Introduction
Materials and methods
Subjects
Characteristics | Patients (n = 220) | Controls (n = 200) | t/χ2 | P value |
---|---|---|---|---|
Gender, n (%) | 0.016 | 0.898 | ||
Male | 124 (56.6) | 112 (56.0) | ||
Female | 96 (43.4) | 88 (44.0) | ||
Age (years) | 61.1 ± 11.4 | 60.8 ± 11.8 | 0.234 | 0.815 |
Site of plaques | ||||
Carotid artery | 6 (2.7) | – | ||
Carotid bifurcation | 52 (23.7) | – | ||
Internal carotid artery | 39 (17.8) | – | ||
Internal carotid bifurcation | 2 (0.9) | – | ||
Common carotid artery | 100 (45.7) | – | ||
Subclavian artery | 20 (9.1) | – |
Antibody testing
Antigens | Sequence (N → C) | NCBI accession |
---|---|---|
IL1α | H-WETHGTKNYFTSVAHPNLFIATKQDYWVC-OH | NP_000566 |
IL1β-1 | H-SLNCTLRDSQQKSLVMSGPYELKALHLQG-OH | NP_000567 |
IL1β-2 | H-KHAYYSGNEDDLFFEADGPKQMKCH-OH | NP_000567 |
IL6 | H-LTKLQAQNQWLQDMTTHLILRSC-OH | NP_000591 |
IL8 | H-DCQCIKTYSKPFHPKFIKELRVIESD-OH | NP_000575 |
TNFα-1 | H-CQLQWLNRRANALLANGVELRDNQLV-OH | NP_000585 |
TNFα-2 | H-KSAIKSPCQRETPEGAEAKPWYEPK-OH | NP_000585 |
Data analysis
Results
Factors | Number of plates | Mean ± SD | CV (%) |
---|---|---|---|
IL1β-1 | 20 | 0.67 ± 0.11 | 16.94% |
IL6 | 20 | 1.21 ± 0.15 | 12.11% |
IL8 | 20 | 1.28 ± 0.12 | 9.42% |
TNFα-1 | 20 | 1.24 ± 0.07 | 5.87% |
IL1α | 20 | 0.93 ± 0.07 | 7.45% |
IL1β-2 | 20 | 1.14 ± 0.10 | 9.12% |
TNFα-2 | 20 | 1.79 ± 0.29 | 16.10% |
Factors | Atherosclerosis (Mean ± SD) | Control (Mean ± SD) |
t
| P value |
---|---|---|---|---|
IL1β-1 | ||||
Male | 0.651 ± 0.310 (124) | 0.657 ± 0.358 (112) | −0.138 | 0.890 |
Female | 0.674 ± 0.331 (96) | 0.634 ± 0.419 (88) | 0.709 | 0.480 |
Total | 0.661 ± 0.319 (220) | 0.647 ± 0.385 (200) | 0.399 | 0.690 |
IL6 | ||||
Male | 1.204 ± 0.357 (124) | 1.188 ± 0.350 (112) | 0.353 | 0.725 |
Female | 1.297 ± 0.437 (96) | 1.199 ± 0.545 (88) | 1.349 | 0.179 |
Total | 1.244 ± 0.396 (220) | 1.193 ± 0.445 (200) | 1.259 | 0.209 |
IL8 | ||||
Male | 1.087 ± 0.300 (124) | 1.092 ± 0.295 (112) | −0.105 | 0.916 |
Female | 1.145 ± 0.292 (96) | 1.101 ± 0.307 (88) | 1.007 | 0.315 |
Total | 1.113 ± 0.298 (220) | 1.096 ± 0.300 (200) | 0.581 | 0.561 |
TNFα-1 | ||||
Male | 1.198 ± 0.296 (124) | 1.145 ± 0.265 (112) | 1.438 | 0.152 |
