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Archives of Virology

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27.03.2017 | Brief Report

Characterization of siRNAs derived from cucumber mosaic virus in infected tobacco plants

verfasst von: Yanhong Qiu, Yongjiang Zhang, Fan Hu, Shuifang Zhu

Erschienen in: Archives of Virology | Ausgabe 7/2017

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Abstract

This study characterized the viral small interfering RNAs (vsiRNAs) from Nicotiana tabacum cv. Samsun infected with a cucumber mosaic virus (CMV) 2b-deficient mutant. Most vsiRNAs were 21 -22 nucleotides in length and the 5′-terminal ends were dominated by A and U, respectively. The observed vsiRNAs were heterogeneously distributed throughout the CMV genome; however, most of the vsiRNAs were derived from sense strands, as opposed to antisense strands. These results demonstrate the conserved and specific function of Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins in tobacco. Finally, it was revealed that vsiRNAs target abundant host genes, indicating complex roles for CMV vsiRNAs during the development of symptoms.

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Titel: Characterization of siRNAs derived from cucumber mosaic virus in infected tobacco plants
verfasst von: Yanhong Qiu
Yongjiang Zhang
Fan Hu
Shuifang Zhu
Publikationsdatum: 27.03.2017
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2017
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3335-z

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