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09.06.2016 | Original Article

Characterization of the nuclear import signal of herpes simplex virus 1 UL31

verfasst von: Mingsheng Cai, Daixiong Chen, Zhancheng Zeng, Hang Yang, Si Jiang, Xiaowei Li, Jingying Mai, Tao Peng, Meili Li

Erschienen in: Archives of Virology | Ausgabe 9/2016

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Abstract

The herpes simplex virus 1 (HSV-1) UL31 protein is a multifunctional nucleoprotein that is important for viral infection; however, little is known concerning its subcellular localization signal. Here, by transfection with a series of HSV-1 UL31 deletion mutants fused to enhanced yellow fluorescent protein (EYFP), a bipartite nuclear localization signal (NLS) was identified and mapped to amino acids (aa) 1 to 27 (MYDTDPHRRGSRPGPYHGKERRRSRSS). Additionally, fluorescence results showed that the predicted nuclear export signal (NES) might be nonfunctional, and the functional NES of UL31 might require a specific conformation. Taken together, these results would provide significant information for the study of the biological function of UL31 during HSV-1 infection.

Ali SA, Steinkasserer A (1995) PCR-ligation-PCR mutagenesis: a protocol for creating gene fusions and mutations. Biotechniques 18:746–750PubMed

Blaho JA, Mitchell C, Roizman B (1994) An amino acid sequence shared by the herpes simplex virus 1 alpha regulatory proteins 0, 4, 22, and 27 predicts the nucleotidylylation of the UL21, UL31, UL47, and UL49 gene products. J Biol Chem 269:17401–17410PubMed

Cai MS, Wang S, Xing JJ, Zheng CF (2011) Characterization of the nuclear import and export signals, and subcellular transport mechanism of varicella-zoster virus ORF9. J Gen Virol 92:621–626CrossRefPubMed

Cai MS, Jiang S, Zeng ZC, Li XW, Mo CC, Yang YJ, Chen CK, Xie PP, Bian Y, Wang JL, Huang JL, Chen DX, Peng T, Li ML (2016) Probing the nuclear import signal and nuclear transport molecular determinants of PRV ICP22. Cell Biosci 6:3CrossRefPubMedPubMedCentral

Cai MS, Si J, Li XW, Zeng ZC, Li ML (2016) Characterization of the nuclear import mechanisms of HSV-1 UL31. Biol Chem 397:555–561CrossRefPubMed

Chang YE, Roizman B (1993) The product of the UL31 gene of herpes simplex virus 1 is a nuclear phosphoprotein which partitions with the nuclear matrix. J Virol 67:6348–6356PubMedPubMedCentral

Chang YE, Van Sant C, Krug PW, Sears AE, Roizman B (1997) The null mutant of the U(L)31 gene of herpes simplex virus 1: construction and phenotype in infected cells. J Virol 71:8307–8315PubMedPubMedCentral

Emmott E, Hiscox JA (2009) Nucleolar targeting: the hub of the matter. EMBO Rep 10:231–238CrossRefPubMedPubMedCentral

Funk C, Ott M, Raschbichler V, Nagel CH, Binz A, Sodeik B, Bauerfeind R, Bailer SM (2015) The herpes simplex virus protein pUL31 escorts nucleocapsids to sites of nuclear egress, a process coordinated by its N-terminal domain. PLoS Pathog 11:e1004957CrossRefPubMedPubMedCentral

10.

Gorlich D, Mattaj IW (1996) Nucleocytoplasmic transport. Science 271:1513–1518CrossRefPubMed

11.

Klupp BG, Granzow H, Fuchs W, Keil GM, Finke S, Mettenleiter TC (2007) Vesicle formation from the nuclear membrane is induced by coexpression of two conserved herpesvirus proteins. Proc Natl Acad Sci USA 104:7241–7246CrossRefPubMedPubMedCentral

12.

Li ML, Cui W, Mo CC, Wang JL, Zhao ZY, Cai MS (2014) Cloning, expression, purification, antiserum preparation and its characteristics of the truncated UL6 protein of herpes simplex virus 1. Mol Biol Rep 41:5997–6002CrossRefPubMed

13.

