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Clinical Oral Investigations

Erschienen in:

28.06.2018 | Original Article

Comparative differentiation analysis of distinct oral tissue-derived cells in response to osteogenic stimulation

verfasst von: S. Proksch, K. Kirsch, K. Vach, E. Hellwig, P. Tomakidi

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2019

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Abstract

Background

Mixed cell populations from oral tissues may be superior to pure stem cells for regenerative approaches. Therefore, the aim of the present study was to explore the osteogenic potential of mixed cells derived from oral connective tissues compared to alveolar osteoblasts.

Materials and methods

Primary cells were isolated from the alveolar bone, periodontal ligament and gingiva. Following characterization by colony formation, growth capacity and flow cytometry, all cells were subjected to osteogenic differentiation induction and screened for a large panel of osteogenic markers using western blots, qPCR arrays, and matrix mineralization and alkaline phosphatase quantification.

Results

Non-induced mixed cells from gingiva showed higher colony formation efficiency but decreased proliferation compared to non-induced periodontal mixed cells, while both entities revealed similar surface markers tested in this setup. Following osteogenic induction, all cell populations individually expressed receptors with distinctively activated downstream effectors. Gene expression of induced periodontal mixed cells was similar to alveolar osteoblasts, but was differently modulated in gingival mixed cells. The latter failed to achieve osteogenic differentiation in terms of matrix mineralization and alkaline phosphatase activity, which was well observed in periodontal mixed cells and osteoblasts.

Conclusion

Mixed cells from periodontal ligament but not from gingiva feature an inherent osteogenic capacity in vitro. From these results, it can be concluded that periodontal cells do not require further stem cell enrichment in order to qualify for bone regeneration.

Clinical relevance

Our data contribute to the development of novel cell-based therapies using mixed cells from the periodontal ligament in regenerative periodontics.

Nur mit Berechtigung zugänglich

Hu L, Liu Y and Wang S (2017) Stem cell-based tooth and periodontal regeneration. Oral Dis . doi: 10.1111/odi.12703

Egusa H, Sonoyama W, Nishimura M, Atsuta I, Akiyama K (2012) Stem cells in dentistry—part I: stem cell sources. J Prosthodont Res 56:151–165. https://doi.org/10.1016/j.jpor.2012.06.001 CrossRefPubMed

Volponi AA, Sharpe PT (2013) The tooth—a treasure chest of stem cells. Br Dent J 215:353–358. https://doi.org/10.1038/sj.bdj.2013.959 CrossRefPubMed

Hynes K, Menicanin D, Gronthos S, Bartold PM (2012) Clinical utility of stem cells for periodontal regeneration. Periodontol 59:203–227. https://doi.org/10.1111/j.1600-0757.2012.00443.x CrossRef

Han J, Menicanin D, Gronthos S, Bartold PM (2014) Stem cells, tissue engineering and periodontal regeneration. Aust Dent J 59(Suppl 1):117–130. https://doi.org/10.1111/adj.12100 CrossRefPubMed

Tassi SA, Sergio NZ, Misawa MYO, Villar CC (2017) Efficacy of stem cells on periodontal regeneration: systematic review of pre-clinical studies. J Periodontal Res 52:793–812. https://doi.org/10.1111/jre.12455 CrossRefPubMed

Avinash K, Malaippan S, Dooraiswamy JN (2017) Methods of isolation and characterization of stem cells from different regions of oral cavity using markers: a systematic review. Int J Stem Cells 10:12–20. https://doi.org/10.15283/ijsc17010 CrossRefPubMedPubMedCentral

Fawzy El-Sayed KM, Dorfer CE Gingival mesenchymal stem/progenitor cells: a unique tissue engineering gem. Stem Cells Int 2016, 2016:7154327–7154316. https://doi.org/10.1155/2016/7154327

Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S (2004) Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 364:149–155. https://doi.org/10.1016/s0140-6736(04)16627-0 CrossRefPubMed

10.

Shi S, Gronthos S (2003) Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res 18:696–704. https://doi.org/10.1359/jbmr.2003.18.4.696 CrossRefPubMed

11.

Lekic P, Rojas J, Birek C, Tenenbaum H, McCulloch CA (2001) Phenotypic comparison of periodontal ligament cells in vivo and in vitro. J Periodontal Res 36:71–79CrossRefPubMed

12.

Fournier BP, Larjava H, Hakkinen L (2013) Gingiva as a source of stem cells with therapeutic potential. Stem Cells Dev 22:3157–3177. https://doi.org/10.1089/scd.2013.0015 CrossRefPubMed

13.

