Background
Methods
Patients
Deoxyribonucleic Acid Extraction
Genotyping
Gene | SNP | Sense primer | Antisense primer | Probe/restriction enzyme |
---|---|---|---|---|
TNF-α | G- 308 A | 5'-TAGGTTTTGAGGGGCATGGGGAC-3' | 5'- TCTCGGTTTCTTCTCCATCG -3' | 5'-TAGGTTTTGAGGGGCATGGGGAC-3'-fluorescein 5'-LC Red640-GGGTTCAGCCTCCAGGGTCCTACACAC-3'-phosphate |
Il-1β | C3953T | 5'-CACTCCCAGCTTCATCCCTA-3' | 5'-AGGTGCATCGTGCACATAAG-3' |
Taq I |
PAI-1 | 4G/5G | 5G allele; 5 ' -GTCTGGACACGTGGGGG-3 ' 4G allele; 5 ' -GTCTGGACACGTGGGGA-3 ' | 5 ' -TGCAGCCAGCCACGTGATTGTCTAG-3 ' | None |
uPA | C 422 T | 5'-biotin-ACTGCAGGAACCCAGACAAC-3' | 5'-AGGGAGGCAGGTAGGAGAAA-3' | 5'-GCCTAAAGCCGCTTGTCCAA-3' 5'-GGCCTAAAGCTGCTTGTCCAA-3' |
TLR4 | A 896G | 5'-biotin-AGTCCATCGTTTGGTTCTGG-3' | 5'-AATAGTCACACTCACCAGGGAAA-3' | 5'-AGTCAATAATATCATCGAGGTAG-3' 5'-AGTCAATAATACCATCGAGGTAG-3' |
CD14 | C-159T | 5'-biotin-CACCCACCAGAGAAGGCTTA-3' | 5'-ATCACCTCCCCACCTCTCTT-3' | 5'-GGAGGGGGGCCGTAACA-3' 5'-GGGAGGGGGACCGTAACAG-3' |
Hardy-Weinberg equilibrium (HWE)
Statistics
Results
Patient characteristics
Genotypes
Genotype | Sepsis (%) | Severe sepsis (%) | Septic shock (%) | All (%) | P value |
---|---|---|---|---|---|
TNF-α (G-308A) | |||||
GG | 134 (60) | 35 (58) | 13 (65) | 182 (60) | |
GA | 80 (36) | 20 (33) | 6 (30) | 106 (35) | |
AA | 10 (4) | 5 (9) | 1 (5) | 16 (5) | 0.92 |
Il-1β (C3953T) | |||||
CC | 135 (57) | 32 (53) | 11 (52) | 178 (56) | |
CT | 83 (35) | 24 (40) | 10 (48) | 117 (37) | |
TT | 18 (8) | 4 (7) | 0 | 22 (7) | 0.5 |
PAI-1 (4G/5G) | |||||
4G-4G | 67 (28) | 16 (27) | 7 (33) | 90 (28) | |
4G-5G | 134 (58) | 37 (62) | 12 (57) | 183 (58) | |
5G-5G | 35 (14) | 7 (11) | 2 (10) | 44 (14) | 0.89 |
uPA (C422T) | |||||
CC | 135 (58) | 41 (67) | 11 (52) | 187 (60) | |
CT | 87 (38) | 19 (31) | 8 (38) | 114 (36) | |
TT | 9 (4) | 1 (2) | 2 (10) | 12 (4) | 0.30 |
TLR4 (A 896G) | |||||
AA | 204 (91) | 52 (91) | 18 (86) | 274 (91) | |
AG | 19 (9) | 5 (9) | 3 (14) | 27 (9) | |
GG | 0 | 0 | 0 | 0 | 0.52 |
CD14 (C-159T) | |||||
CC | 72 (31) | 13 (22) | 7 (33) | 92 (29) | |
CT | 117 (50) | 30 (51) | 9 (43) | 156 (50) | |
TT | 45 (19) | 16 (27) | 5 (24) | 66 (21) | 0.57 |
Genotypes and baseline characteristics
Disease severity and genotype
Mortality and genotype
Genotype | In-hospital mortality (%) | P value |
---|---|---|
TNF-α (G-308A) | ||
GG | 34/182 (19) | |
GA | 20/106 (19) | |
AA | 6/16 (38) | 0.19 |
Il-1β (C3953T) | ||
CC | 32/178 (18) | |
CT | 25/117 (21) | |
TT | 5/22 (23) | 0.61 |
PAI-1 (4G/5G) | ||
4G-4G | 15/90 (17) | |
4G-5G | 37/183 (20) | |
5G-5G | 11/44 (25) | 0.61 |
uPA (C422T) | ||
CC | 40/187 (21) | |
CT | 20/114 (18) | |
TT | 2/12 (17) | 0.86 |
TLR4 (A 896G) | ||
AA | 49/274 (18) | |
AG | 8/27 (30) | |
GG | - | 0.11 |
CD14 (C-159T) | ||
CC | 18/92 (20) | |
CT | 33/156 (21) | |
TT | 11/66 (17) | 0.90 |
Multivariate analysis of factors associated with outcome
Model 1 | Model 2 | Model 3 | ||||
---|---|---|---|---|---|---|
Variable | RR (95% CI) | P value | RR (95% CI) | P value | RR (95% CI) | P value |
Age, per year increment
| 1.02 (1.00–1.04) | 0.026 | - | 1.02 (1.00–1.03) | 0.053 | |
Diseases severity
| - | - | ||||
Sepsis | 1.0 | |||||
Severe sepsis | 1.25 (0.65–2.39) | |||||
Septic shock | 1.70 (0.48–6.05) | 0.674 | ||||
Intensive care admission
| - | - | ||||
No | 1.0 | |||||
Yes | 2.50 (0.85–7.42) | 0.098 | ||||
Polymicrobial infection
| - | |||||
No | 1.0 | 1.0 | ||||
Yes | 3.02 (1.58–7.41) | 0.001 | 2.52 (1.27–5.01) | 0.008 | ||
Hemoglobin, per μmol/L increase
| - | 0.71 (0.52–0.97) | 0.031 | 0.70 (0.56–0.88) | 0.002 | |
Leukocyte count, per 10
9
/mL increase
| - | 1.00 (0.96–1.05) | 0.795 | - | ||
C-reactive protein, per milligram/L increase
| - | 1.00 (0.99–1.00) | 0.287 | - | ||
Urea, per μmol/L increase
| - | 1.02 (0.98–1.06) | 0.234 | - | ||
Alanine transferase, per unit/L increase
| - | 0.99 (0.98–1.00) | 0.195 | - |