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Erschienen in: Archives of Virology 9/2016

01.07.2016 | Annotated Sequence Record

Complete genome sequences of a putative new alphapartitivirus detected in Rosa spp.

verfasst von: James Phelan, Delano James

Erschienen in: Archives of Virology | Ausgabe 9/2016

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Abstract

A putative new alphapartitivirus was detected by next-generation sequencing (NGS) in Rosa spp. and identified as rose partitivirus isolate Phyllis Bide (RoPV-PB). The virus is bipartite with a dsRNA1 fragment (1937 bp) encoding a putative RdRp and a dsRNA2 fragment (1811 bp) encoding the putative CP subunit of the virus. dsRNA1 of RoPV-BP is closely related to Vicia faba partitivirus 1, with identities of 67 % and 72 % for the nucleotide (nt) and deduced amino acid (aa) sequences, respectively. In NGS analysis of RoPV-BP, coverage was uneven across both dsRNA fragments, with GC/AT content appearing to be a major determinant of depth of coverage.
Literatur
1.
Zurück zum Zitat Adams IP, Glover RH, Monger WA, Mumford R, Jackeviciene E, Navalinskiene M, Samuitiene M, Boonham N (2009) Next-generation sequencing and metagenomics analysis: a universal diagnostic tool in plant virology. Mol Plant Pathol 10:537–545CrossRefPubMed Adams IP, Glover RH, Monger WA, Mumford R, Jackeviciene E, Navalinskiene M, Samuitiene M, Boonham N (2009) Next-generation sequencing and metagenomics analysis: a universal diagnostic tool in plant virology. Mol Plant Pathol 10:537–545CrossRefPubMed
2.
Zurück zum Zitat Ahmad I, Dole JM, Khan A, Qasim M, Ahmad T, Khan AS (2010) Present status and future prospects of cut rose production in Punjab, Pakistan. HortTechnol 20:1010–1015 Ahmad I, Dole JM, Khan A, Qasim M, Ahmad T, Khan AS (2010) Present status and future prospects of cut rose production in Punjab, Pakistan. HortTechnol 20:1010–1015
3.
Zurück zum Zitat Attoui H, Billoir F, Cantaloube JF, Biagini P, de Micco P, Lamballerie X (2000) Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89:147–158CrossRefPubMed Attoui H, Billoir F, Cantaloube JF, Biagini P, de Micco P, Lamballerie X (2000) Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89:147–158CrossRefPubMed
4.
Zurück zum Zitat Byrne DH (2015) Advances in rose breeding and genetics in North America. Acta Hortic 1064:89–98CrossRef Byrne DH (2015) Advances in rose breeding and genetics in North America. Acta Hortic 1064:89–98CrossRef
5.
Zurück zum Zitat Debener T, Byrne DH (2014) Disease resistance breeding in rose: current status and potential of biotechnological tools. Plant Sci 228:107–117CrossRefPubMed Debener T, Byrne DH (2014) Disease resistance breeding in rose: current status and potential of biotechnological tools. Plant Sci 228:107–117CrossRefPubMed
6.
Zurück zum Zitat James D, Varga A (2012) Sequence analysis of RNA1 of lilac leaf chlorosis virus supports a close relationship to subgroup 3 ilarviruses. Arch Virol 157:203–206CrossRefPubMed James D, Varga A (2012) Sequence analysis of RNA1 of lilac leaf chlorosis virus supports a close relationship to subgroup 3 ilarviruses. Arch Virol 157:203–206CrossRefPubMed
7.
Zurück zum Zitat Koonin EV (1991) The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol 72:2197–2206CrossRefPubMed Koonin EV (1991) The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol 72:2197–2206CrossRefPubMed
8.
Zurück zum Zitat Mammedov TG, Pienaar E, Whitney SE, TerMaat JR, Carvill G, Goliath R, Subramanian A, Viljoen HJ (2008) A fundamental study of the PCR amplification of GC-rich DNA templates. Comput Biol Chem 32:452–457CrossRefPubMedCentral Mammedov TG, Pienaar E, Whitney SE, TerMaat JR, Carvill G, Goliath R, Subramanian A, Viljoen HJ (2008) A fundamental study of the PCR amplification of GC-rich DNA templates. Comput Biol Chem 32:452–457CrossRefPubMedCentral
9.
Zurück zum Zitat Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Jiang D, Suzuki N (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188:128–141CrossRefPubMed Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Jiang D, Suzuki N (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188:128–141CrossRefPubMed
11.
Zurück zum Zitat Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542CrossRefPubMedPubMedCentral Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542CrossRefPubMedPubMedCentral
12.
Zurück zum Zitat Tzanetakis IE, Martin RR (2008) A new method for extraction of double-stranded RNA from plants. J Virol Methods 149:167–170CrossRefPubMed Tzanetakis IE, Martin RR (2008) A new method for extraction of double-stranded RNA from plants. J Virol Methods 149:167–170CrossRefPubMed
Metadaten
Titel
Complete genome sequences of a putative new alphapartitivirus detected in Rosa spp.
verfasst von
James Phelan
Delano James
Publikationsdatum
01.07.2016
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 9/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-2929-1

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