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Erschienen in: Archives of Virology 11/2015

01.11.2015 | Review

Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China

verfasst von: Jun Zhou, Xudong Fan, Yafeng Dong, Zun ping Zhang, Fang Ren, Guojun Hu

Erschienen in: Archives of Virology | Ausgabe 11/2015

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Abstract

To investigate the prevalence and genetic variation of grapevine fanleaf virus (GFLV) in China, 142 grapevine samples from 13 provinces and regions were tested using DAS-ELISA, RT-PCR, and nested RT-PCR. Of the samples, 38 % tested positive for GFLV by DAS-ELISA, and 26.8 % tested positive by RT-PCR and nested RT-PCR. Movement protein (MP) and coat protein (CP) gene PCR products were cloned and sequenced. The MP or CP nucleotide and protein sequences shared identities that ranged from 94.9 % to 100 %. Phylogenetic analysis revealed that Chinese GFLV isolates obtained in this study were distinct from the isolates reported in GenBank.
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Literatur
1.
Zurück zum Zitat Andret-Link P, Laporte C, Valat L (2004) Grapevine fanleaf virus: still a major threat to the grapevine industry. J Plant Pathol 86(3):183–195 Andret-Link P, Laporte C, Valat L (2004) Grapevine fanleaf virus: still a major threat to the grapevine industry. J Plant Pathol 86(3):183–195
2.
Zurück zum Zitat Bashir NS, Hajizadeh M (2007) Survey for Grapevine fanleaf virus in vineyards of north-west Iran and genetic diversity of isolates. Australas Plant Pathol 36:46–52CrossRef Bashir NS, Hajizadeh M (2007) Survey for Grapevine fanleaf virus in vineyards of north-west Iran and genetic diversity of isolates. Australas Plant Pathol 36:46–52CrossRef
3.
Zurück zum Zitat Bashir NS, Melcher U (2012) Population genetic analysis of Grapevine fanleaf virus. Arch Virol 157:1919–1929CrossRef Bashir NS, Melcher U (2012) Population genetic analysis of Grapevine fanleaf virus. Arch Virol 157:1919–1929CrossRef
4.
Zurück zum Zitat Bertolini E, García J, Yuste A, Olmos A (2010) High prevalence of viruses in table grape from Spain detected by real-time RT-PCR. Eur J Plant Pathol 128:283–287CrossRef Bertolini E, García J, Yuste A, Olmos A (2010) High prevalence of viruses in table grape from Spain detected by real-time RT-PCR. Eur J Plant Pathol 128:283–287CrossRef
5.
Zurück zum Zitat Boulila M (2007) Phylogeny and genetic recombination of Grapevine fanleaf virus isolates from naturally infected vineyards in Tunisia. Phytopathol Mediterr 46:285–294 Boulila M (2007) Phylogeny and genetic recombination of Grapevine fanleaf virus isolates from naturally infected vineyards in Tunisia. Phytopathol Mediterr 46:285–294
6.
Zurück zum Zitat Chen S, Zhou Y, Ye T, Hao L, Guo L, Fan Z, Li S, Zhou T (2014) Genetic variation analysis of Apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China. Arch Virol 159(6):1431–1438CrossRefPubMed Chen S, Zhou Y, Ye T, Hao L, Guo L, Fan Z, Li S, Zhou T (2014) Genetic variation analysis of Apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China. Arch Virol 159(6):1431–1438CrossRefPubMed
7.
Zurück zum Zitat Chung CT, Niemela SA, Miller RH (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175PubMedCentralCrossRefPubMed Chung CT, Niemela SA, Miller RH (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175PubMedCentralCrossRefPubMed
8.
Zurück zum Zitat Fiore N, Zamorano A, Rivera L, Gonzalez F, Aballay E, Montealegre J, Pino AM (2011) Grapevine viruses in the Atacama region of Chile. J Phytopathol 159:743–750CrossRef Fiore N, Zamorano A, Rivera L, Gonzalez F, Aballay E, Montealegre J, Pino AM (2011) Grapevine viruses in the Atacama region of Chile. J Phytopathol 159:743–750CrossRef
9.
