The aim of the present study was to investigate the efficacy and adverse effects of HCAG and FLAG re-induction chemotherapy in acute myeloid leukemia (AML) patients of low- and intermediate-risk groups following induction failure.
Methods
A total of 98 AML patients were enrolled. Among these subjects, 47 patients were treated with HCAG chemotherapy, while 51 patients were treated with FLAG chemotherapy.
Result
The complete remission (CR) and overall remission (OFF) were 24% and 38%, respectively in patients with HCAG induction chemotherapy, while the corresponding percentages were 28% and 42% in subject receiving FLAG chemotherapy. The median survival time of progress-free survival (PFS) was 29.8 (95% CI 23.749–35.851) months in the HCAG group and 30.8 (95% CI 21.728–39.872) months in the FLAG group (P = 0.620). A total of 42 patients in the HCAG group suffered from grade 4 hematological toxicity, while this adverse reaction was noted for all patients who were treated with FLAG chemotherapy (P = 0.023). A total of 19 cases indicated apparent nonhematological toxicity in the HCAG group, while only 40 (78.4%) were noted with these adverse reactions in the FLAG group (P = 0.000).
Conclusion
The HCAG regimen exhibited a similar effect compared with the FLAG regimen in low- and intermediate-risk groups, although the HCAG regimen significantly decreased the toxicity compared with that noted in the FLAG regimen group.
Hinweise
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Introduction
Acute myeloid leukemia (AML) is the most common form of acute leukemia. The disease is caused by infiltration of tumor cells in the blood, bone marrow, and other tissues [1, 2]. Although a variety of chemotherapeutic strategies have been developed, and the induction therapy response is successfully applied, a significant percentage of the patients (approximately 20–40% of the patients) fail to respond to therapy. The overall survival of the patients with induction therapy failure is significantly low compared with the percentage of patients with induction therapy who are in complete remission. Therefore, more effective therapies are required for the treatment of these patients. According to the National Comprehensive Cancer Network (NCCN) guidelines, the treatment strategy of the patients with induction failure may include targeted agents, combination chemotherapy and immunotherapy. Standard or high dose of cytarabine with other cytotoxic drugs such as fludarabine and granulocyte colony-stimulating factor, can be used. It has been reported that cytarabine plus fludarabine and granulocyte colony-stimulating factor (FLAG) form a salvage therapy in AML patients and can result in complete remission for a percentage range of 46–63% of the patients [3, 4]. Despite its high CR rate, FLAG can cause severe toxicity, such as hematological toxicity, mucositis, renal insufficiency, hepatic dysfunction and infections. The treatment-related mortality (TRM) of such cases was reported to range from 7 to 11.4% and the major cause of mortality was fungal infections [5, 6]. Therefore, effective and safe regimens are required to improve the survival of these patients. In previous studies, Wang T et al. compared the CR rate and toxicity between cytarabine, aclarubicin and granulocyte colony-stimulating factor (CAG) and FLAG groups. They showed that the CR rate and oral cavity toxicity were significantly different between these two groups. The CR rate and oral cavity toxicity of the FLAG group were higher than those of the CAG’s group [6]. Homoharringtonine (HHT) is one of the anti-tumor alkaloids isolated from the Chinese genus Cephalotaxus, and it is a periodic non-specific anti-tumor drug. HHT was found suitable for various types of AML treatment. The regimen containing HHT is currently widely used for the treatment of AML [7, 8]. Homoharringtonine, aclarubicin and cytarabine (HAA) regimen was reported to achieve 93.3% CR rate for AML (8; 21) as the first course of induction therapy [7]. In the present study, we used CAG add HHT as one new regimen and compared its efficacy and toxicity with those of the FLAG regimen.
