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Clinical Oral Investigations
Erschienen in: Clinical Oral Investigations 2/2015

01.03.2015 | Original Article

Effect of growth factors on antimicrobial peptides and pro-inflammatory mediators during wound healing

verfasst von: H. Dommisch, J. Winter, W. Götz, J. Miesen, A. Klein, L. Hierse, J. Deschner, A. Jäger, J. Eberhard, S. Jepsen

Erschienen in: Clinical Oral Investigations | Ausgabe 2/2015

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Abstract

Background

Antimicrobial peptides (AMPs), such as human beta-defensin-2 (hBD-2) and the CC-chemokine ligand 20 (CCL20), exhibit direct microbicidal effects and mediator-like activity. It was hypothesized that wounding induces the expression of AMPs and pro-inflammatory mediators and that endogenous mediators, such as insulin-like growth factor-1 (IGF-1) and transforming growth factor-alpha (TGF-alpha), modulate this induced expression.

Material and methods

Monolayers of gingival epithelial cells (GECs) and gingival fibroblast (HGFs) from three different donors were wounded using the scratch assay (in vitro wounding) in the presence (test group) or absence (control group) of IGF-1 and TGF-alpha. In vitro wound closure was monitored over time (0, 6, 24, 48, 72 h), and wound areas were microscopically analyzed (Axio-Vision® Software, Zeiss). Gene expression analysis of the GAPDH, hBD-2, CCL20, interleukin-1 beta (IL-1 beta), and interleukin-8 (IL-8) was performed by qPCR.

Results

In comparison to control cells, IGF-1 and TGF-alpha significantly enhanced in vitro wound closure (P < 0.05). In GECs, IGF-1 induced the gene expression of IL-1 beta and IL-8 when compared to control cells (P < 0.05). In HGFs, wounding per se induced the messenger RNA of hBD-2, CCL20, and IL-1 beta, whereas IGF-1 and TGF-alpha reversed this effect (P < 0.05).

