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Journal of Cancer Research and Clinical Oncology

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01.10.2004 | Original Paper

Differential proteomic analysis of human hepatocellular carcinoma cell line metastasis-associated proteins

verfasst von: Jie-Feng Cui, Yin-Kun Liu, Bo-Shen Pan, Hai-Yan Song, Yu Zhang, Rui-Xia Sun, Jie Chen, Ju-Tao Feng, Zhao-You Tang, Yan-Ling Yu, Hua-Li Shen, Peng-Yuan Yang

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 10/2004

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Abstract

Purpose

The comparative study of differentially expression of protein profiles of hepatocellular carcinoma cell lines with various metastasic potential and screening key molecules related to hepatocellular carcinoma metastasis and recurrence.

Methods

Using two-dimensional electrophoresis and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), we analyzed differentially displayed proteomics of human hepatocellular carcinoma cell lines Hep3B, MHCC97L, MHCC97H with different metastasic potential.

Results

Approximate 1,000 protein spots were detected on silver-stained gel by ImageMaster (977±113 spots in Hep3B, 1092±40 in MHCC97L, and 889±14 in MHCC97H). Fifty distinct different protein spots were analyzed with online LC-ESI-MS/MS. Only 26 protein spots had a positive result, including annexin1, S100A4, and so on. In comparison with nonmetastasis Hep3B cell lines, there were 16 proteins overexpressed in MHCC97H and MHCC97L, 10 proteins underexpressed in MHCC97H and MHCC97L. Applying cell immunohistochemistry and RT-PCR, we further validated two interesting and different proteins, annexin1 and S100A4.

Conclusion

The protein profile of metastatic hepatocellular carcinoma cell lines displayed obvious differences compared with non-metastatic liver cancer cell lines. The results imply that various different proteins may lead to HCC metastasis together.

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Titel: Differential proteomic analysis of human hepatocellular carcinoma cell line metastasis-associated proteins
verfasst von: Jie-Feng Cui
Yin-Kun Liu
Bo-Shen Pan
Hai-Yan Song
Yu Zhang
Rui-Xia Sun
Jie Chen
Ju-Tao Feng
Zhao-You Tang
Yan-Ling Yu
Hua-Li Shen
Peng-Yuan Yang
Publikationsdatum: 01.10.2004
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 10/2004
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-004-0576-5

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Springer Medizin

Differential proteomic analysis of human hepatocellular carcinoma cell line metastasis-associated proteins

Abstract

Purpose

Methods

Results

Conclusion

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

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