Erschienen in:
01.03.2020 | Brief Report
Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen
verfasst von:
Qi Zhou, Zhuangli Bi, Dongdong Yin, Xiangxue Gu, Zejun Xu, Rong Huang, Xue Xing, Kezong Qi, Guijun Wang
Erschienen in:
Archives of Virology
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Ausgabe 3/2020
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Abstract
Duck Tembusu virus (DTMUV) has caused significant economic losses in China since 2010. However, there is still a lack of effective methods to diagnose the disease caused by this virus, and especially to differentiate infection from vaccination. In this study, we established a novel indirect enzyme-linked immunosorbent assay (iELISA) and performed a retrospective serological survey for DTMUV in Anhui province, China. Our results show that the iELISA displayed high specificity sensitivity, and with no serological cross-reaction with other duck pathogens. These findings indicate that the newly developed iELISA could be a useful screening tool for large-scale monitoring of the epidemiology of DTMUV infection in ducks.