Materials and methods
The present prospective study was conducted at the Lishui Municipal Central Hospital, which is a University-affiliated hospital with 1680 beds. A total of 98 AML patients with induction failure in low- and intermediate-risk groups were enrolled from May 2011 to July 2018. The research protocol was approved by the Medical Research and Ethics Committee of the hospital. Each patient signed the relevant informed comment. The inclusion criteria were as follows: (1) diagnosis of AML according to the classification of morphological, immunological, cytogenetic and molecular characteristics of white blood cells; (2) identification of risk status for low- and intermediate-risk groups according to the NCCN Guideline Version 2 issued in 2018; (3) initial treatment by standard dose of cytarabine and anthracyclines (standard 3 + 7 chemotherapy); (4) the presence of more than 20% of the treated tumor cells in the bone marrow of the patients during the 14–21 days follow-up period; (5) eastern Cooperative Oncology Group (ECOG) performance score less than 3; (6) normal cardiac, liver and renal functions. The exclusion criteria were the following: (1) acute promyelocytic leukemia, (2) serious organ dysfunction, (3) lactation or pregnancy. The patients of our present study were not allowed to attend other clinical trials. The endpoint was the number of patients with relapse.
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Treatment
The present study was a single blind, randomized controlled trial. According to the method of random allocation, all patients treated with HCAG or FLAG re-induction chemotherapy were randomized. The HCAG regimen consisted of the following drugs: low-dose cytarabine (10 mg/m2), at a 12-h dose cycle for days 1–14, aclarubicin (7 mg/m2/d) on days 1–8, G-CSF (200 μg/m2/d) on days 1–14 and HHT (1.5 mg/m2/day) on days 1–8. The FLAG regimen was as follows: fludarabine (30 mg/m2/day) on days 1–5, cytarabine (2 g/m2/day) on days 1–5, and G-CSF (300 μg/day) on days 0–5. The patients who achieved CR continued to receive consolidation therapy according to the NCCN guidelines. The patients with partial remission (PR) or no remission (NR) were administered second or high salvage therapy.
Data collection
The basement data were the following: age, gender, the percentage of BM blast cells following induction chemotherapy for 14–21 days, disease status, primary or secondary liver function, temperature measurement, chest computed tomography scan, glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) levels, renal function, creatinine and urea nitrogen levels, and the symptoms nausea, vomiting, and diarrhea.
Definitions and outcomes
AML risk stratification was assessed according to the 2018 NCCN Guidelines, based on validated cytogenetics and molecular abnormalities. The classification criteria of acute myeloid leukemia were as follows: (1) low risk: the cytogenetics including a core binding factor: inv(16), t(16;16), t(8;21). Normal cytogenetics combine with NPM1 mutation positive and FLT3-ITD negative or presence of FLT3-ITD with low allelic ratio (< 0.5), isolated biallelic CEBPA mutation. (2) Intermediate-risk: normal cytogenetics, t(9;11) and the presence of other non-defined cytogenetics with the exception of poor-risk cytogenetics, or molecular abnormalities including mutated NPM1 and FLT3-ITD with high allelic ratio(≥ 0.5), wild-type NPM1 without FLT3-ITD or with FLT3-ITD low allelic ratio, core binding factor with KIT mutation. A total of 243 cases experienced first induction failure from May 2011 to July 2018. According to this risk stratification, we retrospectively analyzed the cases before 2018, and concluded that a total of 98 cases were eligible for our study. CR status was evaluated in this aspects as follows: (1) no evidence indicates abnormal molecular profile and abnormal cytogenetics. (2) BM blast less than 5% following chemotherapy. (3) peripheral blood (PB): platelet count higher than 100 × 109/L or leukocyte count higher than 1 × 109/L without transfusion requirements. PR was evaluated as achieved in one of the following conditions: (1) BM blasts 5–25%. (2) A 50% or better decrease in BM blasts. (3) BM blasts < 5% but with Auer rods’ presence. The overall remission rate (ORR) was assessed in patients with CR and PR. The induction failure was defined as the failure to achieve ORR following standard 3 + 7 regimen treatment on days 14–21. The toxicity was evaluated according to the guidelines provided by the World Health Organization. The toxicity was divided to hematological and nonhematological toxicity, which comprised oral cavity toxicity, gastrointestinal complications, hepatic dysfunction, renal function impairment and pulmonary infection [9].
Observation time
The median observation time was 30.2 months (range of 21.728–39.872 months).
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Statistics
The data were analyzed using SPSS17. The comparison between HCAG and FLAG was analyzed by the t test. The univariate analysis of CR was analyzed using the χ2 test. The factors that exhibited significant differences (P < 0.01) from the initial analysis were analyzed by multivariate logistic regression. The PFS was estimated by the Kaplan–Meier method and the differences between the two groups were evaluated using the log-rank test. Cox proportional hazard models were constructed for PFS analysis. A stepwise selection method was used to determine the potential confounding covariates. The hazard ratio (HR) was estimated to assess the association of risk factors with PFS. A P value lower than 0.05 (P < 0.05) was considered for significant differences.
