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01.01.2016 | Original Article

Carious deciduous teeth are a potential source for dental pulp stem cells

verfasst von: Stefanie Bressan Werle, Daniele Lindemann, Daniela Steffens, Flávio Fernando Demarco, Fernando Borba de Araujo, Patrícia Pranke, Luciano Casagrande

Erschienen in: Clinical Oral Investigations | Ausgabe 1/2016

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Abstract

Objective

The objectives of this study are to isolate, cultivate, and characterize stem cells from the pulp of carious deciduous teeth (SCCD) and compare them to those retrieved from sound deciduous teeth (SHED—stem cells from human exfoliated deciduous teeth).

Material and methods

Cells were obtained of dental pulp collected from sound (n = 10) and carious (n = 10) deciduous human teeth. Rate of isolation, proliferation assay (0, 1, 3, 5, and 7 days), STRO-1, mesenchymal (CD29, CD73, and CD90) and hematopoietic surface marker expression (CD14, CD34, CD45, HLA-DR), and differentiation capacity were evaluated.

Results

Isolation success rates were 70 and 80 % from the carious and sound groups, respectively. SCCD and SHED presented similar proliferation rate. There were no statistical differences between the groups for the tested surface markers. The cells from sound and carious deciduous teeth were positive for CD29, CD73, and CD90 and negative for CD14, CD34, CD45, and HLA-DR and were capable of differentiating into osteogenic, chondrogenic, and adipogenic lineages.

Conclusion

SCCD demonstrated a similar pattern of proliferation, immunophenotypical characteristics, and differentiation ability as those obtained from sound deciduous teeth. These SCCD represent a feasible source of stem cells.

Clinical relevance

Decayed deciduous teeth have been usually discarded once the pulp tissue could be damaged and the activity of stem cells compromised. These findings show that stem cells from carious deciduous teeth can be applicable source for cell-based therapies in tissue regeneration.

Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000) Post-natal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 97:13625–13630. doi:10.1073/pnas.240309797 PubMedPubMedCentralCrossRef

Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG et al (2003) SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A 100:5807–5812. doi:10.1073/pnas.0937635100 PubMedPubMedCentralCrossRef

Wang X, Sha XJ, Li GH, Yang FS, Ji K, Wen LY et al (2012) Comparative characterization of stem cells from human exfoliated deciduous teeth and dental pulp stem cells. Arch Oral Biol 57:1231–1240. doi:10.1016/j.archoralbio.2012.02.014 PubMedCrossRef

Arora V, Arora P, Munshi AK (2009) Banking stem cells from human exfoliated deciduous teeth (SHED): saving for the future. J Clin Pediatr Dent 33:289–294PubMedCrossRef

Rosa V, Botero TM, Nör JE (2011) Regenerative endodontics in light of the stem cell paradigm. Int Dent J 61:23–28. doi:10.1111/j.1875-595X.2011.00026.x PubMedPubMedCentralCrossRef

Bernardi L, Luisi SB, Fernandes R, Dalberto TP, Valentim L, Bogo Chies JA et al (2011) The isolation of stem cells from human deciduous teeth pulp is related to the physiological process of resorption. J Endod 37:973–979. doi:10.1016/j.joen.2011.04.010 PubMedCrossRef

Yang R, Chen M, Lee CH, Yoon R, Lal S, Mao JJ (2010) Clones of ectopic stem cells in the regeneration of muscle defects in vivo. PLoS One 5:e13547. doi:10.1371/journal.pone.0013547 PubMedPubMedCentralCrossRef

Gomes JA, Geraldes Monteiro B, Melo GB, Smith RL, Cavenaghi Pereira da Silva M, Lizier NF et al (2010) Corneal reconstruction with tissue-engineered cell sheets composed of human immature dental pulp stem cells. Invest Ophthalmol Vis Sci 51:1408–1414. doi:10.1167/iovs.09-4029 PubMedCrossRef

Young F, Sloan A, Song B (2013) Dental pulp stem cells and their potential roles in central nervous system regeneration and repair. J Neurosci Res 91:1383–1393. doi:10.1002/jnr.23250 PubMedCrossRef

10.

Rosa V, Zhang Z, Grande RH, Nör JE (2013) Dental pulp tissue engineering in full-length human root canals. J Dent Res 92:970–975. doi:10.1177/0022034513505772 PubMedPubMedCentralCrossRef

11.

