Introduction
Vertebral fractures, one of the most typical complications of osteoporosis, are notoriously common among aging populations [
1‐
3]. Vertebral dimensions have been associated with vertebral fracture risk as individuals with small vertebral size seem to be at an elevated risk of sustaining a morphometrically diagnosed vertebral fracture [
4,
5]. While previous research has shown that lifestyle choices in adulthood may influence vertebral size [
6‐
8], the need has arisen to expand current knowledge on the factors affecting one’s vertebral size across the life course.
From the nutritional point of view, calcium (Ca) has attracted the most scientific interest in skeletal research, together with vitamin D [
9,
10]. Achieving a sufficient Ca intake from diet is essential for skeletal health, not only in childhood and adolescence, when peak bone mass is accumulated [
11], but also in later life, when Ca apparently contributes to preventing bone loss and microstructure degradation [
12]. Although several studies have associated Ca intake with enhanced bone geometry at various skeletal sites [
13‐
16], contradicting results have also been found [
17]. Regarding the lumbar spine, we found only one study that addressed the association between Ca intake and vertebral size [
17]: among 111 men aged ≥ 50 years, 2-year consumption of Ca-vitamin-D-fortified milk had no effect on the geometry of L1—L3. Thus, the association between Ca and bone geometry requires further study; studies addressing vertebral geometry should be of particular interest due to the high incidence of vertebral fractures among the elderly [
2,
3].
In the present study, we investigated the relationship between self-reported (dairy- and supplement-based) Ca intake at 31 and 46 years and vertebral cross-sectional area (CSA) at 46 years in a large, representative Finnish birth cohort population. We hypothesized that inadequate Ca intake would be associated with small vertebral CSA in midlife.
Discussion
Using a general population sample of 1064 middle-aged Northern Finns, this prospective study aimed to determine the association of dairy- and supplement-based Ca intake (from age 31 to 46) with vertebral size (age 46). Among women, inadequate Ca intake was associated with 3.8% smaller midlife vertebral CSA than adequate intake over the follow-up. Among men, Ca intake was not associated with vertebral CSA.
Investigations of Ca and bone morphology in the appendicular skeleton have been carried out previously (e.g., reports of larger femoral cross-sectional area [
14‐
16] and enhanced femoral cortical parameters [
13,
17]). However, in these studies, the samples have consisted of mostly women, the results have shown low effect sizes, and the studied doses/cut-offs for Ca intake have varied. Importantly, few reports have addressed the axial skeleton and vertebral morphology. An Australian study [
17] of 111 men aged ≥ 50 years showed that the consumption of Ca-vitamin-D-fortified milk had no effect on vertebral geometry in a 2-year follow-up. In our study, neither the cut-off-based comparisons nor the continuous modeling of Ca intake yielded significant results among men. Despite the different study populations and the various methodological differences between the said study and ours, the present findings regarding men are in line with the previous report. As we observed significant results among women, future studies should confirm this sex discrepancy and investigate the potential underlying factors. However, it should be noted that our sample sizes for men were somewhat lower than those for women, particularly in the inadequate Ca intake group, which may explain the lack of statistical significance among men. Another explanation for the detected sex discrepancy may be the potential pregnancy-associated changes in bone metabolism, although in our previous study [
37], we observed no association between pregnancy, or even multiple pregnancies, and maternal vertebral CSA or shape.
Among women, our models revealed that inadequate Ca intake from age 31 to 46 was associated with smaller vertebral CSA at age 46. As the cut-off for adequate Ca intake was chosen according to the local Finnish [
33] and Nordic [
34] nutrition recommendations, our findings underline the benefits of following these recommendations in terms of gaining and maintaining optimal vertebral CSA. Naturally, several other lifestyle factors are also associated with vertebral size [
6‐
8] and should be accounted for in order to optimize one’s vertebral size. In the time-point-specific models, we observed no association between Ca intake and vertebral CSA, indicating that Ca intake is likely to affect spinal health over a longer period of time.
