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Journal of Cardiovascular Translational Research

Erschienen in:

01.06.2013

Beneficial Cardiac Effects of Caloric Restriction Are Lost with Age in a Murine Model of Obesity

verfasst von: Majd AlGhatrif, Vabren L. Watts, Xiaolin Niu, Marc Halushka, Karen L. Miller, Konrad Vandegaer, Djahida Bedja, Karen Fox-Talbot, Alicja Bielawska, Kathleen L. Gabrielson, Lili A. Barouch

Erschienen in: Journal of Cardiovascular Translational Research | Ausgabe 3/2013

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Abstract

Obesity is associated with increased diastolic stiffness and myocardial steatosis and dysfunction. The impact of aging on the protective effects of caloric restriction (CR) is not clear. We studied 2-month (younger) and 6–7-month (older)-old ob/ob mice and age-matched C57BL/6J controls (WT). Ob/ob mice were assigned to diet ad libitum or CR for 4 weeks. We performed echocardiograms, myocardial triglyceride assays, Oil Red O staining, and measured free fatty acids, superoxide, NOS activity, ceramide levels, and Western blots. In younger mice, CR restored diastolic function, reversed myocardial steatosis, and upregulated Akt phosphorylation. None of these changes was observed in the older mice; however, CR decreased oxidative stress and normalized NOS activity in these animals. Interestingly, myocardial steatosis was not associated with increased ceramide, but CR altered the composition of ceramides. In this model of obesity, aging attenuates the benefits of CR on myocardial structure and function.

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Titel: Beneficial Cardiac Effects of Caloric Restriction Are Lost with Age in a Murine Model of Obesity
verfasst von: Majd AlGhatrif
Vabren L. Watts
Xiaolin Niu
Marc Halushka
Karen L. Miller
Konrad Vandegaer
Djahida Bedja
Karen Fox-Talbot
Alicja Bielawska
Kathleen L. Gabrielson
Lili A. Barouch
Publikationsdatum: 01.06.2013
Verlag: Springer US
Erschienen in: Journal of Cardiovascular Translational Research / Ausgabe 3/2013
Print ISSN: 1937-5387
Elektronische ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-013-9453-4

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