Erschienen in:
01.04.2016
Detection of virus in CSF from the cases with meningoencephalitis by next-generation sequencing
verfasst von:
Hongzhi Guan, Ao Shen, Xia Lv, Xunzhe Yang, Haitao Ren, Yanhuan Zhao, Yinxin Zhang, Yanping Gong, Peixiang Ni, Honglong Wu, Yicheng Zhu, Liying Cui
Erschienen in:
Journal of NeuroVirology
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Ausgabe 2/2016
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Abstract
We screened for viral DNA in cerebrospinal fluid samples using next-generation sequencing (NGS) technology to diagnose CNS viral infections. We collected CSF samples from four cases with clinically suspected viral meningoencephalitis. DNA extracted from the samples was analyzed with NGS, and the results were further validated using PCR. Herpes simplex virus 1 (HSV-1) was detected in the CSF of two patients, HSV-2 and human herpes virus type 3 (HHV-3, VZV) in the CSF of two other patients separately. The number of unique reads of the identified viral genes ranged from 144 to 44205 (93.51 to 99.57 %). The coverage of identified viral genes ranged from 12 to 98 % with a depth value of 1.1 to 35, respectively. The results were further confirmed using PCR in three cases. The clinical presentation and outcomes of these four cases were consistent with the diagnostic results of NGS. NGS of CSF samples can be used as a diagnostic assay for CNS viral infection. Its further application for “pan-viral” or even “pan-microbial” screening of CSF might influence the diagnosis of CNS infectious diseases.