Prescott RS, Alsanea R, Fayad MI, Johnson BR, Wenckus CS, Hao J et al (2008) In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod 34:421–426. doi:10.1016/j.joen.2008.02.005 PubMedPubMedCentralCrossRef

12.

Zheng Y, Liu Y, Zhang CM, Zhang HY, Li WH, Shi S et al (2009) Stem cells from deciduous tooth repair mandibular defect in swine. J Dent Res 88:249–254. doi:10.1177/0022034509333804 PubMedPubMedCentralCrossRef

13.

d’Aquino R, De Rosa A, Lanza V, Tirino V, Laino L, Graziano A et al (2009) Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cell Mater 12:75–83

14.

Alongi DJ, Yamaza T, Song Y, Fouad AF, Romberg EE, Shi S et al (2010) Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential. Regen Med 5:617–631. doi:10.2217/rme.10.30 PubMedPubMedCentralCrossRef

15.

Wang Z, Pan J, Wright JT, Bencharit S, Zhang S, Everett ET et al (2010) Putative stem cells in human dental pulp with irreversible pulpitis: an exploratory study. J Endod 36:820–825. doi:10.1016/j.joen.2010.02.003 PubMedPubMedCentralCrossRef

16.

Ma D, Gao J, Yue J, Yan W, Fang F, Wu B (2012) Changes in proliferation and osteogenic differentiation of stem cells from deep caries in vitro. J Endod 38:796–802. doi:10.1016/j.joen.2012.02.014 PubMedCrossRef

17.

Pereira LO, Rubini MR, Silva JR, Oliveira DM, Silva IC, Poças-Fonseca MJ et al (2012) Comparison of stem cell properties of cells isolated from normal and inflamed dental pulps. Int Endod J 45:1080–1090. doi:10.1111/j.1365-2591.2012.02068.x PubMedCrossRef

18.

Horst OV, Horst JA, Samudrala R, Dale BA (2011) Caries induced cytokine network in the odontoblast layer of human teeth. BMC Immunol 12:9. doi:10.1186/1471-2172-12-9 PubMedPubMedCentralCrossRef

19.

Goldberg M (2011) Pulp healing and regeneration: more questions than answers. Adv Dent Res 23:270–274. doi:10.1177/0022034511405385 PubMedCrossRef

20.

Bagramian RA, Garcia-Godoy F, Volpe AR (2009) The global increase in dental caries. A pending public health crisis. Am J Dent 22:3–8PubMed

21.

Padilha ARS, Júnior HMM, Barbosa J, Pinto HA, Pucca Jr GA (2011) Ministry of Health. Project SB Brasil 2010: National survey of oral health—key results. Brasilia-DF

22.

Yu S, Diao S, Wang J, Ding G, Yang D, Fan Z (2014) Comparative analysis of proliferation and differentiation potentials of stem cells from inflamed pulp of deciduous teeth and stem cells from exfoliated deciduous teeth. Biomed Res Int 2014:930907. doi:10.1155/2014/930907 PubMedPubMedCentral

23.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317PubMedCrossRef

24.

Kawashima N (2012) Characterisation of dental pulp stem cells: a new horizon for tissue regeneration? Arch Oral Biol 57:1439–1458. doi:10.1016/j.archoralbio.2012.08.010 PubMedCrossRef

25.

Cordeiro MM, Dong Z, Kaneko T, Zhang Z, Miyazawa M, Shi S et al (2008) Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. J Endod 34:962–969. doi:10.1016/j.joen.2008.04.009 PubMedCrossRef

26.

Sakai VT, Zhang Z, Dong Z, Neiva KG, Machado MA, Shi S et al (2010) SHED differentiate into functional odontoblasts and endothelium. J Dent Res 89:791–796. doi:10.1177/0022034510368647 PubMedCrossRef

27.

Demarco FF, Conde MC, Cavalcanti BN, Casagrande L, Sakai VT, Nör JE (2011) Dental pulp tissue engineering. Braz Dent J 22:3–13PubMedPubMedCentralCrossRef

Titel: Carious deciduous teeth are a potential source for dental pulp stem cells
verfasst von: Stefanie Bressan Werle
Daniele Lindemann
Daniela Steffens
Flávio Fernando Demarco
Fernando Borba de Araujo
Patrícia Pranke
Luciano Casagrande
Publikationsdatum: 01.01.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 1/2016
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1477-5

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Carious deciduous teeth are a potential source for dental pulp stem cells