Results
Patient baseline characteristics
A total of 47 patients were enrolled in the HCAG group. This population contained 25 male and 22 female subjects. The median age was 48 ± 21.6 years. In contrast to the HCAG group, 51 patients were enrolled in the FLAG group, which included 32 male and 19 female subjects. The median age was 45 ± 20.7 years. The ECOG performance was used for patient evaluation, according to the following score: 0, 1, and 2. The HCAG group indicated that 18 (38.30%) cases exhibited abnormal chromosome number, while the number of the corresponding cases was 22 (43.14%) in the FLAG group. The BM blast at baseline in the HCAG and FLAG groups was estimated to 45 ± 22.9 and 45 ± 22.9, respectively. A total of 44 and 50 (93.6%, 98%) subjects were de novo AML cases in the HCAG and the FLAG groups, respectively. The remaining three and one (6.4% and 4.1%) subjects were secondary cases in the HCAG and FLAG groups, respectively (Table 1).
Table 1
Baseline characteristics
Parameters
HCAG treatment (N = 47)
FALG treatment (N = 51)
P value
Age (years)
48 ± 21.6
45 ± 20.7
0.071
Gender (male/female)
25/22
32/19
0.247
De novo or secondary
0.552
De novo (n/%)
44 (93.6%)
50 (98%)
Secondary (n/%)(n/%)
3 (6.4%)
1 (4.1%)
Abnormal chromosome (n/%)
18 (38.30%)
22 (43.14%)
0.749
WBC ≥ 50 × 109/L (n/%)
13 (27.7%)
11 (21.6%)
0.484
HGB ≤ 60 g/L (n/%)
9 (19.1%)
12 (23.5%)
0.598
PLT ≤ 30 × 109/L (n/%)
24 (51.1%)
30 (58.8%)
0.146
CEBPA (n/%)
1 (2.1%)
3 (5.9%)
0.669
c-kit (n/%)
3 (6.4%)
3 (5.9%)
1.000
NPM1 (n/%)
2 (4.3%)
9 (17.6%)
0.075
NPM1andFLT3-ITD (n/%)
4 (8.5%)
6 (11.8%)
0.843
BM blasts (%)
45 ± 22.9
48 ± 23.2
0.066
CR (n/%)
24 (51.1%)
28 (54.9%)
0.704
ORR (n/%)
38 (80.9%)
42 (82.4%)
0.848
Treatment-related mortality(TRM) (n/%)
0 (0)
4 (7.8%)
0.119
Clinical efficacy of the two treatments
51.1% (24/47) and 54.9% (28/51) of the patients exhibited CR in the HCAG and FLAG groups, respectively (P = 0.704) (Fig. 1). 80.9% (38/51) of the subjects achieved ORR in the HCAG group, while 82.4% (42/51) of these cases were noted in the FLAG group (P = 0.848) (Fig. 2). No significant differences were noted between the two groups. A total of four patients died for treatment relative adverse reaction and all of them came from FLAG group. The factors affecting CR were evaluated by the χ2 test. (Table 2). The parameters, nonhematological toxicity (P = 0.009), pulmonary infection (P = 0.000), platelet counts (P = 0.029) and BM blasts (P = 0.002) were associated with low possibility of achieving CR. The factors age, toxicity, platelet counts, BM blasts, pulmonary infection (P < 0.1) were analyzed in the multivariate model. The results indicated that the parameters age (P = 0.006), BM blasts (P = 0.014), pulmonary infection (P = 0.026) were independent predictive factors of CR (Table 3). The variables nonhematological toxicity (P = 0.022), abnormal chromosome number (P = 0.034), pulmonary infection (P = 0.001) and BM blasts (P = 0.014) were associated with ORR (Table 4). The factors age, nonhematological toxicity, abnormal chromosome number, pulmonary infection and BM blasts (P < 0.1) were analyzed by the multivariate model. The variables age (P = 0.036) and BM blasts (P = 0.038) indicated significant differences in the multivariate logistic regression analysis of ORR (Table 5). HCAG resulted in a median PFS of 29.8 (95% CI 23.749–35.851) months, while FLAG extended PFS to 30.8 (95% CI 21.728–39.872). The K–M curve and the log-rank tests were conducted to analyze PFS (Fig. 3). The data revealed no significant difference in PFS between the HCAG and the FLAG groups (P = 0.620). The univariate Cox regression analysis revealed that age (P = 0.000), nonhematological toxicity (P = 0.002), renal function impairment (P = 0.000), pulmonary infection (P = 0.002), hematological toxicity grade 4 (P = 0.004), CR (P = 0.000) and ORR (P = 0.000) were associated with PFS. The parameter age was associated with shorter PFS and CR, whereas the ORR group exhibited longer PFS (Table 6).