About I (2018) "The stem cell fashion": do we need only stem cells for tissue regeneration? Clin Oral Invest 22:553–554. https://doi.org/10.1007/s00784-017-2316-7 CrossRef

14.

Mo M, Wang S, Zhou Y, Li H, Wu Y (2016) Mesenchymal stem cell subpopulations: phenotype, property and therapeutic potential. Cell Mol Life Sci 73:3311–3321. https://doi.org/10.1007/s00018-016-2229-7 CrossRefPubMed

15.

Jones DL, Wagers AJ (2008) No place like home: anatomy and function of the stem cell niche. Nat Rev Mol Cell Biol 9:11–21. https://doi.org/10.1038/nrm2319 CrossRefPubMed

16.

Xiao L, Nasu M (2014) From regenerative dentistry to regenerative medicine: progress, challenges, and potential applications of oral stem cells. Stem Cells Cloning 7:89–99. https://doi.org/10.2147/sccaa.s51009 CrossRefPubMedPubMedCentral

17.

Proksch S, Steinberg T, Vach K, Hellwig E, Tomakidi P (2014) Shaping oral cell plasticity to osteogenic differentiation by human mesenchymal stem cell coculture. Cell Tissue Res 356:159–170. https://doi.org/10.1007/s00441-013-1777-5 CrossRefPubMed

18.

Mostafa NZUH, Varkey M, Dederich DN, Doschak MR, El-Bialy TH (2011) In vitro osteogenic induction of human gingival fibroblasts for bone regeneration. Open Dent J 5:139–145CrossRefPubMedPubMedCentral

19.

Zhou Y, Hutmacher DW, Sae-Lim V, Zhou Z, Woodruff M, Lim TM (2008) Osteogenic and adipogenic induction potential of human periodontal cells. J Periodontol 79:525–534. https://doi.org/10.1902/jop.2008.070373 CrossRefPubMed

20.

Ivanovski S, Li H, Haase HR, Bartold PM (2001) Expression of bone associated macromolecules by gingival and periodontal ligament fibroblasts. J Periodontal Res 36:131–141CrossRefPubMed

21.

Otabe K, Muneta T, Kawashima N, Suda H, Tsuji K, Sekiya I (2012) Comparison of gingiva, dental pulp, and periodontal ligament cells from the standpoint of mesenchymal stem cell properties. Cell Med 4:13–21. https://doi.org/10.3727/215517912X653319 CrossRefPubMedPubMedCentral

22.

Kundrotas G (2012) Surface markers distinguishing mesenchymal stem cells from fibroblasts. Acta Med Lituan 19:75–79

23.

Pouly J, Bruneval P, Mandet C, Proksch S, Peyrard S, Amrein C, Bousseaux V, Guillemain R, Deloche A, Fabiani JN, Menasche P (2008) Cardiac stem cells in the real world. J Thorac Cardiovasc Surg 135:673–678. https://doi.org/10.1016/j.jtcvs.2007.10.024 CrossRefPubMed

24.

Pisciotta A, Carnevale G, Meloni S, Riccio M, De Biasi S, Gibellini L, Ferrari A, Bruzzesi G, De Pol A (2015) Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Dev Biol 15:14. https://doi.org/10.1186/s12861-015-0065-x CrossRefPubMedPubMedCentral

25.

Gagari E, Rand MK, Tayari L, Vastardis H, Sharma P, Hauschka PV, Damoulis PD (2006) Expression of stem cell factor and its receptor, c-kit, in human oral mesenchymal cells. Eur J Oral Sci 114:409–415. https://doi.org/10.1111/j.1600-0722.2006.00388.x CrossRefPubMed

26.

Sorrell JM, Caplan AI (2009) Fibroblasts-a diverse population at the center of it all. Int Rev Cell Mol Biol 276:161–214. https://doi.org/10.1016/s1937-6448(09)76004-6 CrossRefPubMed

27.

Bradley JE, Ramirez G, Hagood JS (2009) Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors 35:258–265. https://doi.org/10.1002/biof.41 CrossRefPubMedPubMedCentral

28.

Wang Z, Yan X (2013) CD146, a multi-functional molecule beyond adhesion. Cancer Lett 330:150–162. https://doi.org/10.1016/j.canlet.2012.11.049 CrossRefPubMed

29.

Mangialardi G, Cordaro A, Madeddu P (2016) The bone marrow pericyte: an orchestrator of vascular niche. Regen Med 11:883–895. https://doi.org/10.2217/rme-2016-0121 CrossRefPubMedPubMedCentral

30.