Zurück zum Zitat Gambino G, Bondaz J, Gribaudo I (2006) Detection and elimination of viruses in callus, somatic embryos and regenerated plantlets of grapevine. Eur J Plant Pathol 114:397–404CrossRef Gambino G, Bondaz J, Gribaudo I (2006) Detection and elimination of viruses in callus, somatic embryos and regenerated plantlets of grapevine. Eur J Plant Pathol 114:397–404CrossRef
10.
Zurück zum Zitat Ge B, He Z, Zhang Z, Wang H, Li S (2014) Genetic variation in potato virus M isolates infecting pepino (Solanum muricatum) in China. Arch Virol 159(12):3197–3210CrossRefPubMed Ge B, He Z, Zhang Z, Wang H, Li S (2014) Genetic variation in potato virus M isolates infecting pepino (Solanum muricatum) in China. Arch Virol 159(12):3197–3210CrossRefPubMed
12.
Zurück zum Zitat Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452CrossRefPubMed Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452CrossRefPubMed
13.
Zurück zum Zitat Liebenberg A, Freeborough MJ, Visser CJ, Bellstedt DU, Burger JT (2009) Genetic variability within the coat protein gene of Grapevine fanleaf virus isolates from South Africa and the evaluation of RT-PCR, DAS-ELISA and immunostrips as virus diagnostic assays. Virus Res 142:28–35CrossRefPubMed Liebenberg A, Freeborough MJ, Visser CJ, Bellstedt DU, Burger JT (2009) Genetic variability within the coat protein gene of Grapevine fanleaf virus isolates from South Africa and the evaluation of RT-PCR, DAS-ELISA and immunostrips as virus diagnostic assays. Virus Res 142:28–35CrossRefPubMed
14.
Zurück zum Zitat Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analysing recombination. Bioinformatics 26:2462–2463PubMedCentralCrossRefPubMed Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analysing recombination. Bioinformatics 26:2462–2463PubMedCentralCrossRefPubMed
15.
Zurück zum Zitat MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226CrossRef MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226CrossRef
16.
Zurück zum Zitat Mahfoudhi N, Harbi-Ben SM, Elair M, Selmi I, Ben Hamda H (2014) Prevalence of viruses infecting autochthonous grapevines in Tunisia. Tunisian J Plant Prot 9:111–118 Mahfoudhi N, Harbi-Ben SM, Elair M, Selmi I, Ben Hamda H (2014) Prevalence of viruses infecting autochthonous grapevines in Tunisia. Tunisian J Plant Prot 9:111–118
17.
Zurück zum Zitat Maliogka V, Dovas CI, Efthimiou K, Katis NI (2004) Detection and differentiation of Comoviridae species using a semi-nested RT-PCR and a phylogenetic analysis based on the polymerase protein. J Phytopathol 152:404–409CrossRef Maliogka V, Dovas CI, Efthimiou K, Katis NI (2004) Detection and differentiation of Comoviridae species using a semi-nested RT-PCR and a phylogenetic analysis based on the polymerase protein. J Phytopathol 152:404–409CrossRef
18.
Zurück zum Zitat Mekuria T, Martin RR, Naidu RA (2008) The occurrence of Grapevine fanleaf Virus in Washington State Vineyards. In: Proceedings of the 2nd annual national viticulture research conference, July 9–11, University of California, Davis Mekuria T, Martin RR, Naidu RA (2008) The occurrence of Grapevine fanleaf Virus in Washington State Vineyards. In: Proceedings of the 2nd annual national viticulture research conference, July 9–11, University of California, Davis
19.
Zurück zum Zitat Myles S, Boykob AR, Owens CL, Brown PJ, Grassi F, Aradhya MK, Prins B, Reynolds A, Jer-Ming Chia, Ware D, Carlos D, Bustamante, Buckler ES (2011) Genetic structure and domestication history of the grape. Proc Natl Acad Sci USA 108:3530–3535PubMedCentralCrossRefPubMed Myles S, Boykob AR, Owens CL, Brown PJ, Grassi F, Aradhya MK, Prins B, Reynolds A, Jer-Ming Chia, Ware D, Carlos D, Bustamante, Buckler ES (2011) Genetic structure and domestication history of the grape. Proc Natl Acad Sci USA 108:3530–3535PubMedCentralCrossRefPubMed
20.
