In summary, we reported the draft genome sequences of two clinical MDR-AB strains in Iran.
A. baumannii is known with its high resistance to carbapenems worldwide [
1,
9]. In a national Iranian study, the mean prevalence of MDR-AB was 71% during the period 2010–2015 [
2]. In the Taleghani Burn Hospital of our study, this prevalence increased with 92.5% of
A. baumannii carbapenem resistant [
10]. Carbapenem resistance in
A. baumannii was principally due to the production of oxacillinases including OXA-23, OXA-24, OXA-58 enzymes, and the New Delhi Metallo-β-lactamase 1 (NDM-1) [
1]. OXA-23 enzymes were predominant among MDR-AB in several regions worldwide [
9]. The international clone II corresponding to ST 2 of the Pasteur MLST scheme was associated with nosocomial outbreaks and had spread globally [
9,
11]. As with Europe, the spread of MDR-AB clonage lineage II producing
blaoxa23-like and
blaoxa24-like was described in Iran [
12]. Our results confirmed these studies with the description of one MDR-AB strain 554L carrying
blaoxa23 gene belonging to ST 2 and the second strain 554S harbouring
blaoxa72 (
blaoxa24-like) gene belonging to ST 307. Although isolated from the same blood culture sample, these two strains were phenotypically and genetically different. The different ST and the weak percentage of similarity between these strains confirmed that we had two different clones of
A. baumannii in the same sample. These isolates were resistant to several antibiotics and only tigecycline and colistin were effective on both strains. Each strain carried different resistance genes, with the
blaOXA-72 encoding carbapenem resistance gene found in the isolate 554S and the
blaOXA-23 gene present in the strain 554L. The co-occurrence of different carbapenemase genes in the same
Acinetobacter strain has already been described [
13]. However, few genome sequence analyses explain this phenomenon. We could observe one carbapenemase encoding gene in a chromosome and one in a plasmid [
14], or both in a chromosome [
13]. In our case, without a careful observation of the colonies’ morphological aspect, we might conclude the presence of one strain of
A. baumannii harboring two carbapenemases genes (
blaOXA-23 and
blaOXA-72), whereas we had two different
A. baumannii strains, each harboring one carbapenemase gene. Hence, this study has revealed the possibility that double carbapenemase producers previously reported in the literature could in fact be a dual infection. Further work is warranted to understand if this phenomenon is common in human infections. These data also revealed molecular mechanisms contributing to bacterial drug resistance dissemination and expanded our understanding of the genomic features of MDR-AB in Iran. To our knowledge, this was the first draft genome sequences of MDR-AB isolates in Iran.
These Whole Genome Shotgun projects have been deposited in DDBJ/EMBL/GenBank under the sequence accession numbers NKXP00000000 and NKXQ00000000 for strains 554S and 554L, respectively.