Background
Globally, oral cancer is a huge threat to our health. Approximately 300,400 people were diagnosed and 145,400 died of oral cancer in a year [
1]. Among all types of oral cancer, squamous cell carcinoma (SCC) accounts for more than 95%. Derived from stratified squamous epithelium of the oral mucosa, SCC primarily occurred at the buccal mucosa and tongue [
2]. Tobacco, alcohol use, radiation and human papillary virus infection are implicated as risk factors for SCC [
3].
Tongue squamous cell carcinoma (TSCC) is a special type of oral cancer. Patients may present with non-healing ulcers and pain in the mouth. TSCC usually grows as infiltrative and/or exophytic lesions and presents with local invasion. Dysarthria during the development of the disease implies the invasion of the tumor into the deep muscles. TSCC do not often have distant metastasis, but cervical lymph node relapse may occur in a large percentage of TSCC patients, which usually indicates poor prognosis. Analysis concerning the risk factors for cervical lymph node relapse is thus critical for clarifying the onset and progression of TSCC as well as improving the prognosis of patients. Few studies have been focusing on the clinical and pathological factors related to the prognosis of TSCC [
4]. Yet few reports investigated the pathological features of TSCC.
It was established that tumor cells at the invasive front differed substantially from the central and superficial tumor cells. The invasive front of tumor often indicated the aggressiveness of TSCC [
5]. In addition, the depth of invasion may also reflect the progression of tumor. Moreover, it was reported in esophageal tumors that the invasive front acted as predictive factors for metastasis and prognosis [
6]. In this cohort study, using both univariate and multivariate analyses, we focused on the predictive value of the pathological features on cervical lymph node relapse and TSCC prognosis.
Methods
Subjects
Subjects involved in this study were recruited from the outpatient clinic at Shanghai Jiao Tong University School of Medicine. All patients were pathologically confirmed as TSCC by having biopsy before surgery and then staged as T
1–2 with no cervical lymph node relapse (N
0). The TNM stage was evaluated by the clinical check and with the help of CT and B-ultrasound. TSCC samples were collected following tumor resection. No radiotherapy or chemotherapy had been performed before the collection of tumor samples. Baseline information including gender, age, pathological features of tumor, location of tumor (anterior, middle and posterior), habits of alcohol consumption and smoking, and treatment (neck dissection vs. observation) was documented. Pathological features of TSCC samples included the growth pattern of the invasive front (crumb vs. trabes), depth of invasion (< 4 mm vs. ≥4 mm), grade of pathological differentiation (well vs. Moderate/poor), and neurovascular invasion (yes vs. no). The growth pattern of the invasive front was determined by the pathologists at the hospital according to Rivera et al. [
7]. This study was performed following the protocols approved by the Ethical Committee of Shanghai Jiao Tong University School of Medicine. An informed consent was signed by all participants in this clinical study.
Therapy
Patients enrolled in the current study were randomly divided into neck dissection group and observation group. An elective supraomohyoid neck dissection was performed for patients in the neck dissection group. In cases with TSCC recurrence and cervical lymph node relapse during the follow-up, a corresponding therapy was applied to patients according to their condition. Radical resection, chemotherapy and radiotherapy might be included.
Follow-up
The maximum follow-up duration was 60 months. During the follow-up period, patients were examined for tumor recurrence and cervical lymph node relapse every 3 months during the first 24 months, then every 6 months for the following 36 months. Examination included clinical inspection and CT or MRI. All nodal relapse patients were pathologically proven by surgical pathology.
Data analysis
In this study, we focused on the association between the clinicopathological features of TSCC and patient prognosis (disease free survival), in addition to collecting both the Disease specific survival (DSS) and the Disease free survival (DFS). Univariate analysis was performed with Chi-square test for cervical lymph node relapse and Kaplan-Meier survival analysis and log rank P value for patient prognosis; multivariate analysis was performed with Cox regression. Variations including gender, age, pathological features of tumor, location of tumor, habits of drinking and smoking, and treatment were involved in the growth pattern. Odd ratio (OR), hazard ratio (HR) and their corresponding 95% confidence interval (CI) were calculated to measure the association. SPSS software, version 17.0 (SPSS, Chicago, IL) was used for the analysis, with P < 0.05 being significant.
