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Diabetologia
Erschienen in: Diabetologia 6/2004

01.06.2004 | Article

Early vitamin E supplementation attenuates diabetes-associated vascular dysfunction and the rise in protein kinase C-β in mesenteric artery and ameliorates wall stiffness in femoral artery of Wistar rats

verfasst von: S. J. Wigg, M. Tare, J. Forbes, M. E. Cooper, M. C. Thomas, H. A. Coleman, H. C. Parkington, R. C. O’Brien

Erschienen in: Diabetologia | Ausgabe 6/2004

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Abstract

Aims/hypothesis

The impact of early vitamin E supplementation on vascular function in diabetes remains unresolved. Therefore, we examined the effects of vitamin E on functional and structural parameters and on chemical markers that are disturbed in diabetes in mesenteric and femoral arteries.

Methods

Segments of both arteries, taken from control and 8-week-old streptozotocin diabetic Wistar rats that were treated or not with vitamin E, were mounted on wire and pressure myographs, after which endothelium-dependent and -independent vasodilation was assessed. Passive mechanical wall properties and the localisation and levels of protein kinase C (PKC)-β2 and AGE were evaluated in these vessels.

Results

Vitamin E supplementation was associated with improved endothelium-dependent and -independent vasodilatation in mesenteric arteries from diabetic rats. Impaired endothelium-dependent vasodilatation in diabetic mesenteric vessels was associated with PKC-β2 up-regulation and this was prevented by vitamin E supplementation. Increased AGE accumulation and plasma isoprostane levels in diabetic rats were not changed by vitamin E. In the femoral artery, vitamin E supplementation had no effect on endothelium-dependent or -independent vasodilatation, but did prevent the wall stiffening associated with diabetes.

Conclusions/interpretation

Early vitamin E supplementation has a beneficial effect on diabetes-induced endothelial dysfunction in resistance arteries. This benefit may arise from a direct effect on smooth muscle function, as a result of inhibition of the PKC-β2 isoform by vitamin E.
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Metadaten
Titel
Early vitamin E supplementation attenuates diabetes-associated vascular dysfunction and the rise in protein kinase C-β in mesenteric artery and ameliorates wall stiffness in femoral artery of Wistar rats
verfasst von
S. J. Wigg
M. Tare
J. Forbes
M. E. Cooper
M. C. Thomas
H. A. Coleman
H. C. Parkington
R. C. O’Brien
Publikationsdatum
01.06.2004
Verlag
Springer-Verlag
Erschienen in
Diabetologia / Ausgabe 6/2004
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI
https://doi.org/10.1007/s00125-004-1411-x

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