Introduction
Materials and methods
Materials
Isolation, culture and identification of BMSC
Grouping
Macro observation
Wet/dry weight ratio of lung tissue
Determination of cytokine levels
Nucleoprotein extraction and Measurement of NF-кBp65 expression
Statistical analysis
Results
In vitroculture and identification of BMSC
General conditions and survival of rats
Wet/dry weight ratio of lung tissue
Groups | 1d | 3d | 7d | 14d |
---|---|---|---|---|
PQ | 4.51 ± 0.18a | 5.52 ± 0.21a | 7.62 ± 0.26a | |
BMSC | 4.51 ± 0.34
b
| 5.49 ± 0.42
b
| 6.15 ± 0.42c | 5.65 ±0.57 |
MP | 4.42 ± 0.29
d
| 5.32 ± 0.37
d
| 5.94 ± 0.28
d
| |
BMSC + MP | 4.40 ± 0.51
ef
| 5.18 ± 0.19
eg
| 4.79 ± 0.31
eg
| 4.71 ±0.25
e
|
Normal control | 4.32 ± 0.31 |
Plasma levels of TNF-α, IL-1β, IL-6 and IL-10
Group | Post-poisoning day | TNF-α | IL-1β | IL-6 | IL-10 |
---|---|---|---|---|---|
PQ | 1 | 83.13 ± 10.29 a | 30.25 ± 4.68 a | 35.47 ± 2.31 a | 18.62 ± 5.46 a |
3 | 121.51 ± 10.85 a | 48.90 ± 4.62 a | 98.15 ± 9.44 a | 47.86 ± 7.51 a | |
7 | 245.63 ± 12.11 a | 102.63 ± 8.71 a | 145.61 ± 15.38 a | 66.24 ± 5.83 a | |
14 | |||||
BMSC | 1 | 79.66 ± 8.31 c | 21.05 ± 6.47 c | 31.37 ± 5.42
d
| 18.17 ± 4.63
b
|
3 | 105.35 ± 8.32 c | 43.11 ± 5.15 c | 82.40 ± 7.16 c | 59.36 ± 7.27 c | |
7 | 144.84 ± 10.73 c | 68.78 ± 4.32 c | 121.27 ± 9.54 c | 83.42 ± 8.03 c | |
14 | 65.20 ± 6.64 | 21.89 ± 3.56 a | 84.53 ± 9.07 a | 108.05 ± 10.16 a | |
MP | 1 | 51.17 ± 7.33
e
| 15.62 ± 4.81
e
| 19.28 ± 8.66
e
| 29.75 ± 8.09
e
|
3 | 89.15 ±8.65
e
| 31.72 ±6.18
e
| 70.50 ±8.13
e
| 65.39 ±5.24 | |
7 | 125.63± 10.44
e
| 50.64 ±5.74
e
| 118.69 ±10.37 | 85.17 ±7.36 | |
14 | |||||
BMSC + MP | 1 | 50.43 ± 9.61
ef
| 13.53 ± 4.17
ef
| 17.11 ± 5.36
ef
| 27.91 ± 6.39
ef
|
3 | 67.47 ±9.25
e
| 29.56 ±4.52
ef
| 71.73 ±8.25
ef
| 70.69 ±10.42
e
| |
7 | 71.84 ±8.11
eg
| 35.18 ±4.83
eg
| 103.68 ±7.70
eg
| 92.38 ±6.17
eg
| |
14 | 49.11 ±7.62
e
| 14.56 ±2.95
e
| 49.27 ±10.34
e
| 117.41± 8.82
e
| |
Normal control | 32.08 ± 3.13 | 10.16 ± 2.56 | 8.70 ± 0.93 | 10.04 ± 1.96 |
Plasma levels of MDA and SOD
Group | MDA (nmol/ml) | SOD (U/ml) | ||||||
---|---|---|---|---|---|---|---|---|
1d | 3d | 7d | 14d | 1d | 3d | 7d | 14d | |
PQ | 4.03 ±0.61 a | 4.65 ±0.78 a | 5.07 ±0.64 a | 287.66 ±11.53 a | 246.32 ±16.31 a | 212.58 ±19.22 a | ||
BMSC | 3.97 ±0.70
b
| 4.32 ±0.48 c | 4.16 ±0.81 c | 3.63±0.56 | 285.17 ±13.69
b
| 261.24 ±20.74 c | 269.74 ±15.64 c | 284.42± 17.56 |
MP | 3.82 ±0.58
d
| 4.49 ±0.46 | 4.06 ±0.62 | 294.52 ±10.14
d
| 265.43 ±15.26 | 280.15 ±14.58 | ||
BMSC + MP | 3.78 ±0.74 | 4.20 ±0.29
de
| 3.79 ±0.51
de
| 3.31±0.68 | 292.38 ±9.06 | 278.66 ±11.84
de
| 302.37 ±10.11
de
| 304.15± 10.63 |
Normal control | 3.06 ± 0.24 | 308.29 ± 18.83 |
Expression of NF-кB p65 in lung tissue
Group | 1d | 3d | 7d | 14d |
---|---|---|---|---|
PQ | 139.54 ± 12.46a | 188.78 ± 17.73a | 140.49 ± 9.21a | |
BMSC | 125.32 ± 10.63
b
| 146.56 ± 12.55
b
| 101.84 ± 7.45
b
| 95.77 ± 8.12 |
MP | 116.47 ± 8.91 c | 127.82 ± 10.39c | 103.37 ± 11.44
e
| |
BMSC + MP | 115.15 ± 10.48cf | 125.16 ± 9.83cf | 96.41 ± 12.32d | 89.75 ± 7.60d |
Normal control | 82.36 ± 6.53 |