Conclusion

In gingival cells, the gene expression of AMPs was altered by injury, and endogenous growth factors further influenced the expression profiles, but with high interindividual differences.
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Literatur
1.
2.
3.
4.
5.
Han X, Amar S (2003) IGF-1 signaling enhances cell survival in periodontal ligament fibroblasts vs. gingival fibroblasts. J Dental Res 82:454–9CrossRef
6.
Bennett NT, Schultz GS (1993) Growth factors and wound healing: biochemical properties of growth factors and their receptors. Ame J Surg 165:728–737CrossRef
7.
Marikovsky M, Vogt P, Eriksson E, Rubin JS, Taylor WG, Joachim S, Klagsbrun M (1996) Wound fluid-derived heparin-binding EGF-like growth factor (HB-EGF) is synergistic with insulin-like growth factor-I for Balb/MK keratinocyte proliferation. J Invest Dermatol 106:616–621CrossRefPubMed
8.
Pierre EJ, Perez-Polo JR, Mitchell AT, Matin S, Foyt HL, Herndon DN (1997) Insulin-like growth factor-I liposomal gene transfer and systemic growth hormone stimulate wound healing. J Burn Care & Rehabil 18:287–291CrossRef
9.
Steenfos HH (1994) Growth factors and wound healing. Scand J Plast Reconstr Surg Hand Surg / Nordisk Plastikkirurgisk Forening [and] Nordisk klubb for Handkirurgi 28:95–105CrossRef
10.
Brown DL, Kane CD, Chernausek SD, Greenhalgh DG (1997) Differential expression and localization of insulin-like growth factors I and II in cutaneous wounds of diabetic and nondiabetic mice. Am J Pathol 151:715–724PubMedCentralPubMed
11.
12.
Hodak E, Gottlieb AB, Anzilotti M, Krueger JG (1996) The insulin-like growth factor 1 receptor is expressed by epithelial cells with proliferative potential in human epidermis and skin appendages: correlation of increased expression with epidermal hyperplasia. J Invest Dermatol 106:564–570CrossRefPubMed
13.
Andresen JL, Ehlers N (1998) Chemotaxis of human keratocytes is increased by platelet-derived growth factor-BB, epidermal growth factor, transforming growth factor-alpha, acidic fibroblast growth factor, insulin-like growth factor-I, and transforming growth factor-beta. Curr Eye Res 17:79–87CrossRefPubMed
14.
15.
Kim I, Mogford JE, Chao JD, Mustoe TA (2001) Wound epithelialization deficits in the transforming growth factor-alpha knockout mouse. Wound Repair Regen 9:386–390CrossRefPubMed
16.
Kheradmand F, Folkesson HG, Shum L, Derynk R, Pytela R, Matthay MA (1994) Transforming growth factor-alpha enhances alveolar epithelial cell repair in a new in vitro model. Am J Physiol 267:L728–738PubMed
17.
Dale BA, Kimball JR, Krisanaprakornkit S, Roberts F, Robinovitch M, O’Neal R, Valore EV, Ganz T, Anderson GM, Weinberg A (2001) Localized antimicrobial peptide expression in human gingiva. J Periodontal Res 36:285–294CrossRefPubMed
18.
Dale BA (2002) Periodontal epithelium: a newly recognized role in health and disease. Periodontol 2000(30):70–78CrossRef
19.
Dale BA (2003) Fascination with epithelia: architecture, proteins, and functions. J Dent Res 82:866–869CrossRefPubMed
20.
Dale BA, Fredericks LP (2005) Antimicrobial peptides in the oral environment: expression and function in health and disease. Curr Issues Mol Biol 7:119–133PubMedCentralPubMed
21.
22.
Chung WO, Dommisch H, Yin L, Dale BA (2007) Expression of defensins in gingiva and their role in periodontal health and disease. Curr Pharm Des 13:3073–3083CrossRefPubMed
23.
Yang D, Biragyn A, Hoover DM, Lubkowski J, Oppenheim JJ (2004) Multiple roles of antimicrobial defensins, cathelicidins, and eosinophil-derived neurotoxin in host defense. Annu Rev Immunol 22:181–215CrossRefPubMed
24.
Dunsche A, Acil Y, Dommisch H, Siebert R, Schroder JM, Jepsen S (2002) The novel human beta-defensin-3 is widely expressed in oral tissues. Eur J Oral Sci 110:121–124CrossRefPubMed
25.
Dommisch H, Acil Y, Dunsche A, Winter J, Jepsen S (2005) Differential gene expression of human beta-defensins (hBD-1, -2, -3) in inflammatory gingival diseases. Oral Microbiol Immunol 20:186–190CrossRefPubMed
26.
Rizzo A, Paolillo R, Buommino E, Lanza AG, Guida L, Annunziata M, Carratelli CR (2008) Modulation of cytokine and beta-defensin 2 expressions in human gingival fibroblasts infected with Chlamydia pneumoniae. Int Immunopharmacol 8:1239–1247. doi:10.​1016/​j.​intimp.​2008.​04.​015 CrossRefPubMed
