Background
Colorectal cancer (CRC) is the third most common cancer type globally with an annual incidence of over 1.2 million new cases. It is also a leading cause of cancer-related mortality, and accounts for >600,000 cancer-related deaths each year [
1‐
3]. Traditionally, CRC has been classified based on standardized histopathological features; more recently, molecular genotyping has also been investigated. Both technologies revealed a complex and heterogeneous nature of CRC. Several pathological mechanisms and signalling pathways have been identified as contributing to the pathophysiology of this disease, and some of these mechanisms already operate in the precancerous lesions, which has been termed the common serrated tumour pathway [
4]. Among these signalling pathways, bone morphogenetic protein signalling is relevant [
5].
Bone morphogenetic proteins (BMP) belong to the transforming growth factor beta superfamily (TGFβ) and are important regulators of embryogenesis, and patterning of body axes. They regulate adult tissue homeostasis through control of cell growth, apoptosis and differentiation. The biological effects of BMPs have been mostly studied in mesoderm-derived cells and tissues, and to lesser degree in epithelial cells and tissues. A number of studies have examined the association of abnormal BMP signalling with cancer initiation and/or progression. Changes in BMP signaling via the Smad cascade has been associated with a number of tumour types [
6,
7]. Also, BMP-2, -4 and -7 have exhibited altered expression in many types of malignancies including breast, prostate, osteosarcoma, glioma, ovarian, pancreatic, lung, and other cancers.
Dysfunctional BMP signaling has been implicated in CRC. Mutations in BMP receptor 1A (
BMPR1A) or its downstream effector SMAD4 have been identified in patients with juvenile polyposis (JP), a rare autosomal dominant hamartomatous polyposis syndrome with a 12 fold increased lifetime risk for development of CRC [
5]. BMP signaling molecules are expressed in human CRCs, and BMP signaling has been shown to induce growth suppression through the BMPR1A and intracellular SMAD pathway [
8‐
10].
We have previously reported the global mRNA expression profiling in CRC tissues as compared to adjacent normal mucosa, and identified several dysregulated mRNAs including down regulation of BMP2 [
2]. In the current study, we examined the biological role of BMP2 in CRC. We employed lentiviral gene transfer technology to overexpress BMP2 in the human HCT116 CRC cell line, and examined its effects on cell proliferation, migration, clonogenic potential, drug resistance, and in vivo tumour growth.
Methods
Cells lines and tissue culture
The human colorectal cancer (HCT116) cell line was obtained and subsequently was authenticated by Genetica DNA Laboratories, Inc. Burlington, (NC, USA). Cells were maintained in DMEM supplemented with 10 % fetal bovine serum (Gibco-Invitrogen, Waltham, MA, USA) and 100 mg/l penicillin/streptomycin. All cells were maintained in a 37 °C incubator with humidified 5 % CO2.
Lentiviral transduction
Lentiviral particles encoding for LV BMP2 (LP-A0241-Lv105-0200-P) or control lentiviral particles (LP-EGFP-LV105-0200) were purchased from Genecopoeia (Genecopoeia Inc., Rockville, MD, USA). One hundred fifty thousand HCT116 cells were seeded in complete DMEM in 24-well plate. Twenty-four hours later (~80 confluency), media was removed and then 20 μl of crude lentiviral particles in 500 μl of DMEM + 5 % heat-inactivated serum (Invitrogen) and 1 % Pen–Strep supplemented with polybrene (8 μg/ml; Sigma, St. Louis, MO, USA) was added to the cells. Seventy-two hours later, media was removed and transduced cells were selected with puromycin (1 μg/ml, Sigma, St. Louis, MO. USA) for 1 week or until stably transduced cells were generated.
Gene expression microarray
RNA isolation, gene and microRNA expression analysis were performed in accordance with our previously published protocols [
11]. In brief, RNA was isolated using Total RNA Purification Kit from Norgen-Biotek Corp. (Thorold, ON, Canada) and were quantified using NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA). Total RNA was labelled and then hybridized to the Agilent Human SurePrint G3 Human GE 8 × 60 k mRNA microarray chip (Agilent Technologies). All microarray experiments were conducted at the Microarray Core Facility (Stem Cell Unit, College of Medicine, King Saud University). Data were subsequently normalized and analyzed using GeneSpring 13.0 software (Agilent Technologies). Pathway analyses were conducted using the Single Experiment Pathway analysis feature in GeneSpring 13.0 (Agilent Technologies). The Benjamini-Hochberg false discovery rate (FDR) multiple testing correction method [p(corr) < 0.02] was utilized and two fold cut-off were used to enrich for significantly changed transcripts.