Discussion
The present study investigated the correlation between the clinicopathological features, cervical lymph node relapse and TSCC prognosis. Both univariate and multivariate analyses were performed. In the univariate analysis, trabes growth pattern, depth of invasion larger than 4 mm, pattern of invasive front, poor pathological differentiation and neurovascular invasion were all considered as risk factors for cervical lymph node relapse. TSCC in the posterior tongue region and unfavorable habits of smoking and drinking were related to the higher risk of metastasis. However, in the multivariate analysis, only patients with trabes growth pattern in the invasive front and depth of invasion larger than 4 mm were found to have higher risk of metastasis. Similarly, concerning the prognosis of TSCC patients, in univariate analysis, trabes growth pattern of invasive front, depth of invasion larger than 4 mm, neurovascular invasion, habits of smoking and drinking were associated with poor TSCC prognosis. In multivariate analysis, only elder age and trabes growth pattern of invasive front were considered as predictors of poor prognosis.
As previously reported, the average age of oral cancer was 63 years old [
8]. In addition, elderly males usually suffered a higher risk of TSCC. In the current study, we observed that age was not significantly associated with cervical lymph node relapse both in univariate and multivariate analyses. However, age may serve as a critical predictor for the prognosis of TSCC. Old patients diagnosed at an age elder than 55 had significantly poorer prognosis compared with younger group.
Growth pattern of the tumor, depth of invasion, pathological differentiation and neurovascular invasion all indicated the aggressiveness of the tumor. Here in the univariate analysis, they were all proved to be risk factors for TSCC metastasis. However, some of such factors might be dependent on others. Hence a multivariate analysis was performed to clarify the independent risk factors. In the cox regression analysis, adjusted by gender, age, pathological features of tumor, location of tumor and treatment, trabes type of invasive front and depth of invasion greater than 4 mm were confirmed to be independent predictors of TSCC cervical lymph node relapse.
In the study, the growth pattern of TSCC invasive front was classified as crumb (islet-infiltrating cells with wide fronts of invasion) or trabes (thin infiltrating cords). A third pattern with individual infiltrating cells has also been reported previously in literature [
7]. However, such growth pattern was not observed in our samples. As early as in 1984, Yamamoto et al. presented that oral cavity SCC with cord-like type (i.e. trabes) of invasive front showed a higher risk of metastasis [
9], which is further confirmed with the results shown our research. Concerning the depth of tumor invasion, our results were in accordance with previous studies, which proposed a cut-off value at 5 mm of tumor depth [
10,
11]. Yet according to our results, metastasis was indicated in patients with the depth of invasion larger than 4 mm, in consistence with Ambrosch et al. [
12]. Moreover, an anatomical invasion of tumor deeper than the intrinsic tongue muscles, paralingual and sublingual spaces, and medial and lateral extrinsic tongue muscles, was a potent indicator of cervical lymph node relapse in TSCC [
13]. The effect of neurovascular invasion and histological differentiation on cervical lymph node relapse has been widely investigated in SCC [
14,
15]. In our results, neither neurovascular invasion nor histological differentiation was confirmed as independent predictors of cervical lymph node relapse in TSCC. We also found that cervical lymph node relapse is the most important prognostic factor in SCC with the highest HR value in multivariate analysis.
In the results of the current study, we also observed that neck dissection did not influence the metastasis and prognosis of TSCC. Results were contradictive concerning the effect of neck dissection on TSCC patients. Some suggested that neck dissection significantly reduces the mortality and increases the 5-year disease-free survival [
16‐
18]. Yet some proposed that neck dissection is not necessary until a node is detectable [
19]. Currently, in the treatment of TSCC, an elective neck dissection is suggested only for patients with certain risk factors, as Fakih et al. proposed in their research [
20]. Hence, for patients with risk factors including trabes invasive front and depth of tumor greater than 4 mm, an elective neck dissection is suggested to improve the prognosis of these patients.
Effects of alcohol drinking and smoking on TSCC was also evaluated in this study. Drinking and smoking have been regarded as risk factors for TSCC [
4]. According to our results, in univariate analysis, both smoking and drinking were correlated with cervical lymph node relapse and poor prognosis. However, the effect was not significant in multivariate analysis adjusted by gender, age, pathological features of tumor, location of tumor and treatment. Similarly, in the report of Wildt et al., tobacco smoking, but not alcohol consumption, was observed to have significant influences on the prognosis in the Cox proportional hazards regression analysis, with T-stage, N-stage, clinical stage, tumor size and gender of patients as adjusted variables [
21]. Moreover, it was reported that the alcohol consumption and smoking were related to the location of SCC. Tobacco smoking was correlated with a higher risk of malignancy in the retromolar area and floor of the mouth, while more SCC in the floor of mouth and tongue was observed in patients with habit of drinking [
22]. Yet in our study, we failed to observe a similar result.
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