Fig. 1
Clinical efficacy after CLAG and FLAG therapies. 24/47 (51.1%) patients in HCAG group were evaluable for remission of which 51.1% achieved CR; 28/52 (54.9%) patients in FLAG group were evaluable for remission of which 54.9% achieved CR. No difference of CR between CLAG and FLAG was observed (P = 0.704)
Fig. 2
Clinical efficacy after CLAG and FLAG therapies. 38/47 (80.9%) patients in HCAG group were evaluable for remission of which 51.1% achieved ORR; 42/52 (82.4%) patients in the FLAG group were evaluable for remission of which 54.9% achieved ORR. No difference in CR was noted between CLAG and FLAG (P = 0.848)
Table 2
Univariate analysis of CR
Parameters
P value
Age
0.097
Male (vs. Female)
0.350
Abnormal chromosome (vs. normal chromosome)
0.914
Secondary disease (vs.de novo)
1.000
WBC
0.353
PLT
0.029
HGB
0.101
CEBPA gene positive
0.120
c-kit gene positive
1.000
NPM1 gene positive
0.408
Both NPM1 gene and FLT3-ITD gene positive
0.643
Hematological toxicity (grade 4)
0.888
Nonhematological toxicity
0.009
Oral cavity toxicity (> grade 2)
0.546
Pulmonary infection
0.000
Gastrointestinal complications
0.951
Hepatic dysfunction
0.227
Renal function impairment
0.371
BM blasts
0.002
Table 3
Multivariate logistic regression analysis of CR
Parameters
Univariate logistic regression
P value
OR
95% CI
Lower
Upper
Age
0.006
0.944
0.906
0.983
Toxicity
0.603
1.523
0.313
7.412
PLT
0.947
1.000
0.994
1.006
Pulmonary infection
0.026
0.164
0.034
0.804
BM blasts
0.045
0.963
0.928
0.999
Table 4
Univariate analysis of ORR
Parameters
P value
Age
0.098
Male (vs. female)
0.219
Hematological toxicity (grade 4)
0.585
Abnormal chromosome (vs. normal chromosome)
0.034
Secondary disease (vs.de novo)
1.000
WBC
0.182
PLT
0.148
HGB
0.600
CEBPA gene positive
0.579
c-kit gene positive
1.000
NPM1 gene positive
0.874
Both NPM1 gene and FLT3-ITD gene positive
0.704
Nonhematological toxicity
0.022
Oral cavity toxicity (> grade 2)
0.654
Gastrointestinal complications
0.910
Hepatic dysfunction
1.000
Renal function impairment
0.149
Pulmonary infection
0.001
BM blasts
0.014
Table 5
Multivariate logistic regression analysis of ORR
Parameters
Univariate logistic regression
P value
OR
95% CI
Lower
Upper
Age
0.036
0.943
0.892
0.996
Nonhematological toxicity
0.896
1.180
0.100
13.942
Abnormal chromosome (vs. normal chromosome)
0.125
2.795
0.751
10.403
Pulmonary infection
0.151
0.187
0.019
1.838
BM blasts
0.038
0.961
0.925
0.998
Fig. 3
Extended PFS by HCAG and FLAG treatments. K–M curve analysis of accumulating PFS. HCAG therapy achieved median PFS of 29.8 (95% CI 23.749–35.851) months, whereas FLAG therapy achieved median OS of 30.8 (95% CI 21.728–39.872) months. No differences were noted with regard to the PFS. The comparison of the PFS between the groups was determined by K–M curves and the log-rank test. A P value less than 0.05 (P < 0.05) was considered for significant differences
Table 6
Univariate and multivariate Cox’s proportional hazard regression analysis of factors affecting PFS
Parameters
Univariate Cox regression
Multivariate Cox regression
P value
HR
95% CI
