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Archives of Gynecology and Obstetrics

Erschienen in:

05.05.2023 | Gynecologic Endocrinology and Reproductive Medicine

Construction and evaluation of intrauterine adhesion model in rats by different methods of mechanical injury

verfasst von: Fang Chen, Ying-Xin Gong, Jing-Jing Xiao, Ning-Hong Jiang, Li-Mei Chen, Long Sui

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 1/2023

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Abstract

Purpose

The study aimed to establish a stable and effective animal model for the experimental study of intrauterine adhesion (IUA) by evaluating various mechanical injury methods.

Methods

A total of 140 female rats were divided into four groups according to the extent and area of endometrial injury: group A (excision area: 2.0 × 0.5 cm²), group B (excision area: 2.0 × 0.25 cm²), group C (endometrial curettage) and group D (sham operation). On the 3rd, 7th, 15th and 30th day after the operation, the tissue samples of each group were collected, and the uterine cavity stenosis and histological changes were recorded by HE and Masson staining. Immunohistochemistry of CD31 was applied to visualize microvessel density (MVD). The pregnancy rate and the number of gestational sacs were used to evaluate the reproductive outcome.

Results

The results showed that endometrium injured by small-area endometrial excision or simple curettage could be repaired. The ratio of fibrosis in groups A and B was higher than that in groups C and group D 30 days after modeling (P < 0.001). The number of endometrial glands and MVD in group A was significantly lower than those in groups B, C and D (P < 0.05). The pregnancy rate in group A was 20%, which was lower than that in groups B (33.3%), C (89%) and D (100%) (P < 0.05).

Conclusion

Full-thickness endometrial excision has a high rate of success in constructing stable and effective IUA models in rats.

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Titel: Construction and evaluation of intrauterine adhesion model in rats by different methods of mechanical injury
verfasst von: Fang Chen
Ying-Xin Gong
Jing-Jing Xiao
Ning-Hong Jiang
Li-Mei Chen
Long Sui
Publikationsdatum: 05.05.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 1/2023
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-023-06926-9

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Construction and evaluation of intrauterine adhesion model in rats by different methods of mechanical injury