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Drug Safety
Erschienen in: Drug Safety 7/2015

01.07.2015 | Leading Article

DNA Barcoding and Pharmacovigilance of Herbal Medicines

verfasst von: Hugo J. de Boer, Mihael C. Ichim, Steven G. Newmaster

Erschienen in: Drug Safety | Ausgabe 7/2015

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Abstract

Pharmacovigilance of herbal medicines relies on the product label information regarding the ingredients and the adherence to good manufacturing practices along the commercialisation chain. Several studies have shown that substitution of plant species occurs in herbal medicines, and this in turn poses a challenge to herbal pharmacovigilance as adverse reactions might be due to adulterated or added ingredients. Authentication of constituents in herbal medicines using analytical chemistry methods can help detect contaminants and toxins, but are often limited or incapable of detecting the source of the contamination. Recent developments in molecular plant identification using DNA sequence data enable accurate identification of plant species from herbal medicines using defined DNA markers. Identification of multiple constituent species from compound herbal medicines using amplicon metabarcoding enables verification of labelled ingredients and detection of substituted, adulterated and added species. DNA barcoding is proving to be a powerful method to assess species composition in herbal medicines and has the potential to be used as a standard method in herbal pharmacovigilance research of adverse reactions to specific products.
Literatur
1.
Mann RD, Andrews EB. Pharmacovigilance. Chichester: Wiley; 2007.
2.
World Health Organization. The importance of pharmacovigilance. Geneva: World Health Organization; 2002.
3.
Meyboom RH, Egberts AC, Edwards IR, Hekster YA, de Koning FH, Gribnau FW. Principles of signal detection in pharmacovigilance. Drug Saf. 1997;16:355–65.PubMedCrossRef
4.
Bergvall T, Norén GN, Lindquist M. vigiGrade: a tool to identify well-documented individual case reports and highlight systematic data quality issues. Drug Saf. 2014;37:65–77.PubMedCrossRef
5.
6.
7.
8.
Applequist WL, Miller JS. Selection and authentication of botanical materials for the development of analytical methods. Anal Bioanal Chem. 2013;405:4419–28.PubMedCrossRef
9.
Pan S, Neeraj A, Srivastava KS, Kishore P, Danquah MK, Sarethy IP. A proposal for a quality system for herbal products. J Pharm Sci. 2013;102:4230–41.PubMedCrossRef
10.
11.
Wolfender J-L. HPLC in natural product analysis: the detection issue. Planta Med. 2009;75:719–34.PubMedCrossRef
12.
Li XQ, Sun XH, Cai S, Ying XX, Li FM. Investigation on the chemical constituents and variation of the flower buds of Lonicera species by UPLC-ESI-MS/MS and principle component analysis. Acta Pharm Sin. 2009;44:895–904.
13.
Zhang C, Su J. Application of near infrared spectroscopy to the analysis and fast quality assessment of traditional Chinese medicinal products. Acta Pharm Sin B. 2014;4:182–92.CrossRef
14.
Jiang Y, David B, Tu P, Barbin Y. Recent analytical approaches in quality control of traditional Chinese medicines—a review. Anal Chim Acta. 2010;657:9–18.PubMedCrossRef
15.
Drašar P, Moravcova J. Recent advances in analysis of Chinese medical plants and traditional medicines. J Chromatogr B. 2004;812:3–21.CrossRef
16.
Scora RW. Problems in chemotaxonomy: the influence of varying soil conditions, of geographical and individual variants upon the distribution of certain substances in chromatographed extracts of Monarda fistulosa. Plant Soil. 1966;24:145–52.CrossRef
17.
Zewdie Y, Bosland PW. Capsaicinoid profiles are not good chemotaxonomic indicators for Capsicum species. Biochem Syst Ecol. 2001;29:161–9.PubMedCrossRef
18.
19.
Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH. Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci USA. 2005;102:8369–74.PubMedCentralPubMedCrossRef
20.
Jarman SN, Elliott NG. DNA evidence for morphological and cryptic Cenozoic speciations in the Anaspididae’, living fossils’ from the Triassic. J Evol Biol. 2000;13:624–33.CrossRef
21.
Burns JM, Janzen DH, Hajibabaei M, Hallwachs W, Hebert PDN. DNA barcodes and cryptic species of skipper butterflies in the genus Perichares in Area de Conservacion Guanacaste, Costa Rica. Proc Natl Acad Sci USA. 2008;105:6350–5.PubMedCentralPubMedCrossRef
22.
