Background
Methods
Patient samples
Bladder cancer cell lines
Extraction of RNA and qRT-PCR
Western blot analysis
Culture of stable cell lines
Cell proliferation
Cell migration and invasion assays
Cellular apoptosis analysis
Immunohistochemistry (IHC) analysis
Statistical analysis
Results
SOX30 expression found to be suppressed in human BC tissue and BC cell lines
Low expression of SOX30 conferred worse patient prognosis in those with BC
Characteristic | case number(n = 56) | SOX30 expression | P-value | |
---|---|---|---|---|
High (n = 35) | Low (n = 21) | |||
Gender | 0.639 | |||
Male | 47 | 30 | 17 | |
Female | 9 | 5 | 4 | |
Age | 0.093 | |||
≥ 60 years | 44 | 30 | 14 | |
<60 years | 12 | 5 | 7 | |
TNM stage | 0.019a | |||
T1-2 | 30 | 23 | 7 | |
T3–4 | 26 | 12 | 14 | |
N status | 0.138 | |||
N0 | 50 | 30 | 20 | |
N1–3 | 6 | 5 | 1 | |
Histologic grade | 0.068 | |||
G1–2 | 19 | 15 | 4 | |
G3–4 | 37 | 20 | 17 | |
Tumor size | 0.150 | |||
≥ 5 cm | 20 | 15 | 5 | |
< 5 cm | 36 | 20 | 16 | |
Pathological type | 0.317 | |||
Urothelium carcinoma | 33 | 18 | 15 | |
Papillary carcinoma | 13 | 11 | 2 | |
Squamous-cell carcinoma | 5 | 4 | 1 | |
Glandular carcinoma | 5 | 2 | 3 |