This study aims to evaluate the repeatability, reproducibility and agreement of the CASIA SS-1000 and Pentacam in measuring the anterior segment parameters in an eye, including corneal curvature and corneal thickness maps. Of the eight parameters studied, flat and steep meridians of the anterior corneal curvature (AKf and AKs) and CCT/TCT are the most important parameters in making clinical decisions.
Anterior corneal curvatures
Anterior curvatures, along with axial length, are the key factors in calculating IOL power. A difference of more than 1 D in anterior corneal curvature is considered as clinically significant. The repeatability and reproducibility were excellent (SDs < 0.3 D). However, the CASIA SS-1000 had a better reproducibility in measuring flat axis (ratio = 0.58). The average difference in AKf and AKs between the two devices was 0.01 D and − 0.02 D, respectively. LOA limits are within 1 D. Thus, the two instruments are substantially equivalent, however, the CASIA SS-1000 had more consistent results.
Thinnest and central corneal thickness
TCT and CCT are important parameters for preoperative refractive surgery screening. In refractive surgery, based on the Munnerlyn formula [
12], every 12 μm of ablation depth will correct 1 D of the spherical equivalent for a single 6 mm ablation zone. Depending on the lasers and ablation zones, the average corneal ablation depth ranged from 12 to 17 μm per diopter [
12]. In addition, sufficient thickness of both flap and residual posterior stroma is required to prevent irregular astigmatism and to avoid diseases of corneal integrity and/or postoperative corneal ectasia. In general, a minimum of 110 μm flap will be created, leaving a minimum of 250 μm of residual posterior stroma. Thus, the accuracy of TCT and CCT are extremely important for thin corneas and high-myopia corrections, which require greater ablation depth. An overestimate of TCT and CCT during refractive surgery screening may result in postoperative complications. Therefore, an overestimate of 12–17 μm is considered clinically significant.
CASIA SS-1000 is 4x (repeatability ratio = 0.22–0.24) more precise than Pentacam when repeating TCT and CCT measurements using the same device, and 5x (repeatability ratio = 0.20) more precise than Pentacam when repeating TCT and CCT measurements using different devices.
The mean difference in CCT between CASIA SS-1000 and Pentacam of − 10.45 μm (~ 2% of average CCT for all participants) with LOA ranging 45 μm (− 33.75, 12.85) could be considered clinically significant. This difference may lead ophthalmologists to make a safer (more conservative) decision for refractive surgery. However, it may disqualify some patients who would be qualified if measured by Pentacam. The two measurements can be converted by the following equation: CASIA = 10.87 + 0.97 x Pentacam. For example, an eye measured 500 μm CCT by Pentacam can be converted to CASIA measurement as 490.87 μm. Therefore, decreasing the clinical difference in the values can be obtained. Similarly, TCT measurements can be converted by CASIA = 6.88 + 0.97 Pentacam. However, the decreased variability in measurements obtained with the CASIA SS-1000 results in more consistent clinical decisions.
In addition, after reviewing 94 papers published between 2000 and 2014, Rozema et al. reported that of the 14 instruments in which Pentacam was compared, five significantly overestimated CCT in normal eyes [
13]. These included TMS-5 (Tomey Corp, Nagoya, Japan, by 17 μm), Orbscan with AF (Bausch & Lomb, Claremont, CA, by 11
μm), Visante/Stratus (Carl Zeiss, Dublin, CA, by 14
μm), SL-OCT (Heidelberg Engineering, Heidelberg, Germany, by 16
μm), and specular microscopes (by 20
μm). Other studies showed similar results, with Pentacam overestimating by an average of 17
μm, compared to Visante OMNI (Carl Zeiss, Dublin, CA) (17.8 ± 11.3 μm) [
14], and Lenstar (Haag-Streit AG, Koeniz, Switzerland) (17.1 ± 8.5
μm) [
15].
Posterior corneal curvature
Recent studies have shown that posterior corneal curvature, especially maximal point of elevation over the best-fit reference image, was statistically significant for keratoconus [
16]. The ability to detect subclinical keratoconus earlier has important implications for improving refractive surgical screening to ensure that patients do not develop subsequent ectasia [
16]. In theory, flat and steep posterior corneal curvature (PKf and PKs) may provide useful information for more accurate toric IOL calculations. However, measured vs. assumed posterior corneal curvatures have shown no difference in IOL calculation accuracy [
17]. Our study showed that the CASIA SS-1000 underestimated the posterior corneal curvature comparing to the Pentacam. It is difficult to know what implications that an underestimate will have on screening for early keratoconus. The topographic morphologic distribution of the posterior corneal surface, specifically looking at the superior and inferior asymmetry within one topographic image, is a better early indicator of subclinical keratoconus than the raw absolute value of aggregate posterior corneal curvature being higher. Further studies are needed to analyze the topographic map of the posterior surface—and not just a solitary absolute value of the mean corneal curvature.
The absolute value of the posterior surface being smaller will affect the IOL calculation if the posterior corneal curvature value is used to calculate the total corneal power. However, recent studies using the Barret toric calculator showed that an estimated posterior curvature is just as accurate as a measured corneal curvature when looking at post-cataract refractive outcomes. As the Barrett formula is the current standard for toric IOL calculations, the difference in absolute value measurement of the posterior corneal surface between the Pentacam and CASIA will have little clinical difference in accuracy when correcting astigmatism, as the assumption has shown equivalent results to measured values. The anterior corneal surface measurements between the Pentacam and CASIA, which are more important in corneal measurement when performing IOL calculations, were not statistically different.
Peripheral corneal thickness
The corneal thickness and location data has less variability for the CASIA, compared to the Pentacam, in both repeatability and reproducibility. The CASIA corneal thickness measurements are also consistently lower in value than the Pentacam corneal thickness measurement in every point in the periphery: PCT2, PCT4, and PCT6. The pattern/distribution of corneal thickness (pachymetry map) and the location of the thinnest point in the corneal is important in planning for refractive surgery and detecting corneal diseases, such as keratoconus [
18]. Thus, less variability in both the corneal thickness measurements and location of thinnest corneal point are all very important clinical measurements for screening laser refractive surgery patients.