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Acta Neurochirurgica

Erschienen in:

01.12.2009 | Experimental Research

Apoptotic effect of sonodynamic therapy mediated by hematoporphyrin monomethyl ether on C6 glioma cells in vitro

verfasst von: Shaochun Dai, Shaoshan Hu, Changjun Wu

Erschienen in: Acta Neurochirurgica | Ausgabe 12/2009

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Abstract

Purpose

Sonodynamic therapy (SDT) is a new method for treating cancer by inducing cell necrosis and/or apoptosis. However, the molecular mechanisms of cell apoptosis after SDT treatment remain unclear. In this study we investigated the apoptotic effect and mechanisms of SDT mediated by hematoporphyrin monomethyl ether (HMME) on C6 glioma cells in vitro.

Methods

The time of ultrasound irradiation was optimized by MTT. The apoptotic rate, generation of ROS (reactive oxygen species), and mitochondrial membrane potential (MMP) were detected by flow cytometry, and expressions of apoptosis proteins were measured by Western blot after SDT treatment.

Results

The results displayed that treatment of C6 glioma cells with SDT resulted in the occurrence of apoptosis, which was associated with the production of ROS and loss of MMP. The results also showed that protein expression of Bax, caspase-9, and caspase-3 significantly increased, protein expression of Bcl-2 and Fas-L significantly decreased, and Fas protein expression was unchanged after SDT treatment.

Conclusions

Our results suggested that the mitochondrial signal pathway may play a pivotal role in the apoptosis induced by SDT in C6 glioma cells.

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Titel: Apoptotic effect of sonodynamic therapy mediated by hematoporphyrin monomethyl ether on C6 glioma cells in vitro
verfasst von: Shaochun Dai
Shaoshan Hu
Changjun Wu
Publikationsdatum: 01.12.2009
Verlag: Springer Vienna
Erschienen in: Acta Neurochirurgica / Ausgabe 12/2009
Print ISSN: 0001-6268
Elektronische ISSN: 0942-0940
DOI: https://doi.org/10.1007/s00701-009-0456-5

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Abstract

Purpose

Methods

Results

Conclusions

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