Generally, small vertebral size increases susceptibility to morphometric vertebral fractures [
4,
5], indicating that studies that aim to reveal lifestyle-related determinants of vertebral size are of high relevance. However, it is not yet known whether this also applies to the more specific, reproducible, and incident fracture predictive morphological fractures that may be without dimensional differences but have cortical disruptions [
38]. The incidence of osteoporotic fractures begins to increase after midlife, especially among women [
39], justifying our investigation of the 46-year population. Although patients with an osteoporotic fracture apparently have inadequate dietary Ca (and vitamin D) intake [
40], recent meta-analyses have questioned the routine use of Ca and vitamin D supplements among older people in terms of reaching higher bone mineral density (BMD) [
41] and lower fracture risk [
42]. The findings of the present study addressed neither vertebral BMD nor fracture history, but are suggestive of decreased spinal resilience among middle-aged women who have not achieved adequate Ca intake in adulthood.
The main strengths of our study were its large general population sample and longitudinal assessment of Ca intake in adulthood. The study population was representative of the general Northern Finnish population [
19], and the 15-year follow-up period, from age 31 to age 46, was rather long. We were also able to control for the potential confounding effect of vitamin D intake on Ca. We obtained data on vertebral geometry from recent MRI scans using a validated approach [
24] with high intra-rater reliability and low measurements errors [
7]. We measured several dimensions to maximize measurement accuracy.
Our study also had limitations. With regard to our Ca variables, the approximation of Ca intake was based on dairy consumption and Ca supplements, and was therefore not fully exhaustive, although dairy products are widely used in the Finnish population [
28]. As other sources yield relatively little of the daily Ca supply among Finns and more precise evaluation of these sources (e.g., the use of plant-based “milks” enriched with Ca, nuts, seeds, soya) was not achievable, we decided to approximate Ca intake as presented. We also used self-reports to gather nutritional data, which is a potential source of bias, although the test-retest reliability of our questionnaire has shown to be high [
43,
44]. For convenience, the questionnaires did not enquire about each food individually but in groups of similar food items, and this may have introduced inaccuracies to our Ca and vitamin D intake estimates. We were unable to take every source of calcium and vitamin D into account when calculating the intake estimates. Instead of self-reported vitamin D intake, the measurement of serum 25-hydroxyvitamin D level would be an interesting target for further studies. The cutoffs for adequate Ca intake were chosen in accordance with local nutrition recommendations and previous literature [
33,
34]. We used the general population reference values, as the study population was representative of the general Finnish population. As our nutritional data from childhood and adolescence were scarce, we were unable to provide Ca intake estimates for these time periods, although they may play an important role in the development of vertebral size. However, according to our recent study, vertebral dimensions and CSA undergo changes in adulthood [
45], suggesting that later life is also relevant in this regard. In addition to Ca, we acknowledge that a wide range of other relevant nutritional factors may affect vertebral size, e.g., protein and fat intake [
10]. Unfortunately, we were unable to provide estimates for protein, fat, or total energy intake due to the structure of the food questionnaires. As for vertebral data, lumbar MRI scans were only obtained in midlife, which prevented us from assessing vertebral size in a longitudinal manner. We also specifically focused on vertebral geometry instead of structural or architectural parameters, which equally influence bone strength [
5] and may be more easily affected by Ca. This choice was made due to the lack of studies using vertebral size as outcome. We measured L4 because it was located in the middle of the lumbar MRI scans, with both axial and sagittal slices available. Vertebral geometry and strength seem to follow a stable pattern across the thoracic and lumbar spine [
27], indicating that our results regarding L4 should be applicable across the thoracolumbar spine. Future studies should aim to compensate the limitations of our data.
We conclude that inadequate Ca intake (< 800 mg/day) in adulthood predicts small vertebral size and thus decreased spinal resilience among middle-aged women. Among men, there was no association between Ca intake and vertebral size. Future studies should confirm these findings and investigate the potential factors underlying the association of low Ca intake in women but not in men with smaller vertebral size. Moreover, although vertebral dimensions have been previously shown to predict morphometric vertebral fractures, it is not yet known whether this also applies to the more specific, reproducible and incident fracture predictive morphological fractures that may be without dimensional differences but have cortical disruptions.
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.