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Lasers in Medical Science

Erschienen in:

01.01.2015 | Original Article

Effect of DTPP-mediated photodynamic therapy on cell morphology, viability, cell cycle, and cytotoxicity in a murine lung adenocarcinoma cell line

verfasst von: Jianhua Liu, Liqing Zheng, Yingxin Li, Zhihua Zhang, Li Zhang, Lixia Shen, Xiulong Zhang, Haixia Qiao

Erschienen in: Lasers in Medical Science | Ausgabe 1/2015

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Abstract

Photodynamic therapy (PDT) involves the administration and activation of photosensitizing reagents in cancer tissues to induce cytotoxicity. Here we examined the effects of 5-5- (4-N, N-diacetoxylphenyl)-10,15,20- tetraphenylporphyrin (DTPP) -mediated PDT on cell morphology, viability, cell cycle, and cytotoxicity in a murine lung adenocarcinoma cell line. LA795 murine lung adenocarcinoma cell line was used in the study, with cellular uptake of DTPP being quantified by a UV-visible spectrophotometer. The subcellular localization of DTPP was detected by confocal laser scanning microscopy, alteration of cell morphology after PDT was observed by an inverted light microscope, and late-stage apoptosis was examined by terminal dUTP nick end labeling (TUNEL) . The effects of influencing factors on cytotoxicity of PDT in LA795 cells was investigated with varying concentrations of DTPP, energy densities, power densities, and antioxidants by 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Effects of PDT on cell cycle and plasma membrane integrity were studied by flow cytometry analysis. The uptake of DTPP by LA795 cells reached maximum after incubation for 24 h. Confocal laser scanning microscopy showed that DTPP was mainly in the mitochondrion, and slight localization was detected in the lysosomes. Cellular inhibitory effects increased with increased irradiation dose and DTPP concentration, while unactivated DTPP had low toxicity. Flow cytometry analysis revealed that DTPP-PDT-treated cells showed S phase arrest. Cell membrane damage initiation, repair, and irreversible damage were observed at 2, 4, and 5 h after DTPP-PDT , respectively. Together, our results demonstrated cell apoptosis, compromised viability, and cell cycle S phase arrest of LA795 in response to DTPP-PDT , while no effect on the lung cancer cells was observed with irradiation or photosensitizer treatment alone.

Marks P (1999) Photodynamic therapy for central nervous system tumors: achievements and prospects. Br J Neurosurg 13(4):349–351PubMedCrossRef

Marks PV, Belchetz PE, Saxena A et al (2000) Effect of photo-dynamic therapy on recurrent pituitary adenomas: clinical phase I/ II trial—an early report. Br J Neurosurg 14:317–325

Allison RR, Sibata CH (2010) Oncological photodynamic therapy photosensitizers: a clinical review. Photodiagn Photodyn Ther 7:61–75CrossRef

Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D (2011) Global cancer statistics. CA Cancer J Clin 61:69–90PubMedCrossRef

Kessel D, Luo Y, Mathieu P, Reiners JJ Jr (2000) Determinants of apoptotic response to lysosomal photodamage. Photochem Photobiol 71:196–200PubMedCrossRef

Kessel D, Vicente MG, Reiners JJ (2006) Initiation of apoptosis and autophagy by photodynamic therapy. Lasers Surg Med 38:482–488PubMedCentralPubMedCrossRef

Chi L (2005) Technological developments in laser-guided cancer screening and therapy. Univ Toronto Med J 83:119–120

Dougherty TJ, Gomer CJ, Henderson BW, Jori G, Kessel D, Korbelik M, Moan J, Peng Q (1998) Photodynamic therapy. J Natl Cancer Inst 90:889–905PubMedCrossRef

Yang VXD, Muller PJ, Herman P, Wilson BC (2003) A multispectral fluorescence imaging system: design and initial clinic tests in intra-operative Photofrin-photodynamic therapy of brain tumors. Lasers Surg Med 32(3):224–232PubMedCrossRef

10.

Chen J, Wang Y, Liu T (2011) Photodynamic efficiency of new porphyrin-typed drug on HGC27 and MGC803 cells. Int J Biomed Eng 34(3):154–157

11.

Yslas E, Rivarola V, Durantini EN (2005) Synthesis and photodynamic activity of zinc(II) phthalocyanine derivatives bearing methoxy and trifluoromethylbenzyloxy substituents in homogeneous and biological media. Bioorg Med Chem 13:39–46PubMedCrossRef

12.

Macdonald IJ, Dougherty TJ (2001) Basic principles of photodynamic therapy. J Porphyr Phthalocyan 5:105–129CrossRef

13.

Krishnamurthy S, Powers SK, Witmer P, Brown T (2000) Optimal light dose for interstitial photodynamic therapy in treatment for malignant brain tumors. Lasers Surg Med 27:224–234PubMedCrossRef

14.