mRNA validation by qRT-PCR
mRNAs expression levels were validated in LV control and LV BMP2 HCT116 cells using SYBR Green-based qRT-PCR and the Applied Biosystems ViiA™ 7 Real-Time PCR System. Two μg of total RNA was reverse transcribed using High Capacity cDNA Reverse Transcript Kit (Part No: 4368814; ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol. Primer sequences used in the current study are listed in Table
1. The relative expression level was calculated using −ΔΔCT. GAPDH was used as an endogenous control.
Table 1
List of CYBR green primers used in current study
1 | CASP10 | F 5′ GAAGCCTTACCGCAGGAGTC 3′ |
R 5′ GTGCACCATTTGTGGCTCTG 3′ |
2 | HDAC4 | F 5′ ACTGGTACGGGAAAACGCAG 3′ |
R 5′ TTTGGCGTCGTACATTCCCA 3′ |
3 | MAPK9 | F 5′ TGGGCTACAAAGAGAACGTTGAT 3′ |
R 5′ AAGGTCGCGGGGAAGGATA 3′ |
4 | MDM2 | F 5′ AGGAGATTTGTTTGGCGTGC 3′ |
R 5′ TGAGTCCGATGATTCCTGCTG 3′ |
5 | WNT7A | F 5′ ATGCCCGGACTCTCATGAAC 3′ |
R 5′ GTTCTCCTCCAGGATCTTTCGG 3′ |
6 | WNT3 | F 5′ GGACAAAGCTACCAGGGAGT 3′ |
R 5′ CTGCACATGAGCGTGTCACT 3′ |
7 | WNT4 | F 5′ CATGAGTCCCCGCTCGTG 3′ |
R 5′ CCAGGTACAGCCAGTTGCTC 3′ |
8 | SMAD4 | F 5′ GGATACGTGGACCCTTCTGG 3′ |
R 5′ TGTGCAACCTTGCTCTCTCAA 3′ |
9 | TERT | F 5′ GGCACGGCTTTTGTTCAGAT 3′ |
R 5′ TCCGGGCATAGCTGGAGTAG 3′ |
10 | AFT3 | F 5′ GTGAGTCCTCGGTGCTCG 3′ |
R 5′ GCATCATTTTGCTCCAGGCT 3′ |
11 | HOMEZ | F 5′ CTGGACTGCGCTATCTCTGAAG 3′ |
R 5′ TGAACTACTGAGACCGCTGG 3 |
12 | FADD | F 5′ GGGAAGAAGACCTGTGTGCAG 3′ |
R 5′ GAGCCAGCCTTCTCCAATCT 3′ |
13 | BCL2 | F 5′ AGATTGATGGGATCGTTGCCT 3′ |
R 5′ AGTCTACTTCCTCTGTGATGTTGT 3′ |
14 | BIRC5 | F 5′ AGCCAAGAACAAAATTGCAAAGG 3′ |
R 5′ CGCACTTTCTCCGCAGTTTC 3′ |
15 | E2F1 | F 5′ AACTGACCATCAGTACCTGGC 3′ |
R 5′ GGGATTTCACACCTTTTCCTGG 3′ |
16 | E2F2 | F 5′ AGGAGCAGACAGTGATTGCC 3′ |
R 5′ GGTTGTCCTCAGTCCTGTCG 3′ |
17 | BMP2 | F 5′ GGAACGGACATTCGGTCCTT 3′ |
R 5′ CACCATGGTCGACCTTTAGGA 3′ |
18 | GAPDH | F 5′ CTGGTAAAGTGGATATTGTTGCCAT 3′ |
R 5′ TGGAATCATATTGGAACATGTAAACC 3′ |
Measurement of cell viability, clonogenic and sphere formation
The viability of LV control and LV BMP2 HCT116 cells was determined using alamarBlue assay as previously described [
12]. All assays were carried out with appropriate controls. Briefly, 10,000 cells were cultured in a 96-well plate and cell viability was measured at the indicated time points by adding 10 % volume alamarBlue assay reagent and measuring absorbance at 570 λ. The colony forming ability of HCT116 cells transduced with BMP2 was determined using clonogenic assay as previously described [
12,
13]. Briefly, LV control or BMP2 HCT116 cells were seeded in 12-well plates in different serial dilution (1:2–1:64). Initial seeding density was 0.015 × 10
6 cells per well, and incubated at 37 °C under 5 % CO
2 for 10 days. The plates were then washed and stained with Diff-Quik stain set (Siemens Healthcare Diagnostics), and the plates were scanned and number of colonies were observed under microscope. The fraction of surviving cells was estimated by comparing BMP2 to LV control cells. The experiment was done twice in duplicate. Furthermore, the clonogenic assay was conducted to examine the effect of 5-fluorouracil on cell growth and proliferation in both cells. A total of 1 × 10
6 cells were seeded in T25 flask. After 48 h of exposure to 1.5 µM of 5-fluorouracil, the cells were trypsinized and reseeded in 12-well plates as described above to observe the effect of the drug.