Ragupathy S, Newmaster SG, Murugesan M, Balasubramaniam V. DNA barcoding discriminates a new cryptic grass species revealed in an ethnobotany study by the hill tribes of the Western Ghats in southern India. Mol Ecol Resour. 2009;9:164–71.PubMedCrossRef
23.
Liu JIE, Moeller M, Gao L-M, Zhang D-Q, Li D-Z. DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species. Mol Ecol Resour. 2011;11:89–100.
24.
De Carvalho MR, Bockmann FA, Amorim DS, Brandão CRF, de Vivo M, de Figueiredo JL, et al. Taxonomic impediment or impediment to taxonomy? A commentary on systematics and the cybertaxonomic-automation paradigm. Evol Biol. 2007;34:140–3.CrossRef
25.
26.
Boessenkool S, McGlynn G, Epp LS, Taylor D, Pimentel M, Gizaw A, et al. Use of ancient sedimentary DNA as a novel conservation tool for high-altitude tropical biodiversity. Conserv Biol. 2014;28:446–55.PubMedCrossRef
27.
Parducci L, Jørgensen T, Tollefsrud MM, Elverland E, Alm T, Fontana SL, et al. Glacial survival of boreal trees in Northern Scandinavia. Science. 2012;335:1083–6.PubMedCrossRef
28.
Willerslev E, Davison J, Moora M, Zobel M, Coissac E, Edwards ME, et al. Fifty thousand years of Arctic vegetation and megafaunal diet. Nature. 2014;506:47–51.PubMedCrossRef
29.
Van Geel B, Aptroot A, Baittinger C, Birks HH, Bull ID, Cross HB, et al. The ecological implications of a Yakutian mammoth’s last meal. Q Res. 2008;69:361–76.CrossRef
30.
Coghlan M, Haile J, Houston J, Murray D, White N, Moolhuijzen P, et al. Deep sequencing of plant and animal DNA contained within traditional chinese medicines reveals legality issues and health safety concerns. PLoS Genet. 2012;8:e1002657.PubMedCentralPubMedCrossRef
31.
Newmaster SG, Grguric M, Shanmughanandhan D, Ramalingam S, Ragupathy S. DNA barcoding detects contamination and substitution in North American herbal products. BMC Med. 2013;11:222.PubMedCentralPubMedCrossRef
32.
Kerr KCR, Stoeckle MY, Dove CJ, Weigt LA, Francis CM, Hebert PDN. Comprehensive DNA barcode coverage of North American birds. Mol Ecol Notes. 2007;7:535–43.PubMedCentralPubMedCrossRef
33.
Smith MA, Poyarkov NA, Hebert PDN. CO1 DNA barcoding amphibians: take the chance, meet the challenge. Mol Ecol Resour. 2008;8:235–46.PubMedCrossRef
34.
Eurlings MCM, Heuveling van Beek H, Gravendeel B. Polymorphic microsatellites for forensic identification of agarwood (Aquilaria crassna). Forensic Sci Int. 2010;197:30–4.PubMedCrossRef
35.
Chen S, Yao H, Han J, Liu C, Song J, Shi L, et al. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One. 2010;5:1–8.
36.
Li DZ, Gao LM, Li HT, Wang H, Ge XJ, Liu JQ, et al. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. Proc Natl Acad Sci USA. 2011;108:19641–6.PubMedCentralPubMedCrossRef
37.
Kool A, de Boer HJ, Krüger Å, Rydberg A, Abbad A, Björk L, et al. Molecular identification of commercialized medicinal plants in Southern Morocco. PLoS One. 2012;7:e39459.PubMedCentralPubMedCrossRef
38.
Thompson KA, Newmaster SG. Molecular taxonomic tools provide more accurate estimates of species richness at less cost than traditional morphology-based taxonomic practices in a vegetation survey. Biodivers Conserv. 2014;23:1411–24.CrossRef
39.
Cho Y, Mower JP, Qiu Y, Palmer JD. Mitochondrial substitution rates are extraordinarily elevated and variable in a genus of flowering plants. Proc Natl Acad Sci USA. 2004;101:17741–6.PubMedCentralPubMedCrossRef
40.
Kress WJ, Erickson DL. A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS One. 2007;2:e508.PubMedCentralPubMedCrossRef
41.
