Background
Methods
Study area and subjects
Polymorphisms in gene | SNP | Method enzimas annealing | Primers | Genotype | Reference |
---|---|---|---|---|---|
IFN γ −183
G > T
|
rs2069709
|
RFLP (53º)
Eco47I
| FW: 5′-AAT GAT CAA TGT GCT TTG TG-3′ R: 5′-TAA GAT GAG ATG GTG ACAG-3′ | TT: 271 pb GT: 271 pb, 164 pb, 107pb GG: 164pb, 107 pb | Suxia Qi et al. [20] |
IFN γ +874
A > T
|
rs2430561
| ASO-PCR (53º) | CP: -5′-TCA ACA AAG CTG ATA CTC CA-3′ T : 5′-TTC TTA CAA CAC AAA ATC AAA TCT-3′ A: 5′-TTC TTA CAA CAC AAA ATC AAA TCA-3′ | AA: 262 pb (reação A) TT: 262 pb (reação T) AT: 262 pb (reação A, T) | Medina et al. [21] |
TNF α −238
G > A
|
rs 361525
| RFLP (60º)
MspI
| FW: 5′-ATC TGG AGG AAG CGG TAG TG-3′ R: 5-AGA AGA CCC CCC TCG GAA CC’ 3′ | AA: 156 pb GG: 133 pb GA: 153 pb, 133 pb | Hedayati et al. [22] |
TNFα −308
G > A
|
rs 1800629
| RFLP (59º)
NcoI
| FW: 5′-GAG GCA ATA GGT TTT GAG GGC CAT-3′ R: 5′-GGG ACA CAC AAG CAT CAAG 3′ | AA: 147 pb GG: 126 pb, 121 pb GA: 147 pb, 126 pb, 21 pb | Asghar et al. [23] |
TNFα −1031
T > C
|
rs1799964
| RFLP (57º)
BbsI
| FW: 5′-TAT GTG ATG GAC TCA CCA GGT-3′ R: 5′-CCT CTA CAT GGC CCT GTC TT 3′ | TT: 251 pb, 13 pb TC: 251 pb, 180 pb, 71 pb e 13 pb CC: 180 pb, 71 pb e 13 pb | Asghar et al. [23] |
IL-10 −592
C > A
|
rs1800872
| RFLP (54º)
RsaI
| FW: 5′-GGG TGA GGA AAC CAA ATT CTC-3′ R:5′-GAG GGG GTG GGC TAA ATA TC 3′ | AA: 240 pb, 77 pb, 36 pb e 08 pb CC: 317 pb,36 pb e 08 pb CA: 317 pb, 240 pb, 77 pb, 36 pb e 08 pb | |
IL-10 −819
C > T
|
rs1800871
| RFLP (54º)
RseI
| FW: 5′-GGG TGA GGA AAC CAA ATT CTC-3′ R: 5′-GAG GGG GTG GGC TAA ATA TC 3′ | TT: 270 pb e 91 pb CC: 217 pb, 91 pb e 53 pb TC: 270 pb, 217 pb, 91 pb e 53 pb |
Assessment of the serological response against Plasmodium vivax
Statistical analysis
Results
Gene | SNP | PvAMA-1 (n = 135) |
P value | PvDBP (n = 135) |
P value | PvMSP-119
(n = 128) |
P value |
---|---|---|---|---|---|---|---|
INFG
| +874T > A
| 0.19 | 0.40 | 0.42 | |||
AA
| 1.36 (0.63–2.78) | 1.58 (0.78–7.29) | 2.92 (1.00–7.63) | ||||
AT
| 1.14 (0.51–2.18) | 1.41 (0.77–4.73) | 2.05 (0.49–6.64) | ||||
TT
| 2.50 (1.18–3.00) | 5.17 (0.86–18.5) | 4.66 (1.50–8.11) | ||||
TNFA
| −1031T > C
| 0.74 | 0.42 | 0.71 | |||