Xu J, Wang W, Kapila Y, Lotz J, Kapila S (2009) Multiple differentiation capacity of STRO-1+/CD146+ PDL mesenchymal progenitor cells. Stem Cells Dev 18:487–496. https://doi.org/10.1089/scd.2008.0113 CrossRefPubMed

31.

Mrozik K, Gronthos S, Shi S, Bartold PM (2017) A method to isolate, purify, and characterize human periodontal ligament stem cells. Methods Mol Biol 1537:413–427. https://doi.org/10.1007/978-1-4939-6685-1_24 CrossRefPubMed

32.

El-Sayed KM, Paris S, Graetz C, Kassem N, Mekhemar M, Ungefroren H, Fandrich F, Dorfer C (2015) Isolation and characterisation of human gingival margin-derived STRO-1/MACS(+) and MACS(−) cell populations. Int J Oral Sci 7:80–88. https://doi.org/10.1038/ijos.2014.41 CrossRefPubMed

33.

Beederman MLJ, Nan G, Wang J, Liu X, Yin L, Li R, Shui W, Zhang H, Kim SH, Zhang W, Zhang J, Kong Y, Denduluri S, Rogers MR, Pratt A, Haydon RC, Luu HH, Angeles J, Shi LL, He TC (2013) BMP signaling in mesenchymal stem cell differentiation and bone formation. J Biomed Sci Eng 6:32–52CrossRefPubMedPubMedCentral

34.

Ishibashi O, Ikegame M, Takizawa F, Yoshizawa T, Moksed MA, Iizawa F, Mera H, Matsuda A, Kawashima H (2010) Endoglin is involved in BMP-2-induced osteogenic differentiation of periodontal ligament cells through a pathway independent of Smad-1/5/8 phosphorylation. J Cell Physiol 222:465–473. https://doi.org/10.1002/jcp.21968 CrossRefPubMed

35.

Torii D, Tsutsui TW, Watanabe N, Konishi K (2016) Bone morphogenetic protein 7 induces cementogenic differentiation of human periodontal ligament-derived mesenchymal stem cells. Odontology 104:1–9. https://doi.org/10.1007/s10266-014-0182-1 CrossRefPubMed

36.

Luan X, Dangaria S, Ito Y, Walker CG, Jin T, Schmidt MK, Galang MT, Druzinsky R (2009) Neural crest lineage segregation: a blueprint for periodontal regeneration. J Dent Res 88:781–791. https://doi.org/10.1177/0022034509340641 CrossRefPubMedPubMedCentral

37.

Matsubara T, Suardita K, Ishii M, Sugiyama M, Igarashi A, Oda R, Nishimura M, Saito M, Nakagawa K, Yamanaka K, Miyazaki K, Shimizu M, Bhawal UK, Tsuji K, Nakamura K, Kato Y (2005) Alveolar bone marrow as a cell source for regenerative medicine: differences between alveolar and iliac bone marrow stromal cells. J Bone Miner Res 20:399–409. https://doi.org/10.1359/jbmr.041117 CrossRefPubMed

38.

Paldi A (2013) Effects of the in vitro manipulation of stem cells: epigenetic mechanisms as mediators of induced metabolic fluctuations. Epigenomics 5:429–437. doi: 10.2217/epi.13.35

39.

Rodier F, Campisi J (2011) Four faces of cellular senescence. J Cell Biol 192:547–556. https://doi.org/10.1083/jcb.201009094 CrossRefPubMedPubMedCentral

40.

Choi JK, Hwang HI, Jang YJ (2015) The efficiency of the in vitro osteo/dentinogenic differentiation of human dental pulp cells, periodontal ligament cells and gingival fibroblasts. Int J Mol Med 35:161–168. https://doi.org/10.3892/ijmm.2014.1986 CrossRefPubMed

Titel: Comparative differentiation analysis of distinct oral tissue-derived cells in response to osteogenic stimulation
verfasst von: S. Proksch
K. Kirsch
K. Vach
E. Hellwig
P. Tomakidi
Publikationsdatum: 28.06.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 3/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2529-4

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Comparative differentiation analysis of distinct oral tissue-derived cells in response to osteogenic stimulation

Abstract

Background

Materials and methods

Results

Conclusion

Clinical relevance

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Klimawandel und Gesundheit: Was Sie in der Hausarztpraxis wissen müssen und tun können

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Abstract

Background

Materials and methods

Results

Conclusion

Clinical relevance

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