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Lasers in Medical Science

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01.01.2011 | Original Article

Effect and mechanism of 5-aminolevulinic acid-mediated photodynamic therapy in esophageal cancer

verfasst von: Xiaohua Chen, Peng Zhao, Fengsheng Chen, Libo Li, Rongcheng Luo

Erschienen in: Lasers in Medical Science | Ausgabe 1/2011

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Abstract

5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) provides a novel and promising treatment for esophageal cancer. However, its specific mechanism has not been fully elucidated and its efficacy is remarkably varied. This study investigated the effect of ALA-PDT on esophageal squamous carcinoma cell line Eca-109 in vitro and vivo to explore optimal parameters, and evaluated the significance of cell apoptosis, cell cycle, ALA-protoporphyrin IX (ALA-PpIX) subcellular localization, and expression of Bcl-2 and Bax mRNA in cells to understand the mechanism of ALA-PDT for esophageal cancer. How ALA concentration, incubation time, and laser irradiation dose influenced the cell proliferation was determined by MTT assay. ALA-PpIX subcellular localization was analyzed by confocal microscopy. The mRNA changes were detected by quantitative real-time polymerase chain reaction (QRT-PCR). Tumor models transplanted with Eca-109 cells in nude mice were established (n = 10) and killed (n = 4) at 24 h post-PDT for malondialdehyde (MDA) detection and histological study. The remaining mice were measured the tumor size for 3 weeks after treatment. Our data show that ALA-PDT significantly inhibits cell proliferation (p < 0.05), the PDT efficacy depends on the saturation of ALA concentration, incubation time, and laser irradiation dose, and the best effect in tumor destruction is at 7–14 days post-PDT. ALA-PpIX is localized in mitochondria and cytoplasm. ALA-PDT induces cell apoptosis and arrests cell cycle at G0/G1 phase. Bcl-2 is significantly down-regulated while Bax is up-regulated (p < 0.05). The results of this study provide references in choosing clinical optimal PDT parameters and help in better understanding the PDT mechanism for esophageal cancer.

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Titel: Effect and mechanism of 5-aminolevulinic acid-mediated photodynamic therapy in esophageal cancer
verfasst von: Xiaohua Chen
Peng Zhao
Fengsheng Chen
Libo Li
Rongcheng Luo
Publikationsdatum: 01.01.2011
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 1/2011
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-010-0810-0

Springer Medizin

Abstract

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