Background
Methods
Patients and blood samples
Isolation of ZIKV NS1 mAbs from memory B cells
Capture ELISA analysis
Statistics analysis
Results
Expression of recombinant NS1 protein and establishment of capture ELISA
Dynamics and cross-reactivity of NS1 antibody response during natural ZIKV infection
Binding and specificity of NS1 mAbs derived from memory B cells of ZIKV infected patients
mAb ID | Origin | Day | Binding to NS1 (EC50)(ng/mL) | Heavy chain | Light chain | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ZIKV | DENV1 | DENV2 | DENV3 | DENV4 | V family | CDR3 sequence | V gene identity (%) | V family | CDR3 sequence | V gene identity (%) | |||
ZKns3G2 | Pt1 | 188 | 34.5 | N/A | N/A | N/A | N/A | IGHV3–53 | ARERGWLDY | 93.68 | IGKV1–39 | QQTYTIPRT | 92.83 |
ZKns4F10 | Pt1 | 188 | 10.9 | N/A | 116.1 | N/A | 351.4 | IGHV4–31 | ARAIDNFYDNSI | 96.56 | IGKV1–39 | QQSYSPPYT | 94.98 |
ZKns2E11 | Pt2 | 66 | 9.8 | N/A | N/A | N/A | N/A | IGHV3–30-3 | ARVFNGYEGDY | 95.83 | IGLV3–10 | YSTDSSGNLYV | 97.49 |
ZKns14G5 | Pt2 | 66 | 277.4 | N/A | N/A | N/A | N/A | IGHV5–51 | ARSNVDGSTDY | 98.61 | IGLV3–25 | QSADSSDTYVPYV | 98.92 |
ZKns4B8 | Pt2 | 66 | 39.2 | N/A | N/A | N/A | N/A | IGHV3–53 | ASLGSGSAFGY | 96.84 | IGKV1–12 | QQANSFPFT | 98.21 |