Introduction
Materials and methods
Study design
Data sources
Variable | ID | Sample size | Number of SNPs | Consortium | Population | Sex | Year |
---|---|---|---|---|---|---|---|
Waist circumference | ukb-a-382 | 336,639 | 10,894,596 | Neale Lab | European | Males and Females | 2017 |
Waist-to-hip ratio | ieu-a-79 | 210,082 | 2,542,432 | GIANT | European | Males and Females | 2015 |
Testosterone levels | ebi-a-GCST90012102 | 188,507 | 16,139,906 | - | European | Males and Females | 2020 |
MAFLD | finn-b-NAFLD | 218,792 | 16,380,466 | - | European | Males and Females | 2021 |
Selection of instrumental variables
Statistical analysis for mendelian randomization
Heterogeneity and sensitivity test
Results
Instrumental variables
Results of two-sample MR analysis
Exposure | Number of SNPs | MR methods | outcome | OR(95%CI) | p-value |
---|---|---|---|---|---|
Waist circumference | 180 | MR Egger | MAFLD | 1.74 (0.42,7.22) | 0.447 |
WME | 3.88 (1.81,8.29) | < 0.001 | |||
IVW | 3.53 (2.23,5.57) | < 0.001 | |||
Simple mode | 3.42 (0.57,20.42) | 0.179 | |||
Weighted mode | 3.58 (1.05,12.16) | 0.043 | |||
Waist-to-hip ratio | 29 | MR Egger | 1.38 (0.04,44.83) | 0.858 | |
WME | 1.87 (0.69,5.12) | 0.22 | |||
IVW | 2.29 (1.12,4.66) | 0.022 | |||
Simple mode | 2.56 (0.39,16.62) | 0.333 | |||
Weighted mode | 2.39 (0.495,11.49) | 0.288 | |||
Testosterone level | 135 | MR Egger | 1.67 (0.77,3.62) | 0.199 | |
WME | 1.71 (0.86,3.41) | 0.125 | |||
IVW | 1.93 (1.30,2.87) | 0.001 | |||
Simple mode | 2.32 (0.59,9.09) | 0.227 | |||
Weighted mode | 1.58 (0.80,3.14) | 0.192 |
Result of heterogeneity and sensitivity test
Exposure | outcome | MR methods | p of pleiotropy | p of Cochran Q |
---|---|---|---|---|
Waist circumference | MAFLD | 0.305 | ||
MR Egger | 0.204 | |||
IVW | 0.203 | |||
Waist-to-hip ratio | 0.772 | |||
MR Egger | 0.834 | |||
IVW | 0.864 | |||
Testosterone level | 0.662 | |||
MR Egger | 0.161 | |||
IVW | 0.173 |