Multicellular tumor spheroids were produced in 60 mm low cell binding dishes (Nunc; ThermoFisher Scientific). LV control or BMP2 HCT116 cells were trypsinized from monolayers and transferred to the low cell binding dishes. The formation of tumor spheroids was performed with 10,000 cells. On day 10, established spheroids were analysed.
Cell migration and proliferation
Real-time measurement of LV control and BMP2 HCT116 cell migration and proliferation was executed using the xCELLigence RTCA DP device (ACEA Biosciences, San Diego, CA). For migration study, cells were starved for 24 h in 1 % serum media, followed by seeding 0.08 × 106 cells per well in 16-well microelectronic sensor plate pre-coated with fibronectin (1:500), two chamber trans-well plates (CIM-plate insert; ACEA Bioscience) containing the respective serum conditions. Medium containing 10 % serum (chemo-attractant) and 1 % serum (negative control) was added to the bottom wells.
For proliferation assay, cells were seeded (0.04 × 106 cells/well) in two chamber plates (E-plate insert; ACEA Bioscience). Proliferation and migration of cells was measured from the interaction of cells with the electrodes on the bottom surface of top chamber and represented as a change in cell index (CI). The electrical impedance was captured every 15 min for an experimental duration of ~68 h. The rate of migration and proliferation is expressed as the CI or the change in electrical impedance at each time-point. The Cell Index at each time point is defined as (Rn-Rb)/4.6, where Rn is the cell-electrode impedance of the well when it contains cells and Rb is the background impedance of the well with the media alone. Values are expressed as the mean ± SEM of the 3 replica wells.
For conventional migration, the BD transwell migration system with 8 µ pore size was utilized as previously described [
14]. Inserts were placed in a 24-well plate, and 1.56 × 10
5 cells in 1 % serum were added to the top of the chamber, and 10 % serum added to the bottom chamber. Hundred hours later, inserts were fixed and stained with SIEMENNS DIFF-QUICK stain set (Siemens Healthcare Diagnostics), and the number of migrating cells was counted using a light microscope.
Measurement of apoptosis
Fluorescence-based apoptosis was determined in cells after exposure to different concentration of 5-fluorouracil, using acridine orange and ethidium bromide (AO/Etbr) staining method. After treatment, the LV control and BMP2 HCT116 cells were stained with dual fluorescent staining solution (1 µl) containing 100 µg/ml AO and 100 µg/ml EB (AO/EB, Sigma, St. Louis, MO). Cells were washed twice with PBS and were gently mixed with AO/EB (1:100) dye solution for 1 min; afterwards, the cells were observed and photographed under a Nikon Eclipse Ti fluorescence microscope. Cells cultured without drug were considered as experiment control. Acridine Orange/Ethidium Bromide Staining uses combination of two dyes to visualize cells with aberrant chromatin organization. The differential uptake of AO/EB allows the identification of viable and non-viable cells. Particularly, Acridine Orange was used to visualize the number of cells which has undergone apoptosis.
In vivo tumorigenicity assay in SCID mice
In vivo tumor formation was carried out as previously described [
15]. Briefly, 6- to 8-week-old severe combined immunodeficient (SCID) mice were utilized for the xenograft experiments. Ten million LV control or LV BMP2 HCT116 cells were suspended in PBS and subcutaneously injected into the right flank of SCID mice. Tumor volume was measured twice weekly using a caliper, and tumor volume was calculated as (tumor length × width
2)/2. At the end of the experiment, tumors were excised, fixed in 10 % buffered formalin, and embedded in paraffin and sectioned. Sections were the stained with haematoxylin and eosin.