P value
HR
95% CI
Lower
Higher
Lower
Higher
Age
0.000
1.094
1.059
1.130
0.000
1.152
1.099
1.207
Male (vs. female)
0.957
1.016
0.581
1.777
0.533
0.806
0.409
1.588
Nonhematological toxicity
0.002
2.564
1.400
4.697
0.783
1.254
0.251
6.252
Oral cavity toxicity (> grade 2)
0.441
0.787
0.429
1.446
0.368
0.674
0.286
1.591
Gastrointestinal complications
0.406
1.380
0.646
2.946
0.176
2.428
0.672
8.775
Hepatic dysfunction
0.143
1.902
0.805
4.492
0.061
2.771
0.953
8.061
Renal function impairment
0.000
4.582
1.975
10.632
0.167
2.395
0.695
8.252
Pulmonary infection
0.002
2.453
1.389
4.333
0.119
3.363
0.731
15.464
Hematological toxicity (grade 4)
0.004
0.244
0.093
0.636
0.198
0.433
0.121
1.548
Abnormal chromosome
0.356
0.766
0.435
1.350
0.634
1.203
0.563
2.569
Secondary disease (vs.de novo)
0.357
0.394
0.054
2.865
0.096
0.149
0.016
1.403
WBC
0.911
1.001
0.987
1.015
0.248
1.014
0.990
1.038
HGB
0.191
0.992
0.980
1.004
0.908
1.001
0.981
1.021
PLT
0.749
0.999
0.995
1.004
0.187
0.997
0.993
1.001
CEBPA gene positive
0.167
0.247
0.034
1.797
0.884
0.846
0.089
8.079
c-kit gene positive
0.634
0.753
0.234
2.420
0.820
1.173
0.298
4.618
NPM1 gene positive
0.596
0.805
0.362
1.791
0.296
2.588
0.436
15.377
Both NPM1 gene and FLT3-ITD gene positive
0.883
0.926
0.332
2.581
0.426
0.422
0.050
3.535
BM blasts
0.424
1.007
0.990
1.023
0.111
1.019
0.996
1.043
CR
0.000
0.152
0.081
0.284
0.001
0.235
0.098
0.562
ORR
0.000
0.153
0.080
0.291
0.001
0.177
0.061
0.512
×
×
×
Toxicity
A total of 42 patients in the HCAG group experienced grade 4 hematological toxicity compared with all the patients in the FLAG group that did not exhibit this symptom (P = 0.023). A total of 19 cases indicated considerable nonhematological toxicity in the HCAG group, while 40 patients (78.4%) exhibited this feature in the FLAG group (P = 0.000). No significant differences were noted between the two groups with regard to the hematological or nonhematological toxicities. The toxicity of the HCAG group was lower than that of the FLAG group. The groups were divided into five subgroups based on the difference in nonhematological toxicity. The groups were as follows: oral cavity toxicity, hepatic dysfunction, renal impairment, pulmonary infection and gastrointestinal disorders. A total of eight (17.02%) cases exhibited oral cavity toxicity higher than grade 2 in the HCAG group, whereas 27 (52.94%) cases exhibited this type of toxicity in the FLAG group (P = 0.000). A total of ten patients demonstrated hepatic dysfunction and were divided to two (4.3%) cases in the HCAG group and eight (15.7%) cases in the FLAG group (P = 0.117). Renal impairment occurred in four (8.5%) patients of the HCAG group and 5 (9.8%) patients of the FLAG group (P = 1.000). A total of 47 subjects exhibited pulmonary infection following chemotherapy, of which 15 (31.9%) cases were included in the HCAG group, and 32 (62.7%) in the FLAG group (P = 0.002). A total of 13 patients experienced gastrointestinal disorders and were divided to 6 (12.8%) subjects in the HCAG group and 7 (13.7%) in the FLAG group (P = 0.889) (Table 7).
