Our study is the largest case series of patients with TCA at a single center to evaluate the diagnostic performance of preoperative barium enema [
7,
24]. The major advantages of patient enrollment in our study are as follows. First, enrollment spanned from 2007 to 2019, during which radiologic contrast enema was well recognized and specifically applied for HD [
25,
26]. Second, only patients who did not undergo any surgical treatment prior to barium enema were observed in our study. This potentially reduced confounding errors due to surgical interference by the bowel. Finally, all examinations were conducted in a single center, and the operating supplies, techniques and diagnostic algorithms were consistent for all patients. More importantly, all available radiographic signs regarding HD and TCA were included in our study to evaluate the overall abdominal condition of the patient.
We evaluated the diagnostic accuracy of preoperative barium enema and demonstrated that the radiographic signs used to diagnose “segmental” HD, including rectosigmoid index, transition zone, irregular contraction, gas-filled small bowel and microcolon, were not reliable for diagnosing TCA. Previous studies indicated that rectosigmoid index ≤ 1 and transition zone exhibited potential value for diagnosing “segmental” HD with sensitivity values from 68–86% and 63–94%, respectively. In addition, the combination of rectosigmoid index ≤ 1 and transition zone was more useful for screening for HD [
27,
28]. However, in patients with TCA, the barium enema does not always present with these characters. Based on our results, radiologist 1 obtained a rectosigmoid index ≤ 1 in 34 patients (34/44, 86%) with TCA, which was twice the value obtained by radiologist 2 (17/44, 39%). When the whole colon and rectum are aganglionic and spasmodic, rectal ampulla may be difficult to identify in barium enema, even for experienced radiologists. Different radiologists may choose different radiographs to record rectosigmoid index ≤ 1 in a patient, which would influence judgment. Transition zone is regarded as an effective radiographic sign to identify the extent of aganglionic bowel in “segmental” HD and could therefore facilitate surgical planning [
21]. In our study, the two radiologists noted these occurrences in 12 (12/44, 27%) and 26 (26/44, 59%) patients, and both of these values were higher than that reported in a previous study of TCA (2/17, 12%) [
7]. However, transition zone was still mostly found in the transverse and the ascending colon, which is consistent with previous studies demonstrating that the pathologic extent of aganglionic bowel is more proximal than the site of the transition zone [
7,
29]. This finding may be related to the patients receiving conservative treatment, which creates high pressure in the proximal aganglionic colon and can mislead surgical planning [
30]. Rectosigmoid index ≤ 1 and transition zone appear to be more accurate in “segmental” HD compared with TCA [
30,
31]. Irregular contraction was previously found in approximately 50% of patients with HD and TCA, a finding that was confirmed by our results but showed fair agreement among radiologists [
15,
25]. Gas-filled small bowel and microcolon are often simultaneously identified at the time of barium examination in most TCA patients, both neonatal and non-neonatal, who suffered from chronic defecation difficulties [
15]. Our results consistent with a recent study by Shan Zheng et al., but good diagnostic consistency was not noted among different radiologists [
32]. For microcolon, there is no reported standard measurement available for different ages, particularly in newborns, given the lack of consistency in the X-ray findings, thus limiting the application of diagnosis, which is consistent with our study [
7,
33].
Question-mark-shaped colon was first described by Sane and Girdany in 1973 but only a small number of patients with TCA exhibit typical question-mark-shaped colon [
16,
17]. Ileocecal valve reflux was first systematically described by Chandler in 1970 but was found to be a nonspecific finding in newborns, in which it also depended on the amount and pressure of the rectal contrast agent used during the enema [
7,
18]. Although question-mark-shaped colon and ileocecal valve reflux were not particularly sensitive, the 2 radiologists in our study showed moderate agreement in the accuracy of question-mark-shaped colon and ileocecal valve reflux in patients with TCA, indicating the potential diagnostic performance. Therefore, we further explored the value of the question-mark-shaped colon and ileocecal valve reflux identification in neonatal and non-neonatal patients, even in patients with TCA extension to the ileum. Our results showed that ileocecal valve reflux was a reliable radiographic sign in preoperative barium enema for both neonatal and non-neonatal patients with TCA. Although poor consistency was noted in diagnosis between question-mark-shaped colon and ileocecal valve reflux identification between individual radiologists, the combination of question-mark-shaped colon and ileocecal valve reflux identification in both radiologists could increase accuracy by up to 75%. This finding may help clinicians perform accurate intraoperative multiple full-thickness punch biopsy and develop appropriate medical treatments [
12,
14].
Our study had some limitations. First, because our study design was retrospective, there is a potential risk of selection bias. Second, these analyses were performed using collected barium enema images, which prevented a dynamic analysis and potentially influenced the radiological interpretation. Third, a control group comprising children without HD diagnosis or children with HD without TCA was not included. Therefore, we suggest that this issue should be further studied. Finally, the number of patients included in our study was limited to 44 patients. Multicenter studies about preoperative barium enema involving more patients with TCA are expected to be performed in the future.