Background
Methods
Human sample collection and preparation
Mouse sample collection and preparation
2D-PAGE and MS identification
Evaluation of the LC-MS/MS identifications
CKs | Type I(acidic)/II(neutral or basic) | Previously described in human non-tumour-tissue | Previously described in ocular epithelium | Location1
| Total count in 102 mouse spots - % |
---|---|---|---|---|---|
Very likely endogenous (observed in <5% of human samples)
| |||||
3 | II | Cornea19,17
| B | 0% | |
4 | II | Sebaceous glands, airway epithelium19
| Conjunctiva17, cornea5
| B | 4% |
7 | II | Glands19
| Central basal cornea17No found20
| - | 0% |
8 | II | Glands19
| Cornea21, conjunctiva17
| - | 0% |
11 | I | Epidermis19
| - | 0% | |
12 | I | Cornea19,2
| C | 0% | |
13 | I | Glands, airway epithelium19,2
| Conjuntiva25, cornea5
| B | 2% |
15 | I | Basal keratinocytes2
| Limbal basal7
| L | 2% |
17 | I | Hair follicle, nails2
| Cornea17
| - | 0% |
18 | I | Glands, simple epithelium19,2
| Cornea21
| - | 0% |
19 | I | Glands, airway epithelium19
| Conjunctiva4, basal limbus2
| L | 0% |
20 | I | Gastrointestinal tract epithelium2
| - | 0% | |
23 | I | Non (found in pancreatic tumours)22
| - | 0% | |
Uncertain endogenous (observed in >5% of human samples)
| |||||
1 | II | Epidermis19
| B | 36% | |
2e | II | Epidermis19
| B | 78% | |
5 | II | Epidermis, glands, airway epithelium, hair follicles19
| Conjuntiva25, cornea19, 24
| B | 3% |
6A | II | Glands, airway epithelium, hair follicles19
| B | 27% | |
9 | I | Palmoplantar epidermis2
| B | 99% | |
10 | I | Epidermis19
| B | 75% | |
14 | I | Epidermis, glands, airway epithelium, hair follicles19
| Conjuntiva25, cornea17
| B | 18% |
16 | I | Epidermis19
| Cornea4
| B | 6% |
Immunohistochemistry
Statistical analyses
Results and Discussion
CKs | Spot number in gels (6%=',12%=*) | UniProtKB/Swiss-Prot | Observed M (kDA) | Theoretical M (kDA) | Observed pI | Theoretical pI | Coverage (%) | Fraction of protein covered (%)1
|
---|---|---|---|---|---|---|---|---|
3 | 6503' | K2C3_HUMAN | 64 | 64.5 | 6.90 | 6.12 | 12 | 56 |
5213* | P12035 | 28 | 6.60 | 3 | 34 | |||
5610* | 60 | 7.00 | 31 | 88 | ||||
5713* | 80 | 7.05 | 9 | 56 | ||||
4 | 8402' | K2C4_HUMAN | 62 | 57.3 | 7.80 | 6.25 | 6 | 21 |
5602' | P19013 | 65 | 6.70 | 4 | 57 | |||
5610* | 60 | 7.00 | 3 | 24 | ||||
12 | 0201' | K1C12_HUMAN Q99456 | 51 | 53,4 | 4.30 | 4.70 | 9 | 40 |
13 | 0301' | K1C13_HUMAN | 56 | 49.6 | 4.40 | 4.91 | 25 | 71 |
1205' | P13646 | 56 | 4.80 | 35 | 71 | |||
1617* | 50 | 5.05 | 16 | 64 | ||||
1618* | 50 | 5.10 | 34 | 90 | ||||
2402* | 38 | 5.50 | 5 | 19 | ||||
15 | 2201' | K1C15_HUMAN P19012 | 52 | 49.2 | 5.60 | 4.71 | 16 | 78 |
19 | 2513* | K1C19_HUMAN | 39 | 44.1 | 5.40 | 5.04 | 23 | 81 |
1517* | P08727 | 40 | 5.30 | 17 | 81 |