Fazekas AJ, Burgess KS, Kesanakurti PR, Graham SW, Newmaster SG, Husband BC, et al. Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. PLoS One. 2008;3:e2802.PubMedCentralPubMedCrossRef
42.
Fazekas AJ, Kesanakurti PR, Burgess KS, Percy DM, Graham SW, Barrett SCH, et al. Are plant species inherently harder to discriminate than animal species using DNA barcoding markers? Mol Ecol Resour. 2009;9:130–9.PubMedCrossRef
43.
Ford CS, Ayres KL, Toomey N, Haider N, van Alphen Stahl J, Kelly LJ, et al. Selection of candidate coding DNA barcoding regions for use on land plants. Bot J Linn Soc. 2009;159:1–11.CrossRef
44.
45.
46.
Burgess KS, Fazekas AJ, Kesanakurti PR, Graham SW, Husband BC, Newmaster SG, et al. Discriminating plant species in a local temperate flora using the rbcL + matK DNA barcode. Methods Ecol Evol. 2011;2:333–40.CrossRef
47.
Paton AJ, Brummitt N, Govaerts R, Harman K, Hinchcliffe S, Allkin B, et al. Towards Target 1 of the Global Strategy for Plant Conservation: a working list of all known plant species—progress and prospects. Taxon. 2008;57:602–11.
48.
49.
Newmaster SG, Fazekas AJ, Ragupathy S. DNA barcoding in land plants: evaluation of rbcL in a multigene tiered approach. Botany. 2006;84:335–41.
50.
De Vere N, Rich TC, Ford CR, Trinder SA, Long C, Moore CW, et al. DNA barcoding the native flowering plants and conifers of Wales. PLoS One. 2012;7:e37945.PubMedCentralPubMedCrossRef
51.
Kuzmina ML, Johnson KL, Barron HR, Hebert PD. Identification of the vascular plants of Churchill, Manitoba, using a DNA barcode library. BMC Ecol. 2012;12:25.PubMedCentralPubMedCrossRef
52.
Liu J, Yan H-F, Newmaster SG, Pei N, Ragupathy S, Ge X-J. The use of DNA barcoding as a tool for the conservation biogeography of subtropical forests in China. Divers Distrib. 2015;21:188–99.CrossRef
53.
Ebihara A, Nitta JH, Ito M. Molecular species identification with rich floristic sampling: DNA barcoding the pteridophyte flora of Japan. PLoS One. 2010;5:e15136.PubMedCentralPubMedCrossRef
54.
55.
Soltis PS, Soltis DE. The role of hybridization in plant speciation. Annu Rev Plant Biol. 2009;60:561–88.PubMedCrossRef
56.
Mayrose I, Zhan SH, Rothfels CJ, Magnuson-Ford K, Barker MS, Rieseberg LH, et al. Recently formed polyploid plants diversify at lower rates. Science. 2011;333:1257.PubMedCrossRef
57.
Wood TE, Takebayashi N, Barker MS, Mayrose I, Greenspoon PB, Rieseberg LH. The frequency of polyploid speciation in vascular plants. Proc Natl Acad Sci USA. 2009;106:13875–9.PubMedCentralPubMedCrossRef
58.
Knowles LL, Carstens BC. Delimiting species without monophyletic gene trees. Syst Biol. 2007;56:887–95.PubMedCrossRef
59.
De Boer HJ, Ouarghidi A, Martin G, Abbad A, Kool A. DNA barcoding reveals limited accuracy of identifications based on folk taxonomy. PLoS One. 2014;9:e84291.PubMedCentralPubMedCrossRef
60.
61.
Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, et al. GenBank. Nucleic Acids Res. 2013;41(Database issue):D36–42.
62.
63.
64.
65.
Lou S-K, Wong K-L, Li M, But PP, Tsui SK, Shaw P-C. An integrated web medicinal materials DNA database: MMDBD (Medicinal Materials DNA Barcode Database). BMC Genom. 2010;11:402.CrossRef
66.
Heubl G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. Planta Med. 2010;76:1963–74.PubMedCrossRef
67.
Techen N, Parveen I, Pan Z, Khan IA. DNA barcoding of medicinal plant material for identification. Curr Opin Biotechnol. 2014;25:103–10.PubMedCrossRef
68.
