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07.02.2017 | Original Article

Constituents of leaves from Bauhinia curvula Benth. exert gastroprotective activity in rodents: role of quercitrin and kaempferol

verfasst von: Ana Paula Beber, Priscila de Souza, Thaise Boeing, Lincon Bordignon Somensi, Luísa Nathália Bolda Mariano, Benhur Judah Cury, Ligia Moura Burci, Cristiane Bezerra da Silva, Euclésio Simionatto, Sérgio Faloni de Andrade, Luísa Mota da Silva

Erschienen in: Inflammopharmacology | Ausgabe 2/2018

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Abstract

The Bauhinia genus is known as “Pata-de-Vaca” and a wide variety of these species are used in Brazilian folk medicine due to their gastroprotective properties. This study aimed to investigate the antiulcer efficacy of the hydroalcoholic extract from B. curvula (HEBC) leaves, as well as its semi-purified fraction (SPFr) and the contribution of their phytochemicals constituents for this effect. For that, ethanol 60%/HCl 0.3 M- and indomethacin-induced gastric ulcer were performed in rodents. Gastric ulcerated tissues were processed for histological, histochemical and biochemical analysis. The oral treatment with HEBC and SPFr decreased the gastric ulcer induced by ethanol/HCl in mice and by indomethacin (only HEBC) in rats. The gastroprotective effect of HEBC was abolished in mice pretreated with Nω-Nitro-l-arginine methyl ester, N-Ethylmaleimide, glibenclamide or indomethacin. Both HEBC and SPFr reduced myeloperoxidase activity in parallel with a decrease of lipoperoxides content at the site of the lesion. On the other hand, HEBC did not alter volume, pH, total acidity or pepsin activity of acid gastric secretion in rats, and neither inhibited the in vitro H(+),K(+)-ATPase activity. Additionally, the compounds identified and isolated from the SPFr, the flavonoids quercitrin (65%) and kaempferol (35%), were able to diminish the extent of ulcerated area induced by both ethanol/HCl and indomethacin. Taking together, these findings show that B. curvula extracts present gastroprotective effect, mainly explained by the presence of flavonoids quercitrin and kaempferol, which may possibly improve the defensive factors of gastric mucosa.

Abdelwahab SI, Taha MM, Abdulla MA, Nordin N, Hadi AH, Mohan S, Jayapalan JJ, Hashim OH (2013) Gastroprotective mechanism of Bauhinia thonningii Schum. J Ethnopharmacol 148(1):277–286CrossRefPubMed

Ahmad A, Gupta G, Afzal M, Kazmi I, Anwar F (2013) Antiulcer and antioxidant activities of a new steroid from Morus alba. Life Sci 92(3):202–210CrossRefPubMed

Alirezaei M, Dezfoulian O, Neamati S, Rashidipour M, Tanideh N, Kheradmand A (2012) Oleuropein prevents ethanol-induced gastric ulcers via elevation of antioxidant enzyme activities in rats. J Physiol Biochem 68(4):583–592CrossRefPubMed

Anson ML (1938) The estimation of pepsin, trypsin, papain, and Cathepsin with hemoglobin. J Gen Physiol 22:79–89CrossRefPubMedPubMedCentral

Arnous A, Makris DP, Kefalas P (2001) Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. J Agric Food Chem 49:5736–5742CrossRefPubMed

Arvouet-Grand A, Vennat B, Pourrat A, Legret P (1994) Standardization of propolis extract and identification of principal constituents. J Pharm Belg 49(6):462–468PubMed

Baumer M (1983) Notes on trees and shrubs in arid and semi-arid regions. Food and Agriculture Organization of the United Nations.

Benjamim CF, Ferreira SH, Cunha FQ (2000) Role of nitric oxide in the failure of neutrophil migration in sepsis. J Infect Dis 182(1):214–223CrossRefPubMed

Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200CrossRef

Boeing T, da Silva LM, Somensi LB, Cury BJ, Michels Costa AP, Petreanu M, Niero R, de Andrade SF (2016) Antiulcer mechanisms of Vernonia condensata Baker: a medicinal plant used in the treatment of gastritis and gastric ulcer. J Ethnopharmacol 184:196–207CrossRefPubMed

Bornschein J, Malfertheiner P (2014) Helicobacter pylori and gastric cancer. Dig Dis 32(3):249–264CrossRefPubMed

Brito A, Ramirez JE, Areche C, Sepúlveda B, Simirgiotis MJ (2014) HPLC-UV-MS profiles of phenolic compounds and antioxidant activity of fruits from three citrus species consumed in Northern Chile. Molecules 19:17400–17421CrossRefPubMed

Brzozowski T, Konturek PC, Sliwowski Z, Kwiecien S, Drozdowicz D, Pawlik M, Mach K, Konturek SJ, Pawlik WW (2006) Interaction of nonsteroidal anti-inflammatory drugs (NSAID) with Helicobacter pylori in the stomach of humans and experimental animals. J Physiol Pharmacol 57:67–79PubMed

