Erschienen in:
01.07.2015 | Article
Deletion of the gene for adiponectin accelerates diabetic nephropathy in the Ins2
+/C96Y mouse
verfasst von:
Fei Fang, Eun-Hui Bae, Amanda Hu, George C. Liu, Xiaohua Zhou, Vanessa Williams, Nicholas Maksimowski, Catherine Lu, Ana Konvalinka, Rohan John, James W. Scholey
Erschienen in:
Diabetologia
|
Ausgabe 7/2015
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Abstract
Aims/hypothesis
Diabetic nephropathy is one of the most common forms of chronic kidney disease. The role of adiponectin in the development of diabetic nephropathy has not been elucidated, and the aim of the present study was to investigate the hypothesis that deletion of the gene for adiponectin would accelerate diabetic nephropathy in the Akita mouse.
Methods
We followed four groups of mice from 4 weeks to 16 weeks of age (n ≥ 10 in each group): wild-type (WT) (Ins2
+/+
Adipoq
+/+) mice; APN−/− (Ins2
+/+
Adipoq
−/−) mice; Akita (Ins2
+/C96Y
Adipoq
+/+) mice and Akita/APN−/− (Ins2
+/C96Y
Adipoq
−/−) mice. The mice were then killed and diabetic kidney injury was assessed. In vitro experiments were performed in primary mesangial cells.
Results
Mice from both diabetic groups exhibited increased glomerular adiponectin receptor 1 (adipoR1) expression, kidney hypertrophy, glomerular enlargement, increased albuminuria and tissue oxidative stress compared with the WT control. Deletion of the adiponectin gene had no effect on glycaemia. However, Akita/APN−/− mice exhibited a greater extent of renal hypertrophy. In vitro, adiponectin attenuated high-glucose-induced phosphorylation of mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase (S6K). A higher level of fibrosis was observed in the tubulointerstitial and glomerular compartments of the Akita/APN−/− mice and adiponectin was found to inhibit TGFβ-induced Smad2 and Smad3 phosphorylation in vitro. There was an exaggerated inflammatory response in the Akita/APN−/− mice. Adiponectin also inhibited high-glucose-induced activation of nuclear factor κB (NFκB) in mesangial cells.
Conclusions/interpretation
Our data suggest that adiponectin is an important determinant of the kidney response to high glucose in vivo and in vitro.