Li ML, Jiang S, Mo CC, Zeng ZC, Li XW, Chen CK, Yang YJ, Wang JL, Huang JL, Chen DX, Peng T, Cai MS (2015) Identification of molecular determinants for the nuclear import of pseudorabies virus UL31. Arch Biochem Biophys 587:12–17CrossRefPubMed

14.

Li ML, Jiang S, Wang JL, Mo CC, Zeng ZC, Yang YJ, Chen CK, Li XW, Cui W, Huang JL, Peng T, Cai MS (2015) Characterization of the nuclear import and export signals of pseudorabies virus UL31. Arch Virol 160:2591–2594CrossRefPubMed

15.

Mou F, Wills E, Baines JD (2009) Phosphorylation of the U(L)31 protein of herpes simplex virus 1 by the U(S)3-encoded kinase regulates localization of the nuclear envelopment complex and egress of nucleocapsids. J Virol 83:5181–5191CrossRefPubMedPubMedCentral

16.

Nakielny S, Dreyfuss G (1999) Transport of proteins and RNAs in and out of the nucleus. Cell 99:677–690CrossRefPubMed

17.

Park R, Baines JD (2006) Herpes simplex virus type 1 infection induces activation and recruitment of protein kinase C to the nuclear membrane and increased phosphorylation of lamin B. J Virol 80:494–504CrossRefPubMedPubMedCentral

18.

Passvogel L, Klupp BG, Granzow H, Fuchs W, Mettenleiter TC (2015) Functional characterization of nuclear trafficking signals in pseudorabies virus pUL31. J Virol 89:2002–2012CrossRefPubMed

19.

Reynolds AE, Wills EG, Roller RJ, Ryckman BJ, Baines JD (2002) Ultrastructural localization of the herpes simplex virus type 1 UL31, UL34, and US3 proteins suggests specific roles in primary envelopment and egress of nucleocapsids. J Virol 76:8939–8952CrossRefPubMedPubMedCentral

20.

Reynolds AE, Liang L, Baines JD (2004) Conformational changes in the nuclear lamina induced by herpes simplex virus type 1 require genes U(L)31 and U(L)34. J Virol 78:5564–5575CrossRefPubMedPubMedCentral

21.

Roberts KL, Baines JD (2011) UL31 of herpes simplex virus 1 is necessary for optimal NF-kappaB activation and expression of viral gene products. J Virol 85:4947–4953CrossRefPubMedPubMedCentral

22.

Roller RJ, Bjerke SL, Haugo AC, Hanson S (2010) Analysis of a charge cluster mutation of herpes simplex virus type 1 UL34 and its extragenic suppressor suggests a novel interaction between pUL34 and pUL31 that is necessary for membrane curvature around capsids. J Virol 84:3921–3934CrossRefPubMedPubMedCentral

23.

Simpson-Holley M, Baines J, Roller R, Knipe DM (2004) Herpes simplex virus 1 U(L)31 and U(L)34 gene products promote the late maturation of viral replication compartments to the nuclear periphery. J Virol 78:5591–5600CrossRefPubMedPubMedCentral

24.

Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y (2003) Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol 77:1382–1391CrossRefPubMed

25.

Wang KZ, Ni LW, Wang S, Zheng CF (2014) Herpes simplex virus 1 protein kinase US3 hyperphosphorylates p65/RelA and dampens NF-kappaB activation. J Virol 88:7941–7951CrossRefPubMedPubMedCentral

26.

Ye GJ, Roizman B (2000) The essential protein encoded by the UL31 gene of herpes simplex virus 1 depends for its stability on the presence of UL34 protein. Proc Natl Acad Sci USA 97:11002–11007CrossRefPubMedPubMedCentral

Titel: Characterization of the nuclear import signal of herpes simplex virus 1 UL31
verfasst von: Mingsheng Cai
Daixiong Chen
Zhancheng Zeng
Hang Yang
Si Jiang
Xiaowei Li
Jingying Mai
Tao Peng
Meili Li
Publikationsdatum: 09.06.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 9/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-2910-z

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Characterization of the nuclear import signal of herpes simplex virus 1 UL31

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