Background
Methods
Tumor tissue samples and patients
Negative pAKT | Positive pAKT | |||||
---|---|---|---|---|---|---|
Total | n | % | n | % |
P
| |
NO, patients
|
36
|
22
|
61
|
14
|
39
| |
Sex
|
Male
|
10
|
45
|
6
|
43
|
0.577
|
Female
|
12
|
55
|
8
|
57
| ||
Age
|
< 60 years
|
15
|
68
|
10
|
71
|
0.569
|
≥ 60 years
|
7
|
32
|
4
|
29
| ||
Median [range]
|
56 [30-72]
|
52 [34-68]
|
0.575
| |||
Lactate dehydrogenase
|
N
|
19
|
86
|
8
|
57
|
0.03
|
>N
|
2
|
9
|
6
|
43
| ||
Unknown
|
1
|
5
|
0
|
0
| ||
Performance status
|
0
|
7
|
32
|
2
|
14
|
0.045
|
1
|
0
|
0
|
3
|
22
| ||
Unknown
|
15
|
68
|
9
|
64
| ||
B symptoms
|
0
|
5
|
23
|
4
|
29
|
0.601
|
1
|
4
|
18
|
4
|
29
| ||
Unknown
|
13
|
59
|
6
|
42
| ||
Ann Arabor stage
|
I-II
|
7
|
32
|
2
|
14
|
0.254
|
III-IV
|
15
|
68
|
11
|
79
| ||
Unknown
|
0
|
0
|
1
|
7
| ||
Adenopathy
|
0-3
|
14
|
64
|
7
|
50
|
0.282
|
4 or more
|
6
|
27
|
6
|
43
| ||
Unknown
|
2
|
9
|
1
|
7
| ||
Hemoglobin
|
≥ 12 g/dl
|
13
|
59
|
9
|
64
|
0.684
|
< 12 g/dl
|
3
|
14
|
2
|
14
| ||
Unknown
|
6
|
27
|
3
|
22
| ||
β2 microglobulin
|
N
|
9
|
41
|
5
|
36
|
0.306
|
> N
|
4
|
18
|
5
|
36
| ||
Unknown
|
9
|
41
|
4
|
28
| ||
Bone marrow involvement
|
0
|
9
|
41
|
7
|
50
|
0.58
|
1
|
10
|
45
|
7
|
50
| ||
Unknown
|
3
|
14
|
0
|
0
| ||
FLIPI
|
0-1
|
3
|
14
|
1
|
7
|
0.390
|
2
|
7
|
32
|
4
|
29
| ||
≥ 3
|
4
|
18
|
6
|
43
| ||
Unknown
|
8
|
36
|
3
|
21
| ||
Follow up (months)
|
Median [range]
|
90 [9-200]
|
97 [50-200]
|
0.687
|
DNA, RNA, and protein extraction
Polymerase Chain Reaction (PCR) and PIK3CA hotspot mutations analysis
Western blot analysis
Immunohistochemistry
Statistical analysis
Results
Absence of activating mutation of the PIK3CA gene in FL
Expression of AKT, activated AKT, and PTEN in FL
Western blot
Immunohistochemistry
Correlations between IHC and WB
Samples | AKT expression | pAKT expression | PTEN expression |
---|---|---|---|
1
|
+
|
-
|
+
|
2
|
+
|
-
|
+
|
3
|
+
|
-
|
+
|
4
|
+
|
+
|
+
|
5
|
+
|
-
|
+
|
6
|
+
|
+
|
+
|
7
|
+
|
+
|
+
|
8
|
+
|
+
|
+
|
9
|
+
|
+
|
+
|
10
|
+
|
-
|
-
|
11
|
+
|
-
|
+
|
12
|
+
|
+
|
+
|
13
|
+
|
+
|
+
|
14
|
+
|
+
|
+
|
15
|
+
|
-
|
+
|
16
|
+
|
-
|
+
|
17
|
+
|
-
|
-
|
18
|
+
|
-
|
+
|
19
|
+
|
-
|
+
|
20
|
+
|
+
|
+
|
21
|
+
|
-
|
+
|
22
|
+
|
-
|
+
|
23
|
+
|
-
|
+
|
24
|
+
|
-
|
+
|
25
|
+
|
-
|
+
|
26
|
+
|
-
|
-
|
27
|
+
|
-
|
+
|
28
|
+
|
-
|
+
|
29
|
+
|
+
|
+
|
30
|
+
|
+
|
-
|
31
|
+
|
+
|
-
|
32
|
+
|
+
|
-
|
33
|
+
|
-
|
-
|
34
|
+
|
-
|
+
|
35
|
+
|
-
|
+
|
36
|
+
|
-
|
-
|
37
|
+
|
-
|
+
|
38
|
+
|
+
|
+
|
39
|
+
|
+
|
+
|
40
|
+
|
+
|
+
|