Carlson M, Raab Y, Seveus L, Xu S, Hallgren R, Venge P (2002) Human neutrophil lipocalin is a unique marker of neutrophil inflammation in ulcerative colitis and proctitis. Gut 50:501–506CrossRefPubMedPubMedCentral

Chen FA, Wu AB, Chen CY (2004) The influence of treatments on the free radical scavenging activity of burdock and variations of its active. Food Chem 86:479–484CrossRef

Chen H, Olatunji OJ, Zhou Y (2016) Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice. J Nat Med 70:610–619CrossRefPubMed

da Silva LM, Boeing T, Somensi LB, Cury BJ, Steimbach VM, Silveria AC, Niero R, Cechinel Filho V, Santin JR, de Andrade SF (2015) Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: in vitro and in vivo studies. J Ethnopharmacol 175:75–85CrossRefPubMed

Dacha S, Razvi M, Massaad J, Cai Q, Wehbi M (2015) Hypergastrinemia. Gastroenterology 3:201–208

Dal Secco D, Moreira AP, Freitas A, Silva JS, Rossi MA, Ferreira SH, Cunha FQ (2006) Nitric oxide inhibits neutrophil migration by a mechanism dependent on ICAM-1: role of soluble guanylate cyclase. Nitric Oxide 15(1):77–86CrossRef

de Barros M, da Silva LM, Boeing T, Somensi LB, Cury BJ, Burci LM, Santin JR, Andrade SF, Monache FD, Cechinel-Filho V (2016) Pharmacological reports about gastroprotective effects of methanolic extract from leaves of Solidago chilensis (Brazilian arnica) and its components quercitrin and afzelin in rodents. Naunyn Schmiedebergs Arch Pharmacol 389(4):403–417CrossRefPubMed

Eom CS, Lee SS (2011) Risk of fracture and pneumonia from acid suppressive drugs. World J Methodol 1:15–21CrossRefPubMedPubMedCentral

Gupta MP (1995) 270 Plantas medicinales ibero-americanas; Talleres de Editorial Presencia, Santafé de Bogotá: Colombia.

Hisam EE, Zakaria ZA, Mohtaruddin N, Rofiee MS, Hamid HA, Othman F (2012) Antiulcer activity of the chloroform extract of Bauhinia purpurea leaf. Pharm Biol 50(12):1498–1507CrossRefPubMed

Kangwan N, Park JM, Kim EH, Hahm KB (2014) Quality of healing of gastric ulcers: natural products beyond acid suppression. World J Gastrointest Pathophysiol 5(1):40–47CrossRefPubMedPubMedCentral

Kubo K, Uehara H, Kubota T, Nozu T, Moruya M, Watanabe Y, Shoji E, Santos SB, Harada K, Kongo Y (1995) Effects of ranitidine on gastric vesicles containing H+, K+-ATPase adenosine triphosphatase in rats. Scand J Gastroenterol 30:944–951CrossRefPubMed

Li X, Jiang Q, Wang T, Liu J, Chen D (2016) Comparison of the antioxidant effects of quercitrin and isoquercitrin: understanding the role of the 6″-OH Group. Molecules 1921(9):E1246CrossRef

Lichtenberger LM (1999) Gastroduodenal mucosal defense. Curr Opin Gastroenterol 15(6):463–472CrossRefPubMed

Liu HP, Shi XF, Zhang YC, Li ZX, Zhang L, Wang ZY (2011) Quantitative analysis of quercetin in Euphorbia helioscopia L by RP-HPLC. Cell Biochem Biophys 61(1):59–64CrossRefPubMed

Lu H, Graham DY (2006) New development in the mechanistic understanding of peptic ulcer diseases. Drug Discov Today Dis Mech 3(4):431–437CrossRef

Lundberg JO, Weitzberg E, Gladwin MT (2008) The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nat Rev Drug Discov 7(2):156–167CrossRefPubMed

Marhuenda E, Martin MJ, Alarcon De La Lastra C (1993) Antiulcerogenic activity of aescine in different experimental models. Phytother Res 7(1):13–16CrossRef

Mariano LN, da Silva LM, de Souza P, Boeing T, Somensi LB, Bonomini TJ, Delle Monache F, Cechinel Filho V, de Andrade SF, Niero R (2016) Gastroprotective xanthones isolated from Garcinia achachairu: study on mucosal defensive factors and H(+), K(+)-ATPase activity. Chem Biol Interact 258:30–39CrossRefPubMed

Matsuda H, Li Y, Yoshikawa M (1999) Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulphydryls, and prostaglandins in gastroprotection by mormodin Ic, na oleanolic acid oligoglycoside, on ethanol-induced gastric mucosal lesion in rats. Life Sci 65:27–32CrossRef

Medeiros JV, Gadelha GG, Lima SJ, Garcia JA, Soares PM, Santos AA, Brito GA, Ribeiro RA, Souza MH (2008) Role of the NO/cGMP/K(ATP) pathway in the protective effects of sildenafil against ethanol-induced gastric damage in rats. Br J Pharmacol 153:721–727CrossRefPubMed