Immunohistochemistry
We used commercially available anti-bcl-2 (124) mouse monoclonal primary antibody (790-4464; Ventana; Roche) and anti-Ki67 antibody rabbit polyclonal (ab15580; Abcam) in 1:200 dilution to stain the tumor section. Four-micron-thick sections were immunostained for Bcl-2 and Ki67 protein using the immunohistochemical (IHC) staining protocol optimized in King Khalid university hospital, King Saud University, Riyadh. The IHC staining was carried out using a BenchMark XT fully automated IHC/ISH staining instrument (Ventana Medical system Inc, Tucson, Arizona, USA), as per manufacturer’s protocol using proprietary reagents. Briefly, slides were deparaffinized on the automated system with EZ Prep solution (Ventana). Enzymatic retrieval was used with protease 1 solution (Ventana). The detection system used was the ultraView Universal DAB Detection Kit (760-500; Ventana; Roche), and slides were then counterstained with hematoxylin and mounted.
Statistical analysis
Statistical analyses and graphing were performed using Microsoft Excel 2010 and GraphPad Prism 6.0 software (GraphPad, San Diego, CA, USA). P values were calculated using the two-tailed t test.
Discussion
Identifying signalling pathways that are associated with cancer is important in order to identify novel targets for therapy. In the current study, we have demonstrated that enhanced expression of BMP2 in CRC cells reduced their growth, enhanced apoptosis and decreased tumor development in vivo. Given our previous demonstration that CRCs expressed lower levels of BMP2 [
2]; the more pronounced downregulation of BMP2 in metastatic-recurrent patients from the GSE21510, combined with the current data, this all indicate that BMP2 and BMP signalling are important regulators of CRC development and progression. Although the exact molecular mechanism leading to BMP2 downregulation in CRC is still unknown, genomic deletion, DNA methylation or epigenetic regulation could potentially play a role in BMP2 downregulation in CRC. Downregulation of BMP2 was also observed in several CRC cell lines (Additional file
5: Figure S4).
We employed HCT116 as a cell model for CRC; overexpression of BMP2 inhibited CRC proliferation, migration, colony and spheroid formation and in vivo tumor growth, demonstrating a tumor suppressive role for BMP2. Restitution of BMP2 expression also restored sensitivity to 5-FU. Molecular signature of HCT116 cells overexpressing BMP2 revealed multiple altered genetic pathways including cell cycle, DNA replication, DNA damage response, and WNT signalling, which could provide the biological explanations for the observed association between BMP2 status and CRC. Interestingly, our recent data revealed a significant role for TGF-beta in CRC, as pharmacological inhibition of TGF-beta pathway inhibited CRC growth in vitro [
2]. Similar to our findings, Zhang et al. also recently reported an inhibitory role for BMP2 in governing the proliferation and aggressive behavior of human CRC cells [
16].
Genetic studies showed mutations in the TGF-β signaling pathway to increase the mortality risk in CRC patients [
17]. Additionally, a number of other studies have reported that BMP2 expression is dysregulated in CRC. BMPR1a, BMPR1b, BMPR2, phosphorylated Smad1, and Smad4 are expressed in mature colon cells in normal adult human and mouse colon [
8]. Several members of the BMP family (BMP2, BMP3 or BMP7) are downregulated in CRC, and have been shown to promote apoptosis, differentiation, and inhibit proliferation [
8‐
10,
16]. Our study corroborates these findings; identifying a specific role for BMP2 in CRC. Furthermore, we provide evidence suggesting that targeting BMP2 signalling could be a possible approach to limit CRC tumour growth.
While our study suggest a suppressive role for BMP2 in CRC, the precise role for the BMP signalling in tumour development is controversial since it has been reported to either promote or inhibit tumorogenesis [
7]. Therefore it is possible that the effect of BMP signalling is dependent on tumour type as well as the status of other pathways in different cancers. For example, Alarmo et al. reported that BMP7 can either promote or inhibit carcinogenesis, depending on dose, type of cell or tissue origin, and the environmental niche, reflecting the complexity of networks and pathways involved in cancer development and progression [
18].
Conclusions
We propose that loss of BMP2 leads to deregulation in multiple signaling pathways involving cell cycle, DNA replication, and DNA damage response resulting in CRC development, progression and possibly resistance to chemotherapy. Targeting the BMP2 pathway may therefore offer potential therapeutic benefit in CRC therapy.
Authors’ contributions
RV carried out experiments and helped to draft the manuscript; SY performed in vivo experiments, MA conceived the study, and participated in its design and coordination and helped to draft the manuscript; MK conceived the study, and participated in its design and helped to edit the manuscript; FFL conceived the study, and participated in the in vivo experiment design and edited the manuscript; AA conceived the study, and participated in its design and coordination and helped to draft the manuscript; NMA obtained funding, conceived the study, and participated in its design and coordination, drafted the manuscript. All authors read and approved the final manuscript.
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