Purushothaman N, Newmaster SG, Ragupathy S, Stalin N, Suresh D, Arunraj DR, et al. A tiered barcode authentication tool to differentiate medicinal Cassia species in India. Genet Mol Res. 2014;13:2959–68.PubMedCrossRef
69.
Little DP, Gulick P. Authentication of Ginkgo biloba herbal dietary supplements using DNA barcoding. Genome. 2014;57:1–4.CrossRef
70.
Kazi T, Hussain N, Bremner P, Slater A, Howard C. The application of a DNA-based identification technique to over-the-counter herbal medicines. Fitoterapia. 2013;87:27–30.PubMedCrossRef
71.
Wallace LJ, Boilard SM, Eagle SH, Spall JL, Shokralla S, Hajibabaei M. DNA barcodes for everyday life: routine authentication of natural health products. Food Res Int. 2012;49:446–52.CrossRef
72.
Zuo Y, Chen Z, Kondo K, Funamoto T, Wen J, Zhou S. DNA barcoding of Panax species. Planta Med. 2011;77:182–7.PubMedCrossRef
73.
Vassou SL, Kusuma G, Madasamy P. DNA barcoding for species identification from dried and powdered plant parts: a case study with authentication of the raw drug market samples of Sida cordifolia. Gene. 2015;559(1):86–93.PubMedCrossRef
74.
Seethapathy GS, Ganesh D, Kumar JUS, Senthilkumar U, Newmaster SG, Ragupathy S, et al. Assessing product adulteration in natural health products for laxative yielding plants, Cassia, Senna, and Chamaecrista, in Southern India using DNA barcoding. Int J Legal Med. 2014;1–8.
75.
Baker DA. DNA barcode identification of black cohosh herbal dietary supplements. J AOAC Int. 2012;95:1023–34.PubMedCrossRef
76.
Stoeckle MY, Gamble CC, Kirpekar R, Young G, Ahmed S, Little DP. Commercial teas highlight plant DNA barcode identification successes and obstacles. Sci Rep. 2011;1:1–7.CrossRef
77.
Taberlet P, Coissac E, Pompanon F, Brochmann C, Willerslev E. Towards next-generation biodiversity assessment using DNA metabarcoding. Mol Ecol. 2012;21:2045–50.PubMedCrossRef
78.
Jørgensen T, Kjaer KH, Haile J, Rasmussen M, Boessenkool S, Andersen K, et al. Islands in the ice: detecting past vegetation on Greenlandic nunataks using historical records and sedimentary ancient DNA Meta-barcoding. Mol Ecol. 2012;21:1980–8.PubMedCrossRef
79.
Valentini A, Miquel C, Nawaz MA, Bellemain E, Coissac E, Pompanon F, et al. New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: the trnL approach. Mol Ecol Resour. 2009;9:51–60.PubMedCrossRef
80.
Veldman S, Otieno JN, van Andel T, Gravendeel B, de Boer HJ. Efforts urged to tackle thriving illegal orchid trade in Tanzania and Zambia for chikanda production. Traffic Bull. 2014;26:47–50.
81.
Reja V, Kwok A, Stone G, Yang L, Missel A, Menzel C, et al. ScreenClust: advanced statistical software for supervised and unsupervised high resolution melting (HRM) analysis. Methods. 2010;50:S10–4.PubMedCrossRef
82.
Ririe KM, Rasmussen RP, Wittwer CT. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem. 1997;245:154–60.PubMedCrossRef
83.
Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem. 2003;49:853–60.PubMedCrossRef
84.
Osathanunkul M, Madesis P, de Boer HJ. Bar-HRM for authentication of plant-based medicines: evaluation of three medicinal products derived from Acanthaceae species. PLoS One. 2015;10(5):e0128476.PubMedCentralPubMedCrossRef
85.
Madesis P, Ganopoulos I, Sakaridis I, Argiriou A, Tsaftaris A. Advances of DNA-based methods for tracing the botanical origin of food products. Food Res Int. 2014;60:163–72.CrossRef
86.
Ganopoulos I, Madesis P, Darzentas N, Argiriou A, Tsaftaris A. Barcode High Resolution Melting (Bar-HRM) analysis for detection and quantification of PDO “Fava Santorinis” (Lathyrus clymenum) adulterants. Food Chem. 2012;133:505–12.PubMedCrossRef
87.