Zurück zum Zitat Oliver JE, Vigne E, Fuchs M (2010) Genetic structure and molecular variability of Grapevine fanleaf virus populations. Virus Res 152:30–40CrossRefPubMed Oliver JE, Vigne E, Fuchs M (2010) Genetic structure and molecular variability of Grapevine fanleaf virus populations. Virus Res 152:30–40CrossRefPubMed
21.
Zurück zum Zitat Poljua D, Sladonja B, Bubola M (2010) Incidence of viruses infecting grapevine varieties in Istria (Croatia). J Food Agric Environ 8:166–169 Poljua D, Sladonja B, Bubola M (2010) Incidence of viruses infecting grapevine varieties in Istria (Croatia). J Food Agric Environ 8:166–169
22.
Zurück zum Zitat Pompe-Novak M, Gutierrez-Aguirre I, Vojvoda J, Blas M, Tomazic I, Vigne E, Fuchs M, Ravnikar M, Petrovic N (2007) Genetic variability within RNA2 of Grapevine fanleaf virus. Eur J Plant Pathol 117:307–312CrossRef Pompe-Novak M, Gutierrez-Aguirre I, Vojvoda J, Blas M, Tomazic I, Vigne E, Fuchs M, Ravnikar M, Petrovic N (2007) Genetic variability within RNA2 of Grapevine fanleaf virus. Eur J Plant Pathol 117:307–312CrossRef
23.
Zurück zum Zitat Reynard JS, Gugerli P (2012) Current status of major grapevine viruses in La Côte vineyards of Switzerland. In: 17th ICVG Congress, Davis (US), pp 7–14 Reynard JS, Gugerli P (2012) Current status of major grapevine viruses in La Côte vineyards of Switzerland. In: 17th ICVG Congress, Davis (US), pp 7–14
24.
Zurück zum Zitat Rowhani A, Walker MA, Rokni S (1992) Sampling strategies for the detection of Grapevine fanleaf virus and the grapevine strain of Tomato ringspot virus. Vitis 31(1):35–44 Rowhani A, Walker MA, Rokni S (1992) Sampling strategies for the detection of Grapevine fanleaf virus and the grapevine strain of Tomato ringspot virus. Vitis 31(1):35–44
25.
Zurück zum Zitat Sanfacon H, Wellink J, Le Gall O, Karasev A, van der Vlugt R, Wetzel T (2009) Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Arch Virol 154:899–907CrossRefPubMed Sanfacon H, Wellink J, Le Gall O, Karasev A, van der Vlugt R, Wetzel T (2009) Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Arch Virol 154:899–907CrossRefPubMed
26.
Zurück zum Zitat Sanfacon H, Gorbalenya AR, Knowles NJ, Chen JP (2012) Order Picornavirales. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Classification and nomenclature of viruses. Ninth report of the international committee on taxonomy of viruses. Elsevier Inc., Amsterdam, pp 835–839 Sanfacon H, Gorbalenya AR, Knowles NJ, Chen JP (2012) Order Picornavirales. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Classification and nomenclature of viruses. Ninth report of the international committee on taxonomy of viruses. Elsevier Inc., Amsterdam, pp 835–839
27.
Zurück zum Zitat Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30(12):2725–2729PubMedCentralCrossRefPubMed Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30(12):2725–2729PubMedCentralCrossRefPubMed
28.
Zurück zum Zitat Terlizzi F, Pisi A, Beber R, Fiore N, Zamorano A, Credi R, Ratti C (2015) Genetic variabitily of the movement and coat protein genes of Grapevine fanleaf virus isolates from Italy. J Gen Plant Pathol 81:63–67CrossRef Terlizzi F, Pisi A, Beber R, Fiore N, Zamorano A, Credi R, Ratti C (2015) Genetic variabitily of the movement and coat protein genes of Grapevine fanleaf virus isolates from Italy. J Gen Plant Pathol 81:63–67CrossRef
29.
Zurück zum Zitat Wen WG, Cui JX, Sheng L (2007) Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR. ACTA Phytophylacica Sinica 34(1):61–65 Wen WG, Cui JX, Sheng L (2007) Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR. ACTA Phytophylacica Sinica 34(1):61–65
Metadaten
Titel
Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China
verfasst von
Jun Zhou
Xudong Fan
Yafeng Dong
Zun ping Zhang
Fang Ren
Guojun Hu
Publikationsdatum
01.11.2015
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 11/2015
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2566-0

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