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01.04.2016 | Original Article

CO₂ laser irradiation enhances CaF₂ formation and inhibits lesion progression on demineralized dental enamel—in vitro study

verfasst von: Bruna R. Zancopé, Lívia P. Rodrigues, Thais M. Parisotto, Carolina Steiner-Oliveira, Lidiany K. A. Rodrigues, Marinês Nobre-dos-Santos

Erschienen in: Lasers in Medical Science | Ausgabe 3/2016

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Abstract

This study evaluated if Carbon dioxide (CO₂) (λ 10.6 μm) laser irradiation combined with acidulated phosphate fluoride gel application (APF gel) enhances “CaF₂” uptake by demineralized enamel specimens (DES) and inhibits enamel lesion progression. Thus, two studies were conducted and DES were subjected to APF gel combined or not with CO₂ laser irradiation (11.3 or 20.0 J/cm², 0.4 or 0.7 W) performed before, during, or after APF gel application. In study 1, 165 DES were allocated to 11 groups. Fluoride as “CaF₂ like material” formed on enamel was determined in 100 DES (n = 10/group), and the surface morphologies of 50 specimens were evaluated by scanning electron microscopy (SEM) before and after “CaF₂” extraction. In study 2, 165 DES (11 groups, n = 15), subjected to the same treatments as in study 1, were further subjected to a pH-cycling model to simulate a high cariogenic challenge. The progression of demineralization in DES was evaluated by cross-sectional microhardness and polarized light microscopy analyses. Laser at 11.3 J/cm² applied during APF gel application increased “CaF₂” uptake on enamel surface. Laser irradiation and APF gel alone arrested the lesion progression compared with the control (p < 0.05). Areas of melting, fusion, and cracks were observed. CO₂ laser irradiation, combined with a single APF application enhanced “CaF₂” uptake on enamel surface and a synergistic effect was found. However, regarding the inhibition of caries lesion progression, no synergistic effect could be demonstrated. In conclusion, the results have shown that irradiation with specific laser parameters significantly enhanced CaF₂ uptake by demineralized enamel and inhibited lesion progression.

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Titel: CO2 laser irradiation enhances CaF2 formation and inhibits lesion progression on demineralized dental enamel—in vitro study
verfasst von: Bruna R. Zancopé
Lívia P. Rodrigues
Thais M. Parisotto
Carolina Steiner-Oliveira
Lidiany K. A. Rodrigues
Marinês Nobre-dos-Santos
Publikationsdatum: 01.04.2016
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 3/2016
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-016-1900-4

Springer Medizin

Abstract

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