Ganopoulos I, Bazakos C, Madesis P, Kalaitzis P, Tsaftaris A. Barcode DNA high-resolution melting (Bar-HRM) analysis as a novel close-tubed and accurate tool for olive oil forensic use. J Sci Food Agric. 2013;93:2281–6.PubMedCrossRef
88.
Ganopoulos I, Xanthopoulou A, Mastrogianni A, Drouzas A, Kalivas A, Bletsos F, et al. High resolution melting (HRM) analysis in eggplant (Solanum melongena L.): a tool for microsatellite genotyping and molecular characterization of a Greek Genebank collection. Biochem Syst Ecol. 2015;58:64–71.CrossRef
89.
Kalivas A, Ganopoulos I, Xanthopoulou A, Chatzopoulou P, Tsaftaris A, Madesis P. DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece. Mol Biol Rep. 2014;41:5147–55.PubMedCrossRef
90.
Schmiderer C, Mader E, Novak J. DNA-based identification of Helleborus niger by high-resolution melting analysis. Planta Med. 2010;76:1934–7.PubMedCrossRef
91.
Ganopoulos I, Aravanopoulos F, Madesis P, Pasentsis K, Bosmali I, Ouzounis C, et al. Taxonomic identification of Mediterranean pines and their hybrids based on the high resolution melting (HRM) and trnL approaches: from cytoplasmic inheritance to timber tracing. PLoS One. 2013;8:e60945.PubMedCentralPubMedCrossRef
92.
Madesis P, Ganopoulos I, Bosmali I, Tsaftaris A. Barcode High Resolution Melting analysis for forensic uses in nuts: a case study on allergenic hazelnuts (Corylus avellana). Food Res Int. 2013;50:351–60.CrossRef
93.
Madesis P, Ganopoulos I, Anagnostis A, Tsaftaris A. The application of Bar-HRM (Barcode DNA-High Resolution Melting) analysis for authenticity testing and quantitative detection of bean crops (Leguminosae) without prior DNA purification. Food Control. 2012;25:576–82.CrossRef
94.
Bosmali I, Ganopoulos I, Madesis P, Tsaftaris A. Microsatellite and DNA-barcode regions typing combined with High Resolution Melting (HRM) analysis for food forensic uses: a case study on lentils (Lens culinaris). Food Res Int. 2012;46:141–7.CrossRef
95.
Ganopoulos I, Madesis P, Tsaftaris A. Universal ITS2 barcoding DNA region coupled with high-resolution melting (HRM) analysis for seed authentication and adulteration testing in leguminous forage and pasture species. Plant Mol Biol Rep. 2012;30:1322–8.CrossRef
96.
Lai G-H, Chao J, Lin M-K, Chang W-T, Peng W-H, Sun F-C, et al. Rapid and sensitive identification of the herbal tea ingredient Taraxacum formosanum using loop-mediated isothermal amplification. Int J Mol Sci. 2015;16:1562–75.PubMedCentralPubMedCrossRef
97.
Novak J, Grausgruber-Gröger S, Lukas B. DNA-based authentication of plant extracts. Food Res Int. 2007;40:388–92.CrossRef
98.
Smyth RP, Schlub TE, Grimm A, Venturi V, Chopra A, Mallal S, et al. Reducing chimera formation during PCR amplification to ensure accurate genotyping. Gene. 2010;469:45–51.PubMedCrossRef
99.
Lammers Y, Peelen T, Vos RA, Gravendeel B. The HTS barcode checker pipeline, a tool for automated detection of illegally traded species from high-throughput sequencing data. BMC Bioinform. 2014;15:44.CrossRef
100.
101.
102.
Palhares RM, Gonçalves Drummond M, dos Santos Alves Figueiredo Brasil B, Pereira Cosenza G, das Graças Lins Brandão M, Oliveira G. Medicinal plants recommended by the World Health Organization: DNA barcode identification associated with chemical analyses guarantees their quality. PLoS ONE 2015;10(5):e0127866. doi:10.​1371/​journal.​pone.​0127866.
Metadaten
Titel
DNA Barcoding and Pharmacovigilance of Herbal Medicines
verfasst von
Hugo J. de Boer
Mihael C. Ichim
Steven G. Newmaster
Publikationsdatum
01.07.2015
Verlag
Springer International Publishing
Erschienen in
Drug Safety / Ausgabe 7/2015
Print ISSN: 0114-5916
Elektronische ISSN: 1179-1942
DOI
https://doi.org/10.1007/s40264-015-0306-8

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