Morimoto Y, Shimohara K, Oshima S, Sukamoto T (1991) Effects of the new anti-ulcer agent KB-5492 on experimental gastric mucosal lesions and gastric mucosal defensive factors, as compared to those of teprenone and cimetidine. J Pharmacol 57(4):495–505

Mózsik G (2010) Gastric cytoprotection 30 years after its discovery by André Robert: a personal perspective. Inflammopharmacology 5:209–221CrossRef

Mwase WF, Mvula T (2011) Effect of seed size and pre-treatment methods of Bauhinia thonningii Schum. on germination and seedling growth. Afr J Biotechnol 10:5143–5148

Nwafor PA, Okwuasaba FK, Binda LG (2000) Antidiarrhoeal and antiulcerogenic effects of methanolic extract of Asparagus pubescens root in rats. J Ethnopharmacol 72(3):421–427CrossRefPubMed

Peskar BM, Ehrlich K, Peskar BA (2002) Role of ATP-sensitive potassium channels in prostaglandin-mediated gastroprotection in the rat. J Pharmacol Exp Ther 301:969–974CrossRefPubMed

Robert A (1981) Current history of cytoprotection. Prostaglandins 21:89–96CrossRefPubMed

Robert A, Nezamis JE, Lancaster C, Hanchar AJ (1979) Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology 77(3):433–443PubMed

Rozza AL, Cesar DA, Pieroni LG, Saldanha LL, Dokkedal AL, De-Faria FM, Souza-Brito AR, Vilegas W, Takahira RK, Pellizzon CH (2015) Antiulcerogenic activity and toxicity of Bauhinia holophylla hydroalcoholic extract. Evid Based Complement Alternat Med 2015:439506CrossRefPubMedPubMedCentral

Shay H, Komarov SA, Fels SS, Marenze D, Grunstein M, Siplet H (1945) A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 5:43–61

Sheen E, Triadafilopoulos G (2011) Adverse effects of long-term proton pump inhibitor therapy. Dig Dis Sci 56:931–950CrossRefPubMed

Skiba MA, Szendzielorz K, Mazur B, Król W (2016) The inhibitory effect of flavonoids on interleukin-8 release by human gastric adenocarcinoma (AGS) cells infected with cag PAI (+) Helicobacter pylori. Cent Eur J Immunol 41(3):229–235CrossRefPubMedPubMedCentral

Suchal K, Malik S, Gamad N, Malhotra RK, Goyal SN, Bhatia J, Arya DS (2016) Kaempeferol protects against oxidative stress and apoptotic damage in experimental model of isoproterenol-induced cardiac toxicity in rats. Phytomedicine 23(12):1401–1408CrossRefPubMed

Suzuki H, Matsuzaki J, Hibi T (2011) Ghrelin and oxidative stress in gastrointestinal tract. J Clin Biochem Nutr 48:122–125CrossRefPubMed

Szabo S (1984) Peptides, sulfhydryls, and glucocorticoids in gastric mucosal defense: coincidence or connection? Gastroenterology 87:228–229PubMed

Tarnawski AS (2005) Cellular and molecular mechanisms of gastrointestinal ulcer healing. Dig Dis Sci 50:24–33CrossRef

Teske M, Trentini AMM (1995) Compêndio de Fitoterapia. Herbarium Laboratório Botânico, Curitiba

Thomson AB, Sauve MD, Kassam N, Kamitakahara H (2010) Safety of the long-term use of proton pump inhibitors. World J Gastroenterol 16:2323–2330CrossRefPubMedPubMedCentral

Veeresham C (2012) Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res 3(4):200–201CrossRefPubMedPubMedCentral

Zakaria ZA, Abdul Hisam EE, Rofiee MS, Norhafizah M, Somchit MN, Teh LK, Salleh MZ (2011) In vivo antiulcer activity of the aqueous extract of Bauhinia purpurea leaf. J Ethnopharmacol 137(2):1047–1054CrossRefPubMed

Zakaria ZA, Abdul Hisam EE, Norhafizah M, Rofiee MS, Othman F, Hasiah AH, Vasudevan M (2012) Methanol extract of Bauhinia purpurea leaf possesses anti-ulcer activity. Med Princ Pract 21(5):476–482CrossRefPubMed

Titel: Constituents of leaves from Bauhinia curvula Benth. exert gastroprotective activity in rodents: role of quercitrin and kaempferol
verfasst von: Ana Paula Beber
Priscila de Souza
Thaise Boeing
Lincon Bordignon Somensi
Luísa Nathália Bolda Mariano
Benhur Judah Cury
Ligia Moura Burci
Cristiane Bezerra da Silva
Euclésio Simionatto
Sérgio Faloni de Andrade
Luísa Mota da Silva
Publikationsdatum: 07.02.2017
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 2/2018
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0313-8

Springer Medizin

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