Background
Uyghur Name | Latin name | Family | Part used |
---|---|---|---|
Pirsiyavxan |
Adiantum capillus-veneris L.
| Adiantaceae | Whole plant |
Kök tantak |
Alhagi pseudalhagi (Bieb.) Desv.
| Fabaceae | Branch secretion |
Gavziban |
Anchusa italica Retz.
| Boraginaceae | Whole plant |
Serbistan |
Cordia dichotoma G.Forst.
| Boraginaceae | Fruit |
Yalmankülak |
Euphorbia humifusa Willd. Euphorbia maculata L.
| Euphorbiaceae | Whole plant |
Arpabidiyan |
Foeniculum vulgare Mill.
| Apiaceae | Fruit |
Qüqük buya |
Glycyrrhiza uralensis Fisch. ex DC.
| Fabaceae | Radix or rhizoma |
Üstihuddus |
Lavandula angustifolia Mill.
| Lamiaceae | Aerial parts |
Badrenjiboye hindi |
Melissa officinalis L.
| Lamiaceae | Whole plant |
Qilan |
Ziziphus jujuba Mill.
| Rhamnaceae | Fruit |
Glycyrrhiza inflata Batalin
| Fabaceae | Radix or rhizoma | |
Glycyrrhiza glabra L.
| Fabaceae | Radix or rhizoma |
Methods
Animals and cell lines
Laboratory instruments and reagents
Animal grouping
Construction of the U27 tumor mouse model
Sample preparation
Determination of the tumor inhibition rate and the spleen, thymus, liver, and kidney indexes
Preparation and HE staining of the U27 tumor tissue histological sections
TGF-β1 and TNF-α protein expression in the U27 tumor tissues
Data processing and analysis
Results
Effects of ASMq on the thymic, splenic, hepatic, and renal weights of the U27 tumor mice
Group | Dosage/(g/kg) | Thymus weight | Spleen weight | Liver weight | Kidney weight |
---|---|---|---|---|---|
Normal control group | - | 127.270 ± 3.382 | 187.160 ± 5.192 | 1788.910 ± 47.413 | 462.210 ± 29.221 |
U27 Tumot Model group | - | 112.369 ± 4.029* | 166.252 ± 8.568* | 1713.961 ± 49.607 | 440.741 ± 18.023* |
CTX group | 0.3 | 74.296 ± 13.993*△ | 99.052 ± 23.571*△ | 1373.506 ± 134.165*△ | 401.114 ± 15.965*△ |
ASMq.L group | 2 | 120.720 ± 6.131△▲ | 174.750 ± 10.242*△▲ | 1760.940 ± 141.710*△▲ | 434.980 ± 18.858*▲ |
ASMq.M group | 4 | 128.900 ± 4.822△▲ | 199.030 ± 9.627*△▲ | 1905.760 ± 79.073*△▲ | 440.190 ± 22.025*▲ |
ASMq.H group | 8 | 136.640 ± 7.524*△▲ | 187.810 ± 11.047△▲ | 1991.600 ± 167.884*△▲ | 448.740 ± 22.692▲ |
Effects of ASMq on the tumor inhibition rates and vital organ indexes of the U27 tumor mice
Group | Dosage/(g/kg) | Thymus index | Spleen index | Liver index | Kidney index |
---|---|---|---|---|---|
Normal control group | - | 4.032 ± 0.104 | 5.929 ± 0.153 | 56.676 ± 1.570 | 14.637 ± 0.813 |
U27 Tumot Model group | - | 3.578 ± 0.134* | 5.288 ± 0.113* | 54.585 ± 2.045* | 14.034 ± 0.619 |
CTX group | 0.3 | 2.702 ± 0.360*△ | 3.590 ± 0.599*△ | 50.088 ± 2.003*△ | 14.685 ± 0.812△ |
ASMq.L group | 2 | 3.641 ± 0.230*△▲ | 5.262 ± 0.189*△▲ | 52.956 ± 1.934▲ | 13.122 ± 0.815*△▲ |
ASMq.M group | 4 | 3.749 ± 0.203*△▲ | 5.780 ± 0.167*△▲ | 55.393 ± 2.520▲ | 12.802 ± 0.797*△▲ |
ASMq.H group | 8 | 3.712 ± 0.156*▲ | 5.100 ± 0.135*△▲ | 54.026 ± 2.271*▲ | 12.196 ± 0.503*△▲ |
Group | Dosage/(g/kg) | Tumor weight/mg | Tumor inhibition rate/% |
---|---|---|---|
Normal control group | - | 0.000 ± 0.000 | - |
U27 Tumot Model group | - | 2102.891 ± 209.690 | - |
CTX group | 0.3 | 584.273 ± 507.625△ | 72.21 |
ASMq.L group | 2 | 1445.350 ± 327.236△▲ | 31.27 |
ASMq.M group | 4 | 829.930 ± 283.190△▲ | 60.53 |
ASMq.H group | 8 | 1010.540 ± 179.564△▲ | 51.94 |
Effects of ASMq on the histological sections of the U27 tumor mice
Effects of ASMq on TGF-β1 and TNF-α protein expression in the tumor tissues of the U27 model mice
Group | Dosage/(g/kg) | TGF-β1 | TNF-α |
---|---|---|---|
U27 Tumot Model group | - | 1.4325 ± 0.1715 | 0.6783 ± 0.0964 |
CTX group | 0.3 | 1.0561 ± 0.1185△ | 0.3370 ± 0.0377△ |
ASMq.L group | 2 | 0.8537 ± 0.1090△▲ | 0.6948 ± 0.0711▲ |
ASMq.M group | 4 | 0.5770 ± 0.0889△▲ | 1.2140 ± 0.1722△▲ |
ASMq.H group | 8 | 0.5976 ± 0.0864△▲ | 1.0199 ± 0.1093△▲ |