Correction: Protocatechuic aldehyde acts synergistically with dacarbazine to augment DNA double-strand breaks and promote apoptosis in cutaneous melanoma cells
verfasst von:
Junxia Pei, Zhou Su, Xin Zeng, Ya Zhong, Yamei Zhang, Yixi Yang, Qiuxia Lu, Jian Li, Yu Deng
Correction: BMC Complement Med Ther 23, 111 (2023)
https://doi.org/10.1186/s12906-023-03933-w
Anzeige
Following the publication of the original article [1], it was noted that due to a typesetting error the figure images were paired incorrectly and some information was lost. The correct Figs. 1, 2, 3 and 4 are given below.
Fig. 1
Protocatechuic aldehyde synergistically enhances DTIC cytotoxicity to melanoma cells. A Dose-response curves for PA, DTIC or DTIC combined with a certain concentration of PA for 72 h in A375 and SK-MEL-28 cells. B Dose-response curves for A375 and SK-MEL-28 cells treated with a range of concentrations of PA, DTIC or their combinations. C Synergy scores were calculated from the data represented in B for PA combined with DTIC for A375 and SK-MEL-28 cells. The left panel represents the synergy scores from ZIP model. The right panel represents the synergy scores from Bliss model. **** p<0.0001
Fig. 2
Combination of PA and DTIC increased DNA-double strand breaks and apoptosis in A375 and SK-MEL-28 cells. A The double-strand breaks in A375 and SK-MEL-28 cells were assessed by neutral comet assay. B Quantification of DNA percentages in comet tails in A. C γ-H2AX levels after 72 h of treatment with DMSO, PA, DTIC or combination of PA and DTIC in A375 and SK-MEL-28 cells. D Quantification of the relative γ-H2AX levels in C. E Cell apoptosis was analyzed by flow cytometry. The combined treatment showed the most apoptosis induction. F Quantification of apoptosis ratios in E. G Cleaved caspase-3 protein levels after 72 h of treatment with DMSO, PA, DTIC or combination of PA and DTIC in A375 and SK-MEL-28 cells. Right panel represents quantification of the relative cleaved caspase-3 protein levels in G. ns = no significant,*p < 0.05, **p < 0.01,***p < 0.001 and **** p<0.0001
Fig. 3
PA promotes MGMT degradation in melanoma cells. A-B Left panel shows western blot analysis for MGMT levels in A375 and SK-MEL-28 cells after 72h of treatment with DMSO, PA, DTIC or combination of PA and DTIC. Right panel represents the quantification of the MGMT protein levels for the left panel. C MGMT mRNA expression in A375 and SK-MEL-28 after 72 h PA treatment. D-E A375 and SK-MEL-28 were treated with cycloheximide in the presence or absence PA for 0-8 h hours. Western blot showed the MGMT degradation rates. Right panel represents quantification of the MGMT protein levels. F-G A375 and SK-MEL-28 were treated with MG132 combined with cycloheximide in the presence or absence of PA for 0-8 h hours. The MGMT protein levels were analyzed by western blot. Right panel represents quantification of the MGMT protein levels. ns = no significant, *p < 0.05, **p < 0.01,***p < 0.001 and **** p<0.0001
Fig. 4
MGMT is required for PA-mediated synergistic effect. A The shRNA-mediated knockdown of MGMT was validated by western blot. Right panel represents the quantification of the MGMT protein levels in A. B Knockdown of MGMT increased the DTIC sensitivities in A375 and SK-MEL-28 cells. C Dose-response curves for MGMT-depleted melanoma cells treated with PA, DTIC or their combinations. The selected concentrations for treatment are identical with those in Fig. 1B. D Synergy scores were obtained from the data represented in C. The synergy scores were calculated by ZIP model and Bliss model, respectively. *p < 0.05, **p < 0.01 and **** p < 0.0001
×
×
×
×
The correct figures and captions have been included in this correction, and the original article has been corrected.
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Correction: Protocatechuic aldehyde acts synergistically with dacarbazine to augment DNA double-strand breaks and promote apoptosis in cutaneous melanoma cells
verfasst von
Junxia Pei Zhou Su Xin Zeng Ya Zhong Yamei Zhang Yixi Yang Qiuxia Lu Jian Li Yu Deng