27.
28.
Hoover DM, Boulegue C, Yang D, Oppenheim JJ, Tucker K, Lu W, Lubkowski J (2002) The structure of human macrophage inflammatory protein-3alpha/CCL20. Linking antimicrobial and CC chemokine receptor-6-binding activities with human beta-defensins. J Biol Chem 277:37647–37654CrossRefPubMed
29.
Yang D, Chertov O, Bykovskaia SN, Chen Q, Buffo MJ, Shogan J, Anderson M, Schroder JM, Wang JM, Howard OM, Oppenheim JJ (1999) Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science 286:525–528CrossRefPubMed
30.
Schutyser E, Struyf S, Van Damme J (2003) The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev 14:409–426CrossRefPubMed
31.
Yang D, Chen Q, Chertov O, Oppenheim JJ (2000) Human neutrophil defensins selectively chemoattract naive T and immature dendritic cells. J Leukoc Biol 68:9–14PubMed
32.
Hasturk H, Kantarci A, Goguet-Surmenian E, Blackwood A, Andry C, Serhan CN, Van Dyke TE (2007) Resolvin E1 regulates inflammation at the cellular and tissue level and restores tissue homeostasis in vivo. J Immunol 179:7021–7029CrossRefPubMed
33.
34.
Schroder JM, Christophers E (1992) The biology of NAP-1/IL-8, a neutrophil-activating cytokine. Immunol Ser 57:387–416PubMed
35.
Dommisch H, Chung WO, Rohani MG, Williams D, Rangarajan M, Curtis MA, Dale BA (2007) Protease-activated receptor 2 mediates human beta-defensin 2 and CC chemokine ligand 20 mRNA expression in response to proteases secreted by Porphyromonas gingivalis. Infect Immun 75:4326–4333CrossRefPubMedCentralPubMed
36.
Tokumaru S, Sayama K, Shirakata Y, Komatsuzawa H, Ouhara K, Hanakawa Y, Yahata Y, Dai X, Tohyama M, Nagai H, Yang L, Higashiyama S, Yoshimura A, Sugai M, Hashimoto K (2005) Induction of keratinocyte migration via transactivation of the epidermal growth factor receptor by the antimicrobial peptide LL-37. J Immunol 175:4662–4668CrossRefPubMed
37.
38.
39.
40.
41.
42.
Elijah IE, Branski LK, Finnerty CC, Herndon DN (2011) The GH/IGF-1 system in critical illness. Best Practice &amp; Research Clinical Endocrinology &amp. Metabolism 25:759–767. doi:10.​1016/​j.​beem.​2011.​06.​002
43.
Oberringer M, Meins C, Bubel M, Pohlemann T (2008) In vitro wounding: effects of hypoxia and transforming growth factor beta1 on proliferation, migration and myofibroblastic differentiation in an endothelial cell-fibroblast co-culture model. J Mol Histol 39:37–47. doi:10.​1007/​s10735-007-9124-3 CrossRefPubMed
44.
Wang L, Ko CY, Meyers EE, Pedroja BS, Pelaez N, Bernstein AM (2011) Concentration-dependent effects of transforming growth factor beta1 on corneal wound healing. Mol Vision 17:2835–2846
45.
Tall EG, Bernstein AM, Oliver N, Gray JL, Masur SK (2010) TGF-beta-stimulated CTGF production enhanced by collagen and associated with biogenesis of a novel 31-kDa CTGF form in human corneal fibroblasts. Invest Ophthalmol Vis Sci 51:5002–5011. doi:10.​1167/​iovs.​09-5110 CrossRefPubMedCentralPubMed
46.
Garlick JA, Parks WC, Welgus HG, Taichman LB (1996) Re-epithelialization of human oral keratinocytes in vitro. J Dental Res 75:912–918CrossRef
47.
Niyonsaba F, Ushio H, Nakano N, Ng W, Sayama K, Hashimoto K, Nagaoka I, Okumura K, Ogawa H (2007) Antimicrobial peptides human beta-defensins stimulate epidermal keratinocyte migration, proliferation and production of proinflammatory cytokines and chemokines. J Invest Dermatol 127:594–604CrossRefPubMed
48.
Niyonsaba F, Ogawa H, Nagaoka I (2004) Human beta-defensin-2 functions as a chemotactic agent for tumour necrosis factor-alpha-treated human neutrophils. Immunology 111:273–281CrossRefPubMedCentralPubMed
49.
Metadaten
Titel
Effect of growth factors on antimicrobial peptides and pro-inflammatory mediators during wound healing
verfasst von
H. Dommisch
J. Winter
W. Götz
J. Miesen
A. Klein
L. Hierse
J. Deschner
A. Jäger
J. Eberhard
S. Jepsen
Publikationsdatum
01.03.2015
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Oral Investigations / Ausgabe 2/2015
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-014-1239-9

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