Tomioka Y, Kushibiki T, Awazu K (2010) Evaluation of oxygen consumption of culture medium and in vitro photodynamic effect of talaporfin sodium in lung tumor cells. Photomed Laser Surg 28:385–390

15.

Buytaert E, Dewaele M, Agostinis P (2007) Molecular effectors of multiple cell death pathways initiated by photodynamic therapy. Biochim Biophy Acta 1776:86–107

16.

Henderson BW, Busch TM, Snyder JW (2006) Fluence rate as a modulator of PDT mechanisms. Lasers Surg Med 38:489–493PubMedCrossRef

17.

Harrod-Kim P (2006) Tumor ablation with photodynamic therapy: introduction to mechanism and clinical applications. J Vasc Interv Radiol 17:1441–1448PubMedCrossRef

18.

Zhu TC, Finlay JC (2008) The role of photodynamic therapy (PDT) physics. Med Phys 35:3127–3136PubMedCentralPubMedCrossRef

19.

Yin H, Li Y, Zheng Y, Ye X, Zheng L, Li C, Xue Z (2012) Photoinactivation of cell-free human immuno deficiency virus by hematoporphyrin monomethyl ether. Lasers Med Sci 27:943–950PubMedCentralPubMedCrossRef

20.

Gruber J, Fong S, Chen C-B , Yoong S, Pastorin G, Schaffer S, Cheah I, Halliwell B (2013) Mitochondria-targeted antioxidants and metabolic modulators as pharmacological interventions to slow ageing. Biotechnol Adv 31:563–592PubMedCrossRef

21.

Pazos MC, Nader HB (2007) Effect of photodynamic therapy on the extracellular matrix and associated components. Braz J Med Biol Res 40:1025–1035PubMedCrossRef

22.

Ratcliffe SL, Matthews EK (1995) Modification of the photodynamic action of delta-aminolaevulinic acid (ALA) on rat pancreatoma cells by mitochondrial benzodiazepine receptor ligands. Br J Cancer 71:300–305PubMedCentralPubMedCrossRef

23.

Verma A, Facchina SL, Hirsch DJ, Song SY, Dillahey LF, Williams JR, Snyder SH (1998) Photodynamic tumor therapy: mitochondrial benzodiazepine receptors as a therapeutic target. Mol Med 4:40–45PubMedCentralPubMed

24.

Kessel D, Luo Y (1998) Mitochondrial photodamage and PDT-induced apoptosis. J Photochem Photobiol B 42:89–95PubMedCrossRef

25.

Noodt BB, Berg K, Stokke T, Peng Q, Nesland JM (1999) Different apoptotic pathways are induced from various intracellular sites by tetraphenylporphyrins and light. Br J Cancer 79:72–81PubMedCentralPubMedCrossRef

26.

Kessel D, Luo Y, Deng Y, Chang CK (1997) The role of subcellular localization in initiation of apoptosis by photodynamic therapy. Photochem Photobiol 65:422–426PubMedCrossRef

27.

Haywood-Smalla SL, Vernona DI, Griffithsb J, Schofieldb J, Brown SB (2006) Phthalocyanine-mediated photodynamic therapy induces cell death and a G0/G1 cell cycle arrest in cervical cancer cells. Biochem Biophys Res Commun 339:569–576CrossRef

28.

Meikrantz W, Gisselbrecht S, Tam SW, Schlegel R (1994) Activation of cyclin A-dependent protein kinases during apoptosis. Natl Acad Sci 91(9):3754–3758CrossRef

29.

Lukšienė Z (2003) Photodynamic therapy: mechanism of action and ways to improve the efficiency of treatment. Medicina 39:1137–1150PubMed

30.

Van Hillegers R, Kort WJ, Wilson JHP (1994) Current status of photodynamic therapy in oncology. Drugs 48:510–527CrossRef

31.

Yao JA, Jiang M, Tseng GN (1997) Mechanism of enhancement of slow delayed rectifier current by extracellular sulfhydryl modification [J]. Am J Physiol 273:208–219

32.

Chiamvimonvat N, O’Rourke B, Kamp TJ et al (1995) Functional consequences of sulfhydryl modification in the pore-forming subunits of cardiovascular Ca2+ and Na+ channels [J]. Circ Res 76:325–334

33.

Xianting L, Cheng Y (2013) Enantiomer-specific profenofos-induced cytotoxicity and DNA damage mediated by oxidative stress in rat adrenal pheochromocytoma (PC12) cells. J Appl Toxicol 34(2):166–175

Titel: Effect of DTPP-mediated photodynamic therapy on cell morphology, viability, cell cycle, and cytotoxicity in a murine lung adenocarcinoma cell line
verfasst von: Jianhua Liu
Liqing Zheng
Yingxin Li
Zhihua Zhang
Li Zhang
Lixia Shen
Xiulong Zhang
Haixia Qiao
Publikationsdatum: 01.01.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1637-x

Springer Medizin

Abstract

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