Table 7
Comparison of two groups with toxicity
Toxicity
HCAG treatment (N = 47)
FALG treatment (N = 51)
P value
Hematological toxicity (grade 4)
42
51
0.023
Nonhematological toxicity
19 (40.4%)
40 (78.4%)
0.000
Oral cavity toxicity (≥ grade 2)
8 (17.02%)
27 (52.94%)
0.000
Gastrointestinal complications (n/%)
6 (12.8%)
7 (13.7%)
0.889
Hepatic dysfunction (n/%)
2 (4.3%)
8 (15.7%)
0.117
Renal function impairment (n/%)
4 (8.5%)
5 (9.8%)
1
Pulmonary infection (n/%)
15 (31.9%)
32 (62.7%)
0.002
Discussion
According to the NCCN guideline, FLAG therapy is considered a salvage therapy for patients of low and intermediate risk for AML following initial induction failure. Various studies have indicated that FLAG can achieve approximately 50% CR rate in treating patients with refractory or relapsed AML. In addition, the treatment-related mortality is estimated to 11.4%. Therefore, the toxicity caused by FLAG chemotherapy is substantial. Due to these disadvantages, several alternative regimens of FLAG chemotherapy were used in clinical studies.
Homoharringtonine (HHT) is an alkaloid derived from trees of the genus Cephalotaxus and has demonstrated significant efficacy for the treatment of acute myeloid leukemia (AML). This compound has been widely used in China. The mechanism of action of HHT involves incorporation of the compound into the DNA that causes inhibition of DNA synthesis and induces leukemic cell apoptosis. The decreased expression of the apoptotic-related protein survivin and the down-regulation of the expression of telomerase were also associated with the induction of apoptosis in leukemia cells [10‐12]. Moreover, the inhibition of the protein synthesis caused by HHT prevented binding of RNA substrates and aminoacyl-tRNAs to the 60 s ribosomal subunit and inhibited the elongation phase of translation [13]. In addition, HHT can induce leukemic cell differentiation. Therefore, the mechanism of action of HHT is mainly based on the inhibition of protein synthesis, which in turn induces leukemic cell differentiation, inhibits their proliferation, and promotes apoptosis [14‐17]. Despite these findings, the exact mechanism remains unclear. HHT can cause synergistic augmentation of therapeutic efficacy towards AML with cytarabine (Ara-c) and aclarubicin (Acla) [15]. Wang et al. demonstrated that HHT had a synergistic relationship with aclarubicin’s cytotoxicity in vitro and in vivo [17]. G-CSF is a granulocyle colony-stimulating factor that promotes granulocytic lineage cells from the bone marrow to the peripheral blood and can be used to enhance drug leukemia cell killing. It can also be used to induce G0–G1 phase transition and enhance the anti-leukemic effect of HHT [18]. Previous studies have shown that the CR rate and oral cavity toxicity were significantly different between the FLAG group and CAG group. The CR rate and oral cavity toxicity of the FLAG group were both higher than those of the CAG group [6]. The present study aimed to use a combined treatment of CAG and HHT to produce a new regimen. This regimen was then compared with the FLAG regimen in terms of efficacy and tolerability for AML patients receiving salvage therapy.
The present study compared the efficacy and toxicity of HCAG and FLAG chemotherapy in low- and intermediate-risk groups of AML patients with first induction failure. The results can be summarized as follows: (1) efficacy: no difference was noted in the CR rate between the HCAG and the FALG groups. Specifically, the HCAG patients achieved 51.1% CR rate, while the FLAG patients 54.9% (P = 0.704) CR rate. A similar performance was noted with regard to ORR for the two treatments. The patients exhibited 80.9% ORR in the HCAG and 82.4% ORR in the FLAG groups, respectively (P = 0.848). The PFS exhibited no difference between these two groups and the HCAG group revealed a median PFS of 29.8 (95% CI 23.749-35.851) months, while the FLAG group a median PFS of 30.8 (95% CI 21.728–39.872) (P = 0.620). Therefore, it can be assumed that no difference was observed in efficacy between the two groups. (2) Toxicity: the HCAG group exhibited lower toxicity than the FLAG group regardless of the grade of hematological toxicity (grade 4) (P = 0.023) or the presence of nonhematological toxicity (P = 0.009). A total of four cases did not survive in the FALG group while all subjects survived in the HCAG group. Although no significant difference was noted between the two groups, the results suggest that these types of treatment should be handled with caution. In the subgroup of the nonhematological toxicity cases, the parameter incidence rate of oral cavity toxicity (higher than 2), was different between the two groups (P = 0.000). In addition, the incidence of pulmonary infection (P = 0.000) in the FLAG group was higher than that of the HCAG group. The parameters gastrointestinal complications (P = 0.887), hepatic dysfunction (P = 0.778) and renal function impairment (P = 1.000) were evaluated and no difference was noted between the two groups. This result may be due the low number of cases. Following clinical treatment, non-hematologic toxicity was acceptable and routine supportive therapy was sufficient to control the symptoms.