TT
| 1.41 (0.60–2.97) | 1.34 (0.79–5.38) | 3.74 (0.96–7.57) | ||||
TC
| 1.49 (0.58–2.50) | 1.66 (0.79–6.87) | 2.60 (0.51–6.93) | ||||
CC
| 1.45 (1.01–1.52) | 8.57 (0.73–14.89) | 2.12 (1.32–2.59) | ||||
TNFA
| −308G > A
| 0.41 | 0.58 | 0.50 | |||
GG
| 1.67 (0.65–2.80) | 1.66 (0.79–6.30) | 2.92 (0.89–7.58) | ||||
GA
| 1.14 (0.58–2.23) | 1.48 (0.73–3.42) | 2.61 (1.18–6.54) | ||||
AA
| 0.62 (0.51–0.62) | 1.39 (0.96–1.39) | 1.30 (0.28–1.30) | ||||
TNFA
| −238G > A
| 0.76 | 0.97 | 0.61 | |||
GG
| 1.45 (0.64–2.66) | 1.59 (0.81–5.59) | 2.61 (0.94–7.47) | ||||
GA
| 1.34 (0.53–2.91) | 1.48 (0.76–7.16) | 5.15 (1.69–8.16) | ||||
AA
| 2.32 (2.32–2.32) | 1.28 (1.28–1.28) | 3.04 (3.04–3.04) | ||||
IL10
| −819C > T
| 0.79 | 0.57 | 0.99 | |||
CC
| 1.35 (0.63-2.53) | 2.01 (0.79–5.91) | 3.50 (0.55–7.48) | ||||
CT
| 1.45 (0.60-2.77) | 1.29 (0.77–5.06) | 2.61 (0.87–7.60) | ||||
TT
| 2.22 (0.83–2.84) | 2.18 (1.35–6.31) | 1.98 (1.22–7.13) | ||||
IL10
| −592C > A
| 0.86 | 0.86 | 0.77 | |||
CC
| 1.41 (0.62–2.50) | 1.59 (0.76–5.91) | 4.92 (1.10–7.63) | ||||
CA
| 1.52 (0.62–2.78) | 1.46 (0.81–5.46) | 2.59 (0.94–7.26) | ||||
AA
| 1.22 (0.52–2.85) | 1.84 (1.21–4.32) | 2.50 (1.07–5.97) |
Haplotypes | Frequency | PvAMA-1 | PvDBP | PvMSP-119
| ||||||
---|---|---|---|---|---|---|---|---|---|---|
RI estimation | Difference (95 % CI) |
P value | RI estimation | Difference (95 % CI) |
P value | RI estimation | Difference (95 % CI) |
P value | ||
TNF−1031/−308/−238
| ||||||||||
T/G/G | 0.606 | 1.76 | Reference | 4.01 | Reference | 4.35 | Reference | |||
C/G/G | 0.201 | −0.13 (−0.49 to 0.24) | 0.50 | 1.86 (−0.15 to 3.86) | 0.07 | −0.62 (−1.75 to 0.52) | 0.29 | |||
T/A/G | 0.127 | −0.31 (−0.74 to 0.12) | 0.15 | −0.89 (−3.20 to 1.41) | 0.45 | −0.58 (−1.88 to 0.73) | 0.39 | |||
C/G/A | 0.041 | −0.07 (−0.78 to 0.63) | 0.84 | −1.51 −5.17 to 2.15) | 0.42 | 0.19 (−1.99 to 2.36) | 0.86 | |||
IL10−819/−592
| ||||||||||
C/C | 0.644 | 1.54 | Reference | 4.77 | Reference | 4.13 | Reference | |||
T/A | 0.311 | 0.05 (−0.25 to 0.35) | 0.74 | −0.09 (−1.83 to 1.66) | 0.92 | −0.48 (−1.42 to 0.46) | 0.32 | |||
T/C | 0.037 | 0.50 (−0.17 to 1.18) | 0.14 | −1.50 (−5.43 to 2.43) | 0.45 | 1.34 (−0.75 to 3.44) | 0.21 |