The online version of this article (doi:10.1186/1471-2172-15-4) contains supplementary material, which is available to authorized users.
The author declares that they have no competing interests.
AS performed flow cytometry analysis, analyzed data, wrote the paper. EK collected and analyzed clinical data. EB performed flow cytometry analysis, analyzed data, made graphs. JS collected and analyzed clinical data. OV designed study, analyzed data. VL completed statistical analysis. IS designed study, analyzed data, wrote the paper. All authors read and approved the final manuscript.
Monocytes represent a heterogeneous population of cells subdivided according to the expression level of membrane antigens. A pro-inflammatory (intermediate/nonclassical) subpopulation of monocytes is defined by expression of CD16. CD163 seems to be characteristically preferentially expressed by immunosuppressive monocytes. The aim of our study was to evaluate the distribution of monocyte subpopulations in 71 patients with kidney allograft transplantation.
The phenotype was evaluated by flow cytometry in defined time points. The proportions of peripheral CD14+CD16+ monocytes were downregulated immediately after the kidney transplantation and basiliximab treatment partially attenuated this trend. The transient downregulation of the CD14+CD16+ subpopulation was adjusted to basal values in two months. The proportions of CD14+CD163+ monocytes were transiently upregulated early after the kidney transplantation and remained higher during the first month in most patients. In ATG treated patients, the expansion of CD14+CD163+ monocytes was delayed but their upregulation lasted longer. In vitro data showed the direct effect of ATG and methylprednisolone on expression of CD16 and CD163 molecules while basiliximab did not affect the phenotype of cultured monocytes.
We assume from our data that kidney allograft transplantation is associated with modulation of monocyte subpopulations (CD14+CD16+ and CD14+CD163+) partially affected by an immunosuppressive regime used.
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- CD14+CD16+ and CD14+CD163+ monocyte subpopulations in kidney allograft transplantation
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