Based on these results, it can be deduced that the HCAG regimen is as effective as the FLAG regimen and that it is more easily tolerated than the FLAG regimen, which suggests that it may be an optimal selection for low and intermediate-risk AML patients with first induction failure (3). The biochemical factors that affected CR, ORR, and PFS were analyzed as follows: platelet counts (P = 0.029) and BM blasts (P = 0.002). These were considered to affect CR as determined by univariate logistic regression analysis. However, the variables age (P = 0.006), BM blasts (P = 0.014), and pulmonary infection (P = 0.026) were independent predictive factors for low CR. The probable predictive factors for ORR were the following: nonhematological toxicity (P = 0.022), abnormal chromosome number (P = 0.034), pulmonary infection (P = 0.001), and BM blasts (P = 0.014). The factors age, nonhematological toxicity, abnormal chromosome number, pulmonary infection, and BM blasts (P < 0.1) were analyzed by the multivariate model. The parameters age (P = 0.036) and BM blasts (P = 0.038) indicated statistically significant differences in the multivariate logistic regression analysis of ORR. This finding is similar to that noted by Xu et al. who reported that age and BM blast were independent predictive factors for CR, and that BM was an independent predictive factor for low risk of ORR in refractory or relapsed AML patients [19]. Moreover, our study indicated that age was associated with shorter PFS and that CR and ORR were associated with longer PFS. These findings have been verified by several studies.
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The present study has several limitations that are summarized as follows: (1) this was a single-center cohort study; (2) the sample size was relatively small. Since there were just 31 patients have molecular abnormalities, it was not clear whether abnormal molecular analyses and stratification based age could affect the clinical efficacy of the two treatments. (3) The first induction regimens including IA, HA, DA, MA, may have influenced the reliability of the results.
In conclusion, HCAG was equally effective to FLAG in re-induction chemotherapy, whereas it exhibited optimal safety and tolerability in low and intermediate-risk AML patients with first induction failure. HCAG regimen can be considered as a new salvage therapy used in low and intermediate-risk AML patients with first induction failure.
Compliance with ethical standards
Conflict of interest
The authors have declared no conflicts of interest.
Ethical approval
All procedures performed in the study were in accordance with the ethical standards of the Institutional and National Research Committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
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Informed consent
Informed consent for the treatment of HCAG regimen and FLAG regimen proposed was obtained from all individual participants included in the study.
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Nun gibt es auch Resultate zum Gesamtüberleben: Eine adjuvante Pembrolizumab-Therapie konnte in einer Phase-3-Studie das Leben von Menschen mit Nierenzellkarzinom deutlich verlängern. Die Sterberate war im Vergleich zu Placebo um 38% geringer.
Das Risiko für Rezidiv oder Tod von Patienten und Patientinnen mit reseziertem ALK-positivem NSCLC ist unter einer adjuvanten Therapie mit dem Tyrosinkinase-Inhibitor Alectinib signifikant geringer als unter platinbasierter Chemotherapie.
Patienten, die zur Behandlung ihres Prostatakarzinoms eine Androgendeprivationstherapie erhalten, entwickeln nicht selten eine Anämie. Wer ältere Patienten internistisch mitbetreut, sollte auf diese Nebenwirkung achten.
Müssen sich Schwangere einer Krebstherapie unterziehen, rufen Immuncheckpointinhibitoren offenbar nicht mehr unerwünschte Wirkungen hervor als andere Mittel gegen Krebs.
Update Onkologie
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