Background
Methods
Setting, study population and enrolment
Measures and procedures
Development of IgM MAC-ELISA assay
Determination of MAC-ELISA optical density (OD) cut-off
Assessment for MAC-ELISA assay cross-reactivity with other pathogens
Sample testing by IgM MAC-ELISA
Sample testing by MAT
Validation of IgM MAC-ELISA
Results
n
| % | |
---|---|---|
Total Participants | 63 | 100 |
Sex | ||
Male | 42 | 66.7 |
Female | 21 | 33.3 |
Location (district, towna) | ||
Junin | 3 | 4.8 |
Madre de Dios | 1 | 1.6 |
Tumbes (Tumbes) | 4 | 6.3 |
Loreto (Yurimaguas) | 9 | 14.3 |
Loreto (Iquitos) | 46 | 73 |
Age (years) | ||
Median | 22 | |
Range | 9 - 58 |
Sensitivity% | Specificity% | PPV% | NPV% | |
---|---|---|---|---|
(CI 95%) | (CI 95%) | (CI 95%) | (CI 95%) | |
ELISA IgM, | 92.3 | 56.0 | 35.3 | 96.6 |
Acute | (85.7 - 98.9) | (43.7 - 68.3) | (23.5 - 47.1) | (92.05 - 100) |
ELISA IgM, | 93.3 | 51.5 | 63.6 | 89.5 |
Convalescent | (87.2 - 99.5) | (39.2 - 63.9) | (51.8 - 75.5) | (81.9 - 97.1) |
ELISA IgM, | 93.6 | 37.5 | 59.2 | 85.7 |
Paired | (87.5 - 99.6) | (25.5 - 49.5) | (47.1 - 71.3) | (77.1 - 94.4) |