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Journal of Assisted Reproduction and Genetics

01.01.2008 | Animal

Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 1/2008

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Abstract

Purpose

The aim of this study was to investigate the developmental potential of isolated blastomeres in the presence and absence of leukemia inhibitory factor (LIF) and granulocyte–macrophage colony stimulating factor (GM-CSF).

Methods

The blastomeres of two (1/2) and eight cells (1/8) embryos were isolated and cultured in T6 medium in the presence and absence of LIF (1,000 IU/ml) and or GM-CSF (2 ng/ml) up to 120 h. The diameter and cell number of blastocysts were measured.

Results

The developmental rates of 1/2 isolated blastomeres developed to blastocysts stages in the presence and absence of LIF and GM-CSF were 45.80, 35.10 and 48.66, 41.66, respectively. The diameter of blastocysts was higher in GM-CSF group and total cell number of blastocyst in both treated groups was higher than control (P < 0.05). No 1/8 blastomeres developed to morula and blastocyst stages.

Conclusions

LIF and GM-CSF could improve the development of 1/2 isolated blastomeres.
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Metadaten
Titel
Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF
Publikationsdatum
01.01.2008
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 1/2008
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-007-9191-0

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