effects. In 2014, TBN1S began receiving IFN/RBV again with addition of TPV, but relapsed within 6 months. Patient VA1S previously received IFN/RBV for 6 months in 2003. Eleven years later, in 2014, the patient began receiving IFN/RBV again with addition of TPV, but therapy was discontinued due to side effects. Despite the lack of exposure to IFN/RBV for more than 10 years, both patients remained positive for anti-RR autoantibody, and VA1S even remained positive down to 1:1280 dilution. We speculate that long-lived plasma cells might be responsible for the long-term presence of anti-RR antibody in these patients. Previous studies have suggested that anti-RR titer increases throughout the duration of therapy, but declines upon cessation of treatment [ 11, 12, 15, 31]. To our knowledge, this is the first report of long-lived anti-RR autoantibody.
Previous studies established a strong association between anti-RR and IFN/RBV therapy [ 1012, 14, 15], such that we previously described anti-RR as a drug-induced autoantibody [ 13]. Additionally, prolonged exposure to IFN/RBV increases the likelihood of anti-RR autoantibody production [ 11, 12, 15]. Our data support these findings, considering that 10 out of 32 (31%) patients previously treated with IFN/RBV were anti-RR positive, compared to 0 out of 20 patients who had not previously received IFN/RBV. In terms of treatment outcome, previous reports have indicated a link between anti-RR and non-responsiveness or relapse in American and Italian HCV patient cohorts [ 10, 15, 28]. In the current study with a new cohort of Italian patients, we again found that anti-RR seropositivity was associated with increased frequency of relapse. Interestingly, the frequency of non-responsiveness appeared to be decreased in anti-RR-positive patients (0/10, 0%) compared to anti-RR-negative patients (7/42, 17%), despite the opposite trend in relapse. When patients with no previous IFN/RBV treatment are removed from analysis, the trend remains similar, with non-responsiveness occurring in 0/10 (0%) of anti-RR-positive patients compared to 6/16 (27%) of anti-RR-negative patients ( p = 0.14). Overall, 19% (10/52) of HCV patients in this study were positive for anti-RR, which is similar to the rates observed in previous studies [ 10, 11, 1416]. Importantly, the addition of TPV to the IFN/RBV regimen did not induce a new anti-RR response in any patients; anti-RR-negative patients previously treated with IFN/RBV did not become positive after TPV was included in the regimen. Likewise, anti-RR titers did not significantly change after addition of TPV in anti-RR-positive patients with serial samples available. In all, our data suggest that inclusion of TPV in the IFN/RBV regimen for the treatment of chronic HCV does not alter the production of anti-RR autoantibody. However, based on the statistically significant increase in relapse rate, it may be useful to carefully monitor anti-RR-positive patients during and for 6 months after IFN/RBV/TPV therapy. While our study is limited by the number of available patients, the data indicate that anti-RR seropositivity does not affect the response to TPV treatment for chronic HCV.

Acknowledgements

S. John Calise is supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number 2T90DE021990-06. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study. All patients provided written informed consent to receive IFN/RBV/TPV and permission for use of their medical records for this study.

Human and animal rights

The study conforms to the Institutional Review Board requirements in all institutions. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Animals were not used in this study.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2016

Autoimmunity Highlights 1/2016 Zur Ausgabe

Neu im Fachgebiet Innere Medizin

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Innere Medizin und bleiben Sie gut informiert – ganz bequem per eMail.

© Springer Medizin 

Bildnachweise