Background
Results
Genotyping of Arcobacter isolates
Abundance of putative virulence genes
Cytotoxic effects in vitro
Discussion
Genotyping
Virulence genes
Cytotoxic effects
Conclusions
Methods
Bacterial strains and culture conditions
Genotyping by ERIC-PCR
Primer | Sequence (5′-3′) | Product size (bp) | References |
---|---|---|---|
Genotyping | |||
ERIC 1R | ATG TAA GCT CCT GGG GAT TCA C | Houf et al. [21] | |
ERIC 2 | AAG TAA GTG ACT GGG GTG AGC G | Houf et al. [21] | |
Detection of putative virulence genes | |||
cadF-F | TTA CTC CTA CAC CGT AGT | 283 | Douidah et al. [25] |
cadF-R | AAA CTA TGC TAA CGC TGG TT | Douidah et al. [25] | |
irgA-F | TGC AGA GGA TGC TTG GAG CGT AAC T | 437 | Whiteduck-Leveillee et al. [40] |
irgA-R | GTA TAA CCC CAT TGA TGA GGA GCA | Whiteduck-Leveillee et al. [40] | |
hecA-F | GTG GAA GTA CAA CGA TAG CAG GCT C | 537 | Whiteduck-Leveillee et al. [40] |
hecA-R | GTC TGT TTT AGT TGC TCT GCA GTC | Whiteduck-Leveillee et al. [40] | |
hecB-F | CTA AAC TCT ACA AAT CGT GC | 528 | Whiteduck-Leveillee et al. [40] |
hecB-R | CTT TTG AGT GTT GAC CTC | Whiteduck-Leveillee et al. [40] | |
pldA-F | TTG ACG AGA CAA TAA GTG CAG C | 293 | Whiteduck-Leveillee et al. [40] |
pldA-R | CGT CTT TAT CTT TGC TTT CAG GGA | Whiteduck-Leveillee et al. [40] | |
ciaB-F | TGG GCA GAT GTG GAT AGA GCT TGG A | 284 | Whiteduck-Leveillee et al. [40] |
ciaB-R | TAG TGC TGG TCG TCC CAC ATA AAG | Whiteduck-Leveillee et al. [40] | |
cj1349-F | CCA GAA ATC ACT GGC TTT TGA G | 659 | Whiteduck-Leveillee et al. [40] |
cj1349-R | GGG CAT AAG TTA GAT GAG GTT CC | Whiteduck-Leveillee et al. [40] | |
tlyA-F | CAA AGT CGA AAC AAA GCG ACT G | 230 | Whiteduck-Leveillee et al. [40] |
tlyA-R | TCC ACC AGT GCT ACT TCC TAT A | Whiteduck-Leveillee et al. [40] | |
mviN-F | TGC ACT TGT TGC AAA ACG GTG | 294 | Whiteduck-Leveillee et al. [40] |
mviN-R | TGC TGA TGG AGC TTT TAC GCA AGC | Whiteduck-Leveillee et al. [40] | |
iroE-F | AAT GGC TAT GAT GTT GTT TAC | 415 | Karadas et al. [11] |
iroE-R | TTG CTG CTA TGA AGT TTT | Karadas et al. [11] | |
Detection of putative virulence genes with A. lanthieri specific primers | |||
AL_cdtB F | GCA AAA GGT GAT TGG GCT CC | 303 | Zambri et al. [16] |
AL_cdtB R | TCC TCC AGC TCC TTG AAC AC | Zambri et al. [16] | |
AL_cadF F | TCC AAC TCC AGT TGC TGC TC | 243 | Zambri et al. [16] |
AL_cadF R | TGT CCT TCG ATG TCA GCT TTC | Zambri et al. [16] | |
AL_irgA F | AGA GCT GTT GGT TGG GAT GG | 186 | Zambri et al. [16] |
AL_irgA R | TGC ATT TGC TCT TGT AGG GT | Zambri et al. [16] | |
AL_cdtC F | GAT GAA TCC ACC AGA AAT AGA G | 196 | Zambri et al. [16] |
AL_cdtC R | TTT GGG ATC AAG AGT ATA AAG TTC | Zambri et al. [16] | |
AL_pldA F | TGC TCC ATT TAG AGA AAC TAA C | 132 | Zambri et al. [16] |
AL_pldA R | GAA CGA GAT TCT TCA CCA TCT T | Zambri et al. [16] | |
AL_cdtA F | CAG GAA TAG ATC TCG CTA CAA ATG | 220 | Zambri et al. [16] |
AL_cdtA R | TTT GGT AGA AGA GGA AGT TCA TTG | Zambri et al. [16] | |
AL_mviN F | ACC TTT GGT TCT TCA ACT TTA C | 170 | Zambri et al. [16] |
AL_mviN R | CGT GCT ACC ATA GGA AAT AGG | Zambri et al. [16] | |
AL_ciaB F | GAT AGA TGC TAT TCT GCT CTT G | 207 | Zambri et al. [16] |
AL_ciaB R | ATC TTC ACT AAA TGC TAC TAT T | Zambri et al. [16] | |
AL_tlyA F | GAC ATT GTA ACA TGT GAT GTA TCT T | 125 | Zambri et al. [16] |
AL_tlyA R | TTT ACA TTT GTT CCC ACT TCA AA | Zambri et al. [16] |