Female | 1.291 ± 0.302 (96) | 1.133 ± 0.253 (88) | 3.810 |
<0.001
|
Total | 1.238 ± 0.301 (220) | 1.140 ± 0.259 (200) | 3.588 |
<0.001
|
IL1α | ||||
Male | 0.883 ± 0.162 (124) | 0.852 ± 0.159 (112) | 1.490 | 0.138 |
Female | 0.906 ± 0.148 (96) | 0.835 ± 0.176 (88) | 2.964 |
0.003
|
Total | 0.893 ± 0.156 (220) | 0.845 ± 0.166 (200) | 3.084 |
0.002
|
IL1β-2 | ||||
Male | 1.008 ± 0.201 (124) | 1.008 ± 0.233 (112) | 0.031 | 0.975 |
Female | 1.085 ± 0.270 (96) | 1.002 ± 0.224 (88) | 2.248 |
0.026
|
Total | 1.042 ± 0.236 (220) | 1.005 ± 0.229 (200) | 1.603 | 0.110 |
TNFα-2 | ||||
Male | 1.385 ± 0.427 (124) | 1.457 ± 0.497 (112) | −1.195 | 0.233 |
Female | 1.486 ± 0.426 (96) | 1.492 ± 0.431 (88) | −0.090 | 0.928 |
Total | 1.429 ± 0.428 (220) | 1.472 ± 0.468 (200) | −0.991 | 0.322 |
Factors | Adjusted R2 | P value* | β & 95% CI |
---|---|---|---|
IL1β-1 | |||
Male | −0.001 | 0.866 | −0.007 (− 0.093, 0.078) |
Female | −0.008 | 0.481 | 0.039 (−0.071, 0.150) |
Total | −0.004 | 0.698 | 0.013 (−0.054, 0.081) |
IL6 | |||
Male | −0.006 | 0.736 | 0.016 (−0.075, 0.107) |
Female | 0.003 | 0.179 | 0.098 (−0.045, 0.242) |
Total | 0.001 | 0.204 | 0.052 (−0.029, 0.133) |
IL8 | |||
Male | −0.009 | 0.915 | −0.004 (− 0.081, 0.073) |
Female | 0.003 | 0.316 | 0.045 (− 0.043, 0.132) |
Total | −0.001 | 0.564 | 0.017 (−0.041, 0.074) |
TNFα-1 | |||
Male | 0.012 | 0.161 | 0.051 (−0.021, 0.124) |
Female | 0.073 |
< 0.001
| 0.157 (0.076, 0.239) |
Total | 0.038 |
< 0.001
| 0.098 (0.044, 0.152) |
IL1α | |||
Male | 0.012 | 0.146 | 0.030 (−0.011, 0.071) |
Female | 0.044 |
0.003
| 0.071 (0.024, 0.118) |
Total | 0.025 |
0.002
| 0.048 (0.017, 0.079) |
IL1β-2 | |||
Male | 0.009 | 0.988 | −0.0004 (−0.056, 0.055) |
Female | 0.023 |
0.026
| 0.083 (0.010, 0.155) |
Total | 0.017 | 0.111 | 0.036 (−0.008, 0.080) |
TNFα-2 | |||
Male | 0.012 | 0.215 | −0.074 (− 0.192, 0.044) |
Female | −0.011 | 0.928 | −0.006 (− 0.131, 0.120) |
Total | 0.006 | 0.316 | −0.044 (− 0.130, 0.042) |
Factors | df | Coefficients of correlation | P value |
---|---|---|---|
IL1β-1 | 218 | 0.053 | 0.432 |
IL6 | 218 | 0.007 | 0.920 |
IL8 | 218 | 0.060 | 0.379 |
TNFα-1 | 218 | 0.003 | 0.968 |
IL1α | 218 | 0.058 | 0.397 |
IL1β-2 | 218 | −0.081 | 0.234 |
TNFα-2 